(Linear) (Six-base) MAPSORT of: KIEE0225.seq  Check: 9758  from: 1  to: 354



KIEE0225.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     260     302     354 
   Size:        260      42      52

  Fragments arranged by size: 

                 260      52      42
 
BanII G_rGCy'C

Cuts at:      0     295     322     354 
   Size:        295      27      32

  Fragments arranged by size: 

                 295      32      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     108     354 
   Size:        108     246
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     279     354 
   Size:        122     157      75

  Fragments arranged by size: 

                 157     122      75
 
Bpu10I CC'TnA_GC

Cuts at:      0     205     354 
   Size:        205     149
 
BsaHI Gr'CG_yC

Cuts at:      0     237     354 
   Size:        237     117
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      26     354 
   Size:         26     328
 
Bsp1286I G_dGCh'C

Cuts at:      0     295     322     354 
   Size:        295      27      32

  Fragments arranged by size: 

                 295      32      27
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     286     313     340     354 
   Size:        286      27      27      14

  Fragments arranged by size: 

                 286      27      27      14
 
BtsI GCAGTG_nn'

Cuts at:      0      31     273     279     354 
   Size:         31     242       6      75

  Fragments arranged by size: 

                 242      75      31       6
 
EaeI y'GGCC_r

Cuts at:      0     276     354 
   Size:        276      78
 
EarI CTCTTCn'nnn_

Cuts at:      0     150     354 
   Size:        150     204
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     279     354 
   Size:        122     157      75

  Fragments arranged by size: 

                 157     122      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0     218     354 
   Size:        218     136
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     354 
   Size:        258      96
 
MscI TGG'CCA

Cuts at:      0     278     354 
   Size:        278      76
 
MspA1I CmG'CkG

Cuts at:      0     257     299     354 
   Size:        257      42      55

  Fragments arranged by size: 

                 257      55      42
 
PstI C_TGCA'G

Cuts at:      0     181     354 
   Size:        181     173
 
PvuII CAG'CTG

Cuts at:      0     257     299     354 
   Size:        257      42      55

  Fragments arranged by size: 

                 257      55      42
 
SfcI C'TryA_G

Cuts at:      0     177     354 
   Size:        177     177
 
TatI w'GTAC_w

Cuts at:      0      70     354 
   Size:         70     284
 

 Enzymes that do cut:

    AlwNI    BanII     BmrI     BpmI   Bpu10I    BsaHI    BseRI Bsp1286I
    BstXI     BtsI     EaeI     EarI  Eco57MI EcoO109I     MmeI     MscI
   MspA1I     PstI    PvuII     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI