(Linear) (Six-base) MAPSORT of: KIEE0217.seq  Check: 7139  from: 1  to: 516



KIEE0217.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:      0     179     516 
   Size:        179     337
 
AclI AA'CG_TT

Cuts at:      0     457     516 
   Size:        457      59
 
AflIII A'CryG_T

Cuts at:      0      89     516 
   Size:         89     427
 
ApoI r'AATT_y

Cuts at:      0     294     380     516 
   Size:        294      86     136

  Fragments arranged by size: 

                 294     136      86
 
BanII G_rGCy'C

Cuts at:      0      73     516 
   Size:         73     443
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     236     516 
   Size:        236     280
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       7      41     516 
   Size:          7      34     475

  Fragments arranged by size: 

                 475      34       7
 
BglI GCCn_nnn'nGGC

Cuts at:      0     247     516 
   Size:        247     269
 
Bme1580I G_kGCm'C

Cuts at:      0     128     516 
   Size:        128     388
 
BmgBI CAC'GTC

Cuts at:      0      90     516 
   Size:         90     426
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     481     516 
   Size:         59     422      35

  Fragments arranged by size: 

                 422      59      35
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     138     485     516 
   Size:        138     347      31

  Fragments arranged by size: 

                 347     138      31
 
BsaBI GATnn'nnATC

Cuts at:      0     172     516 
   Size:        172     344
 
BsiHKAI G_wGCw'C

Cuts at:      0      73     395     516 
   Size:         73     322     121

  Fragments arranged by size: 

                 322     121      73
 
BsmI GAATG_Cn'

Cuts at:      0     449     516 
   Size:        449      67
 
Bsp1286I G_dGCh'C

Cuts at:      0      73     128     395     516 
   Size:         73      55     267     121

  Fragments arranged by size: 

                 267     121      73      55
 
BtgI C'CryG_G

Cuts at:      0      29     516 
   Size:         29     487
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      38     430     516 
   Size:         38     392      86

  Fragments arranged by size: 

                 392      86      38
 
DraI TTT'AAA

Cuts at:      0      56     516 
   Size:         56     460
 
EaeI y'GGCC_r

Cuts at:      0      14      48     516 
   Size:         14      34     468

  Fragments arranged by size: 

                 468      34      14
 
EcoICRI GAG'CTC

Cuts at:      0      71     516 
   Size:         71     445
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     481     516 
   Size:         59     422      35

  Fragments arranged by size: 

                 422      59      35
 
HindIII A'AGCT_T

Cuts at:      0     508     516 
   Size:        508       8
 
MfeI C'AATT_G

Cuts at:      0     467     516 
   Size:        467      49
 
MscI TGG'CCA

Cuts at:      0      50     516 
   Size:         50     466
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     121     516 
   Size:        121     395
 
SacI G_AGCT'C

Cuts at:      0      73     516 
   Size:         73     443
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     410     516 
   Size:        410     106
 
TatI w'GTAC_w

Cuts at:      0      92     516 
   Size:         92     424
 

 Enzymes that do cut:

     AccI     AclI   AflIII     ApoI    BanII     BbsI     BcgI     BglI
 Bme1580I    BmgBI     BpmI     BsaI    BsaBI  BsiHKAI     BsmI Bsp1286I
     BtgI    BtgZI     DraI     EaeI  EcoICRI  Eco57MI  HindIII     MfeI
     MscI    PflMI     SacI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI