Restriction map for
pF1KE0234
(Linear) (Six-base) MAPSORT of: KIEE0234.seq Check: 546 from: 1 to: 762
KIEE0234.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 623 762
Size: 48 575 139
Fragments arranged by size:
575 139 48
AflIII A'CryG_T
Cuts at: 0 537 762
Size: 537 225
AgeI A'CCGG_T
Cuts at: 0 286 762
Size: 286 476
AleI CACnn'nnGTG
Cuts at: 0 172 762
Size: 172 590
AlwNI CAG_nnn'CTG
Cuts at: 0 71 307 696 762
Size: 71 236 389 66
Fragments arranged by size:
389 236 71 66
BanI G'GyrC_C
Cuts at: 0 254 762
Size: 254 508
BanII G_rGCy'C
Cuts at: 0 94 383 489 762
Size: 94 289 106 273
Fragments arranged by size:
289 273 106 94
BbeI G_GCGC'C
Cuts at: 0 258 762
Size: 258 504
BciVI GTATCCnnnnn_n'
Cuts at: 0 465 762
Size: 465 297
BclI T'GATC_A
Cuts at: 0 222 762
Size: 222 540
BglI GCCn_nnn'nGGC
Cuts at: 0 98 572 762
Size: 98 474 190
Fragments arranged by size:
474 190 98
BglII A'GATC_T
Cuts at: 0 338 762
Size: 338 424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 615 762
Size: 615 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 363 553 762
Size: 73 290 190 209
Fragments arranged by size:
290 209 190 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 601 762
Size: 295 306 161
Fragments arranged by size:
306 295 161
BsaHI Gr'CG_yC
Cuts at: 0 80 255 762
Size: 80 175 507
Fragments arranged by size:
507 175 80
BsaWI w'CCGG_w
Cuts at: 0 191 286 762
Size: 191 95 476
Fragments arranged by size:
476 191 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 762
Size: 121 641
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 762
Size: 196 566
BsiHKAI G_wGCw'C
Cuts at: 0 43 489 762
Size: 43 446 273
Fragments arranged by size:
446 273 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 703 762
Size: 703 59
Bsp1286I G_dGCh'C
Cuts at: 0 43 94 383 489 762
Size: 43 51 289 106 273
Fragments arranged by size:
289 273 106 51 43
BspEI T'CCGG_A
Cuts at: 0 191 762
Size: 191 571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 410 635 762
Size: 410 225 127
Fragments arranged by size:
410 225 127
BsrBI CCG'CTC
Cuts at: 0 267 762
Size: 267 495
BsrDI GCAATG_nn'
Cuts at: 0 575 762
Size: 575 187
BsrFI r'CCGG_y
Cuts at: 0 286 762
Size: 286 476
BssSI C'ACGA_G
Cuts at: 0 103 762
Size: 103 659
BstAPI GCAn_nnn'nTGC
Cuts at: 0 307 762
Size: 307 455
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 762
Size: 588 174
BstYI r'GATC_y
Cuts at: 0 338 762
Size: 338 424
BtgI C'CryG_G
Cuts at: 0 555 762
Size: 555 207
EaeI y'GGCC_r
Cuts at: 0 2 762
Size: 2 760
EarI CTCTTCn'nnn_
Cuts at: 0 175 599 701 762
Size: 175 424 102 61
Fragments arranged by size:
424 175 102 61
EcoICRI GAG'CTC
Cuts at: 0 487 762
Size: 487 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 73 363 553 623 762
Size: 48 25 290 190 70 139
Fragments arranged by size:
290 190 139 70 48 25
HaeII r_GCGC'y
Cuts at: 0 133 258 351 762
Size: 133 125 93 411
Fragments arranged by size:
411 133 125 93
KasI G'GCGC_C
Cuts at: 0 254 762
Size: 254 508
MslI CAynn'nnrTG
Cuts at: 0 172 762
Size: 172 590
MspA1I CmG'CkG
Cuts at: 0 68 762
Size: 68 694
NarI GG'CG_CC
Cuts at: 0 255 762
Size: 255 507
NcoI C'CATG_G
Cuts at: 0 555 762
Size: 555 207
NspI r_CATG'y
Cuts at: 0 589 762
Size: 589 173
PasI CC'CwG_GG
Cuts at: 0 744 762
Size: 744 18
PvuII CAG'CTG
Cuts at: 0 68 762
Size: 68 694
SacI G_AGCT'C
Cuts at: 0 489 762
Size: 489 273
SfcI C'TryA_G
Cuts at: 0 396 762
Size: 396 366
SfoI GGC'GCC
Cuts at: 0 256 762
Size: 256 506
StyI C'CwwG_G
Cuts at: 0 555 762
Size: 555 207
TatI w'GTAC_w
Cuts at: 0 540 762
Size: 540 222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 693 732 762
Size: 693 39 30
Fragments arranged by size:
693 39 30
XmnI GAAnn'nnTTC
Cuts at: 0 61 512 762
Size: 61 451 250
Fragments arranged by size:
451 250 61
Enzymes that do cut:
AcuI AflIII AgeI AleI AlwNI BanI BanII BbeI
BciVI BclI BglI BglII BplI BpmI BsaI BsaHI
BsaWI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI
EaeI EarI EcoICRI Eco57MI HaeII KasI MslI MspA1I
NarI NcoI NspI PasI PvuII SacI SfcI SfoI
StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BfrBI
BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaAI
BsaBI BsaXI BseYI BsiEI BsiWI BsmI BspHI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI