Restriction map for
pF1KE0232
(Linear) (Six-base) MAPSORT of: KIEE0232.seq Check: 6948 from: 1 to: 936
KIEE0232.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 68 936
Size: 68 868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 936
Size: 691 245
AflIII A'CryG_T
Cuts at: 0 98 936
Size: 98 838
AleI CACnn'nnGTG
Cuts at: 0 142 936
Size: 142 794
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 936
Size: 803 32 101
Fragments arranged by size:
803 101 32
ApoI r'AATT_y
Cuts at: 0 186 325 493 936
Size: 186 139 168 443
Fragments arranged by size:
443 186 168 139
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 557 590 936
Size: 557 33 346
Fragments arranged by size:
557 346 33
BclI T'GATC_A
Cuts at: 0 455 516 936
Size: 455 61 420
Fragments arranged by size:
455 420 61
BglII A'GATC_T
Cuts at: 0 551 936
Size: 551 385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 936
Size: 469 467
Bpu10I CC'TnA_GC
Cuts at: 0 477 936
Size: 477 459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 936
Size: 888 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 803 833 936
Size: 803 30 103
Fragments arranged by size:
803 103 30
BseYI C'CCAG_C
Cuts at: 0 36 936
Size: 36 900
BsmI GAATG_Cn'
Cuts at: 0 224 936
Size: 224 712
BspHI T'CATG_A
Cuts at: 0 398 936
Size: 398 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 526 936
Size: 68 458 410
Fragments arranged by size:
458 410 68
BsrDI GCAATG_nn'
Cuts at: 0 740 936
Size: 740 196
BsrGI T'GTAC_A
Cuts at: 0 239 650 936
Size: 239 411 286
Fragments arranged by size:
411 286 239
BstAPI GCAn_nnn'nTGC
Cuts at: 0 69 936
Size: 69 867
BstEII G'GTnAC_C
Cuts at: 0 49 936
Size: 49 887
BstYI r'GATC_y
Cuts at: 0 551 936
Size: 551 385
EaeI y'GGCC_r
Cuts at: 0 365 797 936
Size: 365 432 139
Fragments arranged by size:
432 365 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 691 936
Size: 469 222 245
Fragments arranged by size:
469 245 222
EcoO109I rG'GnC_Cy
Cuts at: 0 538 936
Size: 538 398
EcoRI G'AATT_C
Cuts at: 0 493 936
Size: 493 443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 88 936
Size: 88 848
HindIII A'AGCT_T
Cuts at: 0 890 936
Size: 890 46
MscI TGG'CCA
Cuts at: 0 367 799 936
Size: 367 432 137
Fragments arranged by size:
432 367 137
MslI CAynn'nnrTG
Cuts at: 0 97 142 730 936
Size: 97 45 588 206
Fragments arranged by size:
588 206 97 45
NspI r_CATG'y
Cuts at: 0 102 936
Size: 102 834
PciI A'CATG_T
Cuts at: 0 98 936
Size: 98 838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 803 835 936
Size: 803 32 101
Fragments arranged by size:
803 101 32
PpuMI rG'GwC_Cy
Cuts at: 0 538 936
Size: 538 398
SfcI C'TryA_G
Cuts at: 0 215 936
Size: 215 721
SmlI C'TyrA_G
Cuts at: 0 867 936
Size: 867 69
SspI AAT'ATT
Cuts at: 0 296 936
Size: 296 640
StuI AGG'CCT
Cuts at: 0 482 703 936
Size: 482 221 233
Fragments arranged by size:
482 233 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 429 936
Size: 429 507
TatI w'GTAC_w
Cuts at: 0 170 239 650 936
Size: 170 69 411 286
Fragments arranged by size:
411 286 170 69
XmnI GAAnn'nnTTC
Cuts at: 0 189 329 853 936
Size: 189 140 524 83
Fragments arranged by size:
524 189 140 83
Enzymes that do cut:
AarI AcuI AflIII AleI AloI ApoI BaeI BclI
BglII BpmI Bpu10I BpuEI BsaXI BseYI BsmI BspHI
BspMI BsrDI BsrGI BstAPI BstEII BstYI EaeI Eco57MI
EcoO109I EcoRI FalI HindIII MscI MslI NspI PciI
PpiI PpuMI SfcI SmlI SspI StuI TaqII TatI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrFI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StyI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI