(Linear) (Six-base) MAPSORT of: KIEE0227.seq  Check: 3468  from: 1  to: 330



KIEE0227.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AleI CACnn'nnGTG

Cuts at:      0      78     330 
   Size:         78     252
 
BanI G'GyrC_C

Cuts at:      0     248     254     330 
   Size:        248       6      76

  Fragments arranged by size: 

                 248      76       6
 
BanII G_rGCy'C

Cuts at:      0     265     298     330 
   Size:        265      33      32

  Fragments arranged by size: 

                 265      33      32
 
BbeI G_GCGC'C

Cuts at:      0     252     330 
   Size:        252      78
 
Bme1580I G_kGCm'C

Cuts at:      0     109     330 
   Size:        109     221
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      96     330 
   Size:         96     234
 
Bpu10I CC'TnA_GC

Cuts at:      0     217     330 
   Size:        217     113
 
BsaHI Gr'CG_yC

Cuts at:      0     249     330 
   Size:        249      81
 
BseYI C'CCAG_C

Cuts at:      0     108     172     330 
   Size:        108      64     158

  Fragments arranged by size: 

                 158     108      64
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     241     330 
   Size:        241      89
 
Bsp1286I G_dGCh'C

Cuts at:      0     109     265     298     330 
   Size:        109     156      33      32

  Fragments arranged by size: 

                 156     109      33      32
 
BsrFI r'CCGG_y

Cuts at:      0     245     251     330 
   Size:        245       6      79

  Fragments arranged by size: 

                 245      79       6
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     316     330 
   Size:        316      14
 
BtsI GCAGTG_nn'

Cuts at:      0      31     330 
   Size:         31     299
 
EarI CTCTTCn'nnn_

Cuts at:      0     138     244     330 
   Size:        138     106      86

  Fragments arranged by size: 

                 138     106      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0     230     330 
   Size:        230     100
 
HaeII r_GCGC'y

Cuts at:      0     252     330 
   Size:        252      78
 
KasI G'GCGC_C

Cuts at:      0     248     330 
   Size:        248      82
 
MslI CAynn'nnrTG

Cuts at:      0      78     330 
   Size:         78     252
 
NaeI GCC'GGC

Cuts at:      0     253     330 
   Size:        253      77
 
NarI GG'CG_CC

Cuts at:      0     249     330 
   Size:        249      81
 
NgoMIV G'CCGG_C

Cuts at:      0     251     330 
   Size:        251      79
 
PstI C_TGCA'G

Cuts at:      0     129     330 
   Size:        129     201
 
SfcI C'TryA_G

Cuts at:      0     125     330 
   Size:        125     205
 
SfoI GGC'GCC

Cuts at:      0     250     330 
   Size:        250      80
 
SgrAI Cr'CCGG_yG

Cuts at:      0     245     330 
   Size:        245      85
 

 Enzymes that do cut:

     AleI     BanI    BanII     BbeI Bme1580I     BmrI   Bpu10I    BsaHI
    BseYI    BsmBI Bsp1286I    BsrFI    BstXI     BtsI     EarI EcoO109I
    HaeII     KasI     MslI     NaeI     NarI   NgoMIV     PstI     SfcI
     SfoI    SgrAI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI