(Linear) (Six-base) MAPSORT of: KIEE0220.seq Check: 1575 from: 1 to: 204 KIEE0220.orf With 182 enzymes: SgfI * April 24, 2009 13:11 .. ApoI r'AATT_y Cuts at: 0 94 204 Size: 94 110 AseI AT'TA_AT Cuts at: 0 182 204 Size: 182 22 BglI GCCn_nnn'nGGC Cuts at: 0 33 204 Size: 33 171 BstXI CCAn_nnnn'nTGG Cuts at: 0 60 204 Size: 60 144 BtgI C'CryG_G Cuts at: 0 110 204 Size: 110 94 EcoRI G'AATT_C Cuts at: 0 94 204 Size: 94 110 NcoI C'CATG_G Cuts at: 0 110 204 Size: 110 94 StyI C'CwwG_G Cuts at: 0 110 204 Size: 110 94 Enzymes that do cut: ApoI AseI BglI BstXI BtgI EcoRI NcoI StyI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI