(Linear) (Six-base) MAPSORT of: KIEE0229.seq  Check: 8337  from: 1  to: 912



KIEE0229.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     912 
   Size:        670     242
 
ApoI r'AATT_y

Cuts at:      0     191     277     496     508     680     850     912 
   Size:        191      86     219      12     172     170      62

  Fragments arranged by size: 

                 219     191     172     170      86      62      12
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     637     670     912 
   Size:        637      33     242

  Fragments arranged by size: 

                 637     242      33
 
BamHI G'GATC_C

Cuts at:      0     587     912 
   Size:        587     325
 
BanII G_rGCy'C

Cuts at:      0     243     912 
   Size:        243     669
 
BfrBI ATG'CAT

Cuts at:      0     186     912 
   Size:        186     726
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     830     912 
   Size:        830      82
 
BsaWI w'CCGG_w

Cuts at:      0     517     584     912 
   Size:        517      67     328

  Fragments arranged by size: 

                 517     328      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     766     796     912 
   Size:        766      30     116

  Fragments arranged by size: 

                 766     116      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     243     912 
   Size:        243     669
 
BspEI T'CCGG_A

Cuts at:      0     517     912 
   Size:        517     395
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     380     768     912 
   Size:        380     388     144

  Fragments arranged by size: 

                 388     380     144
 
BstYI r'GATC_y

Cuts at:      0     587     912 
   Size:        587     325
 
EaeI y'GGCC_r

Cuts at:      0     410     912 
   Size:        410     502
 
EarI CTCTTCn'nnn_

Cuts at:      0     604     912 
   Size:        604     308
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     830     912 
   Size:        670     160      82

  Fragments arranged by size: 

                 670     160      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0     536     912 
   Size:        536     376
 
MscI TGG'CCA

Cuts at:      0     412     912 
   Size:        412     500
 
NsiI A_TGCA'T

Cuts at:      0     188     912 
   Size:        188     724
 
NspI r_CATG'y

Cuts at:      0     582     912 
   Size:        582     330
 
PfoI T'CCnGG_A

Cuts at:      0     656     912 
   Size:        656     256
 
PpuMI rG'GwC_Cy

Cuts at:      0     536     912 
   Size:        536     376
 
StyI C'CwwG_G

Cuts at:      0     422     912 
   Size:        422     490
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     766     798     912 
   Size:        766      32     114

  Fragments arranged by size: 

                 766     114      32
 
XbaI T'CTAG_A

Cuts at:      0     249     912 
   Size:        249     663
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     377     429     765     912 
   Size:        377      52     336     147

  Fragments arranged by size: 

                 377     336     147      52
 

 Enzymes that do cut:

     AcuI     ApoI     BaeI    BamHI    BanII    BfrBI     BpmI    BsaWI
    BsaXI Bsp1286I    BspEI    BstXI    BstYI     EaeI     EarI  Eco57MI
 EcoO109I     MscI     NsiI     NspI     PfoI    PpuMI     StyI     TstI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XhoI     XmaI
     XmnI     ZraI