(Linear) (Six-base) MAPSORT of: KIEE0244.seq Check: 8909 from: 1 to: 402
KIEE0244.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
ApoI r'AATT_y
Cuts at: 0 196 402
Size: 196 206
BanII G_rGCy'C
Cuts at: 0 274 402
Size: 274 128
BclI T'GATC_A
Cuts at: 0 93 356 402
Size: 93 263 46
Fragments arranged by size:
263 93 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 402
Size: 286 116
BsaAI yAC'GTr
Cuts at: 0 60 402
Size: 60 342
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 352 402
Size: 322 30 50
Fragments arranged by size:
322 50 30
BseYI C'CCAG_C
Cuts at: 0 276 402
Size: 276 126
Bsp1286I G_dGCh'C
Cuts at: 0 274 402
Size: 274 128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 61 402
Size: 61 341
BsrGI T'GTAC_A
Cuts at: 0 291 402
Size: 291 111
BstBI TT'CG_AA
Cuts at: 0 24 402
Size: 24 378
PshAI GACnn'nnGTC
Cuts at: 0 148 402
Size: 148 254
SmlI C'TyrA_G
Cuts at: 0 265 402
Size: 265 137
SspI AAT'ATT
Cuts at: 0 210 402
Size: 210 192
StyI C'CwwG_G
Cuts at: 0 341 402
Size: 341 61
TatI w'GTAC_w
Cuts at: 0 291 402
Size: 291 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 322 354 402
Size: 322 32 48
Fragments arranged by size:
322 48 32
XbaI T'CTAG_A
Cuts at: 0 397 402
Size: 397 5
Enzymes that do cut:
ApoI BanII BclI BpuEI BsaAI BsaXI BseYI Bsp1286I
BspMI BsrGI BstBI PshAI SmlI SspI StyI TatI
TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XcmI XhoI XmaI
XmnI ZraI