(Linear) (Six-base) MAPSORT of: KIEE0219.seq  Check: 2383  from: 1  to: 486



KIEE0219.orf

 With 182 enzymes: SgfI * 

                        October 6, 2008 10:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     486 
   Size:        365     121
 
ApoI r'AATT_y

Cuts at:      0     302     486 
   Size:        302     184
 
AvaI C'yCGr_G

Cuts at:      0      73      88     486 
   Size:         73      15     398

  Fragments arranged by size: 

                 398      73      15
 
BamHI G'GATC_C

Cuts at:      0     120     486 
   Size:        120     366
 
BbvCI CC'TCA_GC

Cuts at:      0     319     486 
   Size:        319     167
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     486 
   Size:         28     458
 
Bpu10I CC'TnA_GC

Cuts at:      0     319     486 
   Size:        319     167
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     486 
   Size:        130     356
 
BsaAI yAC'GTr

Cuts at:      0     246     486 
   Size:        246     240
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      34      64     486 
   Size:         34      30     422

  Fragments arranged by size: 

                 422      34      30
 
BseYI C'CCAG_C

Cuts at:      0     150     486 
   Size:        150     336
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     406     486 
   Size:        313      93      80

  Fragments arranged by size: 

                 313      93      80
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       4     486 
   Size:          4     482
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     370     486 
   Size:        370     116
 
BsrDI GCAATG_nn'

Cuts at:      0     293     419     486 
   Size:        293     126      67

  Fragments arranged by size: 

                 293     126      67
 
BsrFI r'CCGG_y

Cuts at:      0     128     177     486 
   Size:        128      49     309

  Fragments arranged by size: 

                 309     128      49
 
BsrGI T'GTAC_A

Cuts at:      0     251     486 
   Size:        251     235
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     285     486 
   Size:        285     201
 
BstYI r'GATC_y

Cuts at:      0     120     209     486 
   Size:        120      89     277

  Fragments arranged by size: 

                 277     120      89
 
EaeI y'GGCC_r

Cuts at:      0     275     486 
   Size:        275     211
 
EarI CTCTTCn'nnn_

Cuts at:      0      40     394     462     486 
   Size:         40     354      68      24

  Fragments arranged by size: 

                 354      68      40      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      37     486 
   Size:         37     449
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     365     486 
   Size:         28     337     121

  Fragments arranged by size: 

                 337     121      28
 
EcoRI G'AATT_C

Cuts at:      0     302     486 
   Size:        302     184
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     171     486 
   Size:        171     315
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     486 
   Size:         34      32     420

  Fragments arranged by size: 

                 420      34      32
 
MscI TGG'CCA

Cuts at:      0     277     486 
   Size:        277     209
 
MspA1I CmG'CkG

Cuts at:      0      68     323     486 
   Size:         68     255     163

  Fragments arranged by size: 

                 255     163      68
 
PspXI vC'TCGA_Gb

Cuts at:      0      73     486 
   Size:         73     413
 
PstI C_TGCA'G

Cuts at:      0     217     486 
   Size:        217     269
 
PvuII CAG'CTG

Cuts at:      0     323     486 
   Size:        323     163
 
SapI GCTCTTCn'nnn_

Cuts at:      0      40     486 
   Size:         40     446
 
SfcI C'TryA_G

Cuts at:      0      54     213     486 
   Size:         54     159     273

  Fragments arranged by size: 

                 273     159      54
 
SmlI C'TyrA_G

Cuts at:      0      73     145     486 
   Size:         73      72     341

  Fragments arranged by size: 

                 341      73      72
 
SnaBI TAC'GTA

Cuts at:      0     246     486 
   Size:        246     240
 
TatI w'GTAC_w

Cuts at:      0     251     486 
   Size:        251     235
 
XhoI C'TCGA_G

Cuts at:      0      73     486 
   Size:         73     413
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BamHI    BbvCI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaXI    BseYI     BsgI    BsmBI    BspMI    BsrDI    BsrFI
    BsrGI    BstXI    BstYI     EaeI     EarI     EciI  Eco57MI    EcoRI
     FalI    Hin4I     MscI   MspA1I    PspXI     PstI    PvuII     SapI
     SfcI     SmlI    SnaBI     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaHI
    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI
    BspHI    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI