(Linear) (Six-base) MAPSORT of: KIEE0244.seq Check: 8909 from: 1 to: 402 KIEE0244.orf With 182 enzymes: SgfI * April 24, 2009 13:12 .. ApoI r'AATT_y Cuts at: 0 196 402 Size: 196 206 BanII G_rGCy'C Cuts at: 0 274 402 Size: 274 128 BclI T'GATC_A Cuts at: 0 93 356 402 Size: 93 263 46 Fragments arranged by size: 263 93 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 402 Size: 286 116 BsaAI yAC'GTr Cuts at: 0 60 402 Size: 60 342 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 322 352 402 Size: 322 30 50 Fragments arranged by size: 322 50 30 BseYI C'CCAG_C Cuts at: 0 276 402 Size: 276 126 Bsp1286I G_dGCh'C Cuts at: 0 274 402 Size: 274 128 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 61 402 Size: 61 341 BsrGI T'GTAC_A Cuts at: 0 291 402 Size: 291 111 BstBI TT'CG_AA Cuts at: 0 24 402 Size: 24 378 PshAI GACnn'nnGTC Cuts at: 0 148 402 Size: 148 254 SmlI C'TyrA_G Cuts at: 0 265 402 Size: 265 137 SspI AAT'ATT Cuts at: 0 210 402 Size: 210 192 StyI C'CwwG_G Cuts at: 0 341 402 Size: 341 61 TatI w'GTAC_w Cuts at: 0 291 402 Size: 291 111 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 322 354 402 Size: 322 32 48 Fragments arranged by size: 322 48 32 XbaI T'CTAG_A Cuts at: 0 397 402 Size: 397 5 Enzymes that do cut: ApoI BanII BclI BpuEI BsaAI BsaXI BseYI Bsp1286I BspMI BsrGI BstBI PshAI SmlI SspI StyI TatI TstI XbaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I XcmI XhoI XmaI XmnI ZraI