Restriction map for
pF1KE0243
(Linear) (Six-base) MAPSORT of: KIEE0243.seq Check: 559 from: 1 to: 1164
KIEE0243.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 0 314 644 1164
Size: 314 330 520
Fragments arranged by size:
520 330 314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 145 1164
Size: 133 12 1019
Fragments arranged by size:
1019 133 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 859 1164
Size: 859 305
AlwNI CAG_nnn'CTG
Cuts at: 0 201 618 1164
Size: 201 417 546
Fragments arranged by size:
546 417 201
BamHI G'GATC_C
Cuts at: 0 1082 1164
Size: 1082 82
BanI G'GyrC_C
Cuts at: 0 303 412 729 835 1164
Size: 303 109 317 106 329
Fragments arranged by size:
329 317 303 109 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 954 1089 1113 1164
Size: 954 135 24 51
Fragments arranged by size:
954 135 51 24
BbvCI CC'TCA_GC
Cuts at: 0 649 806 1164
Size: 649 157 358
Fragments arranged by size:
649 358 157
BciVI GTATCCnnnnn_n'
Cuts at: 0 433 1164
Size: 433 731
BglI GCCn_nnn'nGGC
Cuts at: 0 1159 1164
Size: 1159 5
BglII A'GATC_T
Cuts at: 0 482 1164
Size: 482 682
BlpI GC'TnA_GC
Cuts at: 0 933 1164
Size: 933 231
Bme1580I G_kGCm'C
Cuts at: 0 308 1164
Size: 308 856
BmrI ACTGGGnnnn_n'
Cuts at: 0 159 735 992 1164
Size: 159 576 257 172
Fragments arranged by size:
576 257 172 159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 414 1164
Size: 414 750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 306 1091 1164
Size: 223 83 785 73
Fragments arranged by size:
785 223 83 73
Bpu10I CC'TnA_GC
Cuts at: 0 493 649 806 1164
Size: 493 156 157 358
Fragments arranged by size:
493 358 157 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 1164
Size: 102 1062
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 271 301 1164
Size: 271 30 863
Fragments arranged by size:
863 271 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 279 564 1164
Size: 279 285 600
Fragments arranged by size:
600 285 279
BseYI C'CCAG_C
Cuts at: 0 18 144 562 1164
Size: 18 126 418 602
Fragments arranged by size:
602 418 126 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1164
Size: 318 846
BsmBI CGTCTCn'nnnn_
Cuts at: 0 493 1164
Size: 493 671
Bsp1286I G_dGCh'C
Cuts at: 0 308 1164
Size: 308 856
BsrDI GCAATG_nn'
Cuts at: 0 866 1164
Size: 866 298
BsrFI r'CCGG_y
Cuts at: 0 283 415 832 850 1164
Size: 283 132 417 18 314
Fragments arranged by size:
417 314 283 132 18
BsrGI T'GTAC_A
Cuts at: 0 50 1164
Size: 50 1114
BstYI r'GATC_y
Cuts at: 0 482 1082 1164
Size: 482 600 82
Fragments arranged by size:
600 482 82
Bsu36I CC'TnA_GG
Cuts at: 0 359 1164
Size: 359 805
BtgI C'CryG_G
Cuts at: 0 764 1157 1164
Size: 764 393 7
Fragments arranged by size:
764 393 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 780 1164
Size: 780 384
DraIII CAC_nnn'GTG
Cuts at: 0 1000 1164
Size: 1000 164
DrdI GACnn_nn'nnGTC
Cuts at: 0 922 1164
Size: 922 242
EarI CTCTTCn'nnn_
Cuts at: 0 640 705 1164
Size: 640 65 459
Fragments arranged by size:
640 459 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1093 1164
Size: 1093 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 145 223 306 1091 1164
Size: 133 12 78 83 785 73
Fragments arranged by size:
785 133 83 78 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 112 1164
Size: 112 1052
EcoO109I rG'GnC_Cy
Cuts at: 0 57 615 630 1164
Size: 57 558 15 534
Fragments arranged by size:
558 534 57 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 96 1164
Size: 96 1068
HaeII r_GCGC'y
Cuts at: 0 34 656 1164
Size: 34 622 508
Fragments arranged by size:
622 508 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 622 654 779 811 1164
Size: 271 32 319 32 125 32 353
Fragments arranged by size:
353 319 271 125 32 32 32
HincII GTy'rAC
Cuts at: 0 828 1164
Size: 828 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 438 1164
Size: 317 121 726
Fragments arranged by size:
726 317 121
MslI CAynn'nnrTG
Cuts at: 0 778 1164
Size: 778 386
MspA1I CmG'CkG
Cuts at: 0 929 1164
Size: 929 235
PflMI CCAn_nnn'nTGG
Cuts at: 0 299 618 1164
Size: 299 319 546
Fragments arranged by size:
546 319 299
PpuMI rG'GwC_Cy
Cuts at: 0 57 630 1164
Size: 57 573 534
Fragments arranged by size:
573 534 57
PstI C_TGCA'G
Cuts at: 0 1016 1164
Size: 1016 148
PvuII CAG'CTG
Cuts at: 0 929 1164
Size: 929 235
ScaI AGT'ACT
Cuts at: 0 157 226 1111 1164
Size: 157 69 885 53
Fragments arranged by size:
885 157 69 53
SexAI A'CCwGG_T
Cuts at: 0 1139 1164
Size: 1139 25
SfcI C'TryA_G
Cuts at: 0 1012 1164
Size: 1012 152
SmlI C'TyrA_G
Cuts at: 0 81 1164
Size: 81 1083
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 291 717 1011 1078 1164
Size: 291 426 294 67 86
Fragments arranged by size:
426 294 291 86 67
TatI w'GTAC_w
Cuts at: 0 50 155 224 1109 1164
Size: 50 105 69 885 55
Fragments arranged by size:
885 105 69 55 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1074 1106 1164
Size: 1074 32 58
Fragments arranged by size:
1074 58 32
Tth111I GACn'n_nGTC
Cuts at: 0 448 958 1164
Size: 448 510 206
Fragments arranged by size:
510 448 206
XmnI GAAnn'nnTTC
Cuts at: 0 118 1164
Size: 118 1046
Enzymes that do cut:
AccI AcuI AlfI AlwNI BamHI BanI BbsI BbvCI
BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I
BsrDI BsrFI BsrGI BstYI Bsu36I BtgI BtgZI DraIII
DrdI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII MmeI MslI MspA1I PflMI PpuMI PstI
PvuII ScaI SexAI SfcI SmlI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanII BbeI BcgI BclI
BfrBI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtsI
ClaI CspCI DraI EaeI EagI EcoICRI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI XbaI XcmI XhoI
XmaI ZraI