(Linear) (Six-base) MAPSORT of: KIEE0230.seq  Check: 6587  from: 1  to: 531



KIEE0230.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      26     410     440     470     531 
   Size:         26     384      30      30      61

  Fragments arranged by size: 

                 384      61      30      30      26
 
BanI G'GyrC_C

Cuts at:      0     207     531 
   Size:        207     324
 
BanII G_rGCy'C

Cuts at:      0     250     280     531 
   Size:        250      30     251

  Fragments arranged by size: 

                 251     250      30
 
BglI GCCn_nnn'nGGC

Cuts at:      0     107     531 
   Size:        107     424
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     336     531 
   Size:        336     195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3      39     123     144     531 
   Size:          3      36      84      21     387

  Fragments arranged by size: 

                 387      84      36      21       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     363     531 
   Size:        363     168
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     531 
   Size:        207     324
 
BsiEI CG_ry'CG

Cuts at:      0     102     531 
   Size:        102     429
 
BsiHKAI G_wGCw'C

Cuts at:      0     202     531 
   Size:        202     329
 
Bsp1286I G_dGCh'C

Cuts at:      0     202     250     280     531 
   Size:        202      48      30     251

  Fragments arranged by size: 

                 251     202      48      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     419     531 
   Size:        419     112
 
Bsu36I CC'TnA_GG

Cuts at:      0     375     531 
   Size:        375     156
 
BtgI C'CryG_G

Cuts at:      0     102     531 
   Size:        102     429
 
EaeI y'GGCC_r

Cuts at:      0      99     531 
   Size:         99     432
 
EagI C'GGCC_G

Cuts at:      0      99     531 
   Size:         99     432
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3      39     123     144     531 
   Size:          3      36      84      21     387

  Fragments arranged by size: 

                 387      84      36      21       3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     531 
   Size:        518      13
 
MspA1I CmG'CkG

Cuts at:      0      59     143     230     350     407     437     467     531 
   Size:         59      84      87     120      57      30      30      64

  Fragments arranged by size: 

                 120      87      84      64      59      57      30      30
            
 
PstI C_TGCA'G

Cuts at:      0     136     531 
   Size:        136     395
 
PvuII CAG'CTG

Cuts at:      0      59     143     230     350     407     437     467     531 
   Size:         59      84      87     120      57      30      30      64

  Fragments arranged by size: 

                 120      87      84      64      59      57      30      30
            
 
SfcI C'TryA_G

Cuts at:      0     132     531 
   Size:        132     399
 
StyI C'CwwG_G

Cuts at:      0     251     281     311     531 
   Size:        251      30      30     220

  Fragments arranged by size: 

                 251     220      30      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     195     531 
   Size:        195     336
 

 Enzymes that do cut:

    AlwNI     BanI    BanII     BglI     BmrI     BpmI    BseRI     BsgI
    BsiEI  BsiHKAI Bsp1286I   BstAPI   Bsu36I     BtgI     EaeI     EagI
  Eco57MI     MmeI   MspA1I     PstI    PvuII     SfcI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI