(Linear) (Six-base) MAPSORT of: KIEE0230.seq Check: 6587 from: 1 to: 531 KIEE0230.orf With 182 enzymes: SgfI * April 24, 2009 13:12 .. AlwNI CAG_nnn'CTG Cuts at: 0 26 410 440 470 531 Size: 26 384 30 30 61 Fragments arranged by size: 384 61 30 30 26 BanI G'GyrC_C Cuts at: 0 207 531 Size: 207 324 BanII G_rGCy'C Cuts at: 0 250 280 531 Size: 250 30 251 Fragments arranged by size: 251 250 30 BglI GCCn_nnn'nGGC Cuts at: 0 107 531 Size: 107 424 BmrI ACTGGGnnnn_n' Cuts at: 0 336 531 Size: 336 195 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3 39 123 144 531 Size: 3 36 84 21 387 Fragments arranged by size: 387 84 36 21 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 363 531 Size: 363 168 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 531 Size: 207 324 BsiEI CG_ry'CG Cuts at: 0 102 531 Size: 102 429 BsiHKAI G_wGCw'C Cuts at: 0 202 531 Size: 202 329 Bsp1286I G_dGCh'C Cuts at: 0 202 250 280 531 Size: 202 48 30 251 Fragments arranged by size: 251 202 48 30 BstAPI GCAn_nnn'nTGC Cuts at: 0 419 531 Size: 419 112 Bsu36I CC'TnA_GG Cuts at: 0 375 531 Size: 375 156 BtgI C'CryG_G Cuts at: 0 102 531 Size: 102 429 EaeI y'GGCC_r Cuts at: 0 99 531 Size: 99 432 EagI C'GGCC_G Cuts at: 0 99 531 Size: 99 432 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3 39 123 144 531 Size: 3 36 84 21 387 Fragments arranged by size: 387 84 36 21 3 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 518 531 Size: 518 13 MspA1I CmG'CkG Cuts at: 0 59 143 230 350 407 437 467 531 Size: 59 84 87 120 57 30 30 64 Fragments arranged by size: 120 87 84 64 59 57 30 30 PstI C_TGCA'G Cuts at: 0 136 531 Size: 136 395 PvuII CAG'CTG Cuts at: 0 59 143 230 350 407 437 467 531 Size: 59 84 87 120 57 30 30 64 Fragments arranged by size: 120 87 84 64 59 57 30 30 SfcI C'TryA_G Cuts at: 0 132 531 Size: 132 399 StyI C'CwwG_G Cuts at: 0 251 281 311 531 Size: 251 30 30 220 Fragments arranged by size: 251 220 30 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 195 531 Size: 195 336 Enzymes that do cut: AlwNI BanI BanII BglI BmrI BpmI BseRI BsgI BsiEI BsiHKAI Bsp1286I BstAPI Bsu36I BtgI EaeI EagI Eco57MI MmeI MspA1I PstI PvuII SfcI StyI TaqII Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI