(Linear) (Six-base) MAPSORT of: KIEE0226.seq  Check: 9970  from: 1  to: 384



KIEE0226.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     384 
   Size:         55     329
 
ApaLI G'TGCA_C

Cuts at:      0      91     384 
   Size:         91     293
 
AscI GG'CGCG_CC

Cuts at:      0     140     384 
   Size:        140     244
 
AvaI C'yCGr_G

Cuts at:      0      59     384 
   Size:         59     325
 
BanII G_rGCy'C

Cuts at:      0     281     384 
   Size:        281     103
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     232     364     384 
   Size:        232     132      20

  Fragments arranged by size: 

                 232     132      20
 
Bme1580I G_kGCm'C

Cuts at:      0      95     384 
   Size:         95     289
 
BmgBI CAC'GTC

Cuts at:      0      10     384 
   Size:         10     374
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     211     384 
   Size:        187      24     173

  Fragments arranged by size: 

                 187     173      24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     384 
   Size:        289      95
 
BseYI C'CCAG_C

Cuts at:      0      64     291     384 
   Size:         64     227      93

  Fragments arranged by size: 

                 227      93      64
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     281     384 
   Size:         95     186     103

  Fragments arranged by size: 

                 186     103      95
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     281     384 
   Size:         95     186     103

  Fragments arranged by size: 

                 186     103      95
 
BsrBI CCG'CTC

Cuts at:      0      53     126     384 
   Size:         53      73     258

  Fragments arranged by size: 

                 258      73      53
 
BsrDI GCAATG_nn'

Cuts at:      0     210     384 
   Size:        210     174
 
BsrFI r'CCGG_y

Cuts at:      0     136     384 
   Size:        136     248
 
BssHII G'CGCG_C

Cuts at:      0     140     384 
   Size:        140     244
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     384 
   Size:         99     285
 
BstYI r'GATC_y

Cuts at:      0     185     384 
   Size:        185     199
 
BtgI C'CryG_G

Cuts at:      0      80     384 
   Size:         80     304
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     291     384 
   Size:        291      93
 
EaeI y'GGCC_r

Cuts at:      0     257     384 
   Size:        257     127
 
EarI CTCTTCn'nnn_

Cuts at:      0      60     384 
   Size:         60     324
 
EcoICRI GAG'CTC

Cuts at:      0     279     384 
   Size:        279     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     187     211     384 
   Size:         55     132      24     173

  Fragments arranged by size: 

                 173     132      55      24
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     276     384 
   Size:        111     165     108

  Fragments arranged by size: 

                 165     111     108
 
MscI TGG'CCA

Cuts at:      0     259     384 
   Size:        259     125
 
MspA1I CmG'CkG

Cuts at:      0     181     384 
   Size:        181     203
 
NaeI GCC'GGC

Cuts at:      0     138     384 
   Size:        138     246
 
NgoMIV G'CCGG_C

Cuts at:      0     136     384 
   Size:        136     248
 
PfoI T'CCnGG_A

Cuts at:      0     188     384 
   Size:        188     196
 
SacI G_AGCT'C

Cuts at:      0     281     384 
   Size:        281     103
 
SapI GCTCTTCn'nnn_

Cuts at:      0      60     384 
   Size:         60     324
 
StyI C'CwwG_G

Cuts at:      0      38     384 
   Size:         38     346
 

 Enzymes that do cut:

     AcuI    ApaLI     AscI     AvaI    BanII     BbsI Bme1580I    BmgBI
     BpmI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrBI    BsrDI    BsrFI
   BssHII   BstAPI    BstYI     BtgI    BtgZI     EaeI     EarI  EcoICRI
  Eco57MI     MmeI     MscI   MspA1I     NaeI   NgoMIV     PfoI     SacI
     SapI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI