Restriction map for
pF1KE0241
(Linear) (Six-base) MAPSORT of: KIEE0241.seq Check: 6815 from: 1 to: 1281
KIEE0241.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
Acc65I G'GTAC_C
Cuts at: 0 200 1281
Size: 200 1081
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 567 734 1281
Size: 23 544 167 547
Fragments arranged by size:
547 544 167 23
AflIII A'CryG_T
Cuts at: 0 223 1281
Size: 223 1058
AleI CACnn'nnGTG
Cuts at: 0 226 1281
Size: 226 1055
ApoI r'AATT_y
Cuts at: 0 1113 1281
Size: 1113 168
BanI G'GyrC_C
Cuts at: 0 200 1281
Size: 200 1081
BanII G_rGCy'C
Cuts at: 0 1253 1281
Size: 1253 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 573 830 1166 1281
Size: 53 520 257 336 115
Fragments arranged by size:
520 336 257 115 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 107 141 1281
Size: 107 34 1140
Fragments arranged by size:
1140 107 34
BfrBI ATG'CAT
Cuts at: 0 343 1281
Size: 343 938
BglI GCCn_nnn'nGGC
Cuts at: 0 70 547 1263 1281
Size: 70 477 716 18
Fragments arranged by size:
716 477 70 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 706 1281
Size: 706 575
BsaI GGTCTCn'nnnn_
Cuts at: 0 406 1281
Size: 406 875
BsaAI yAC'GTr
Cuts at: 0 224 1281
Size: 224 1057
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 1281
Size: 682 599
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1281
Size: 147 1134
BsiEI CG_ry'CG
Cuts at: 0 777 1281
Size: 777 504
BsiHKAI G_wGCw'C
Cuts at: 0 687 989 1281
Size: 687 302 292
Fragments arranged by size:
687 302 292
BsmI GAATG_Cn'
Cuts at: 0 463 1281
Size: 463 818
BsmBI CGTCTCn'nnnn_
Cuts at: 0 39 605 1281
Size: 39 566 676
Fragments arranged by size:
676 566 39
Bsp1286I G_dGCh'C
Cuts at: 0 687 989 1253 1281
Size: 687 302 264 28
Fragments arranged by size:
687 302 264 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 1281
Size: 228 1053
BtgI C'CryG_G
Cuts at: 0 68 327 1281
Size: 68 259 954
Fragments arranged by size:
954 259 68
BtsI GCAGTG_nn'
Cuts at: 0 12 911 1281
Size: 12 899 370
Fragments arranged by size:
899 370 12
ClaI AT'CG_AT
Cuts at: 0 512 773 1281
Size: 512 261 508
Fragments arranged by size:
512 508 261
DraIII CAC_nnn'GTG
Cuts at: 0 224 666 1281
Size: 224 442 615
Fragments arranged by size:
615 442 224
EaeI y'GGCC_r
Cuts at: 0 62 153 539 1281
Size: 62 91 386 742
Fragments arranged by size:
742 386 91 62
EarI CTCTTCn'nnn_
Cuts at: 0 42 94 406 556 989 1281
Size: 42 52 312 150 433 292
Fragments arranged by size:
433 312 292 150 52 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 567 734 1281
Size: 23 544 167 547
Fragments arranged by size:
547 544 167 23
EcoO109I rG'GnC_Cy
Cuts at: 0 333 1256 1281
Size: 333 923 25
Fragments arranged by size:
923 333 25
HincII GTy'rAC
Cuts at: 0 378 1281
Size: 378 903
HindIII A'AGCT_T
Cuts at: 0 359 1281
Size: 359 922
KpnI G_GTAC'C
Cuts at: 0 204 1281
Size: 204 1077
MfeI C'AATT_G
Cuts at: 0 464 1281
Size: 464 817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 1281
Size: 1253 28
MscI TGG'CCA
Cuts at: 0 64 155 541 1281
Size: 64 91 386 740
Fragments arranged by size:
740 386 91 64
MslI CAynn'nnrTG
Cuts at: 0 161 178 226 481 916 974 1281
Size: 161 17 48 255 435 58 307
Fragments arranged by size:
435 307 255 161 58 48 17
NcoI C'CATG_G
Cuts at: 0 68 327 1281
Size: 68 259 954
Fragments arranged by size:
954 259 68
NsiI A_TGCA'T
Cuts at: 0 345 1281
Size: 345 936
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 1281
Size: 666 615
PmlI CAC'GTG
Cuts at: 0 224 1281
Size: 224 1057
PpuMI rG'GwC_Cy
Cuts at: 0 333 1281
Size: 333 948
PshAI GACnn'nnGTC
Cuts at: 0 577 1281
Size: 577 704
PvuI CG_AT'CG
Cuts at: 0 777 1281
Size: 777 504
SfcI C'TryA_G
Cuts at: 0 24 803 1281
Size: 24 779 478
Fragments arranged by size:
779 478 24
SpeI A'CTAG_T
Cuts at: 0 1192 1281
Size: 1192 89
StyI C'CwwG_G
Cuts at: 0 68 327 1281
Size: 68 259 954
Fragments arranged by size:
954 259 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 1281
Size: 172 1109
XbaI T'CTAG_A
Cuts at: 0 1245 1281
Size: 1245 36
XmnI GAAnn'nnTTC
Cuts at: 0 708 1281
Size: 708 573
Enzymes that do cut:
Acc65I AcuI AflIII AleI ApoI BanI BanII BbsI
BcgI BfrBI BglI BplI BsaI BsaAI BseRI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BstXI BtgI BtsI
ClaI DraIII EaeI EarI Eco57MI EcoO109I HincII HindIII
KpnI MfeI MmeI MscI MslI NcoI NsiI PflMI
PmlI PpuMI PshAI PvuI SfcI SpeI StyI TaqII
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsiWI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HpaI KasI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PpiI
PsiI PspOMI PspXI PsrI PstI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XcmI XhoI
XmaI ZraI