(Linear) (Six-base) MAPSORT of: KIEE0249.seq  Check: 8448  from: 1  to: 252



KIEE0249.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

BanI G'GyrC_C

Cuts at:      0      15     156     252 
   Size:         15     141      96

  Fragments arranged by size: 

                 141      96      15
 
BbeI G_GCGC'C

Cuts at:      0      19     252 
   Size:         19     233
 
BbvCI CC'TCA_GC

Cuts at:      0     131     252 
   Size:        131     121
 
Bpu10I CC'TnA_GC

Cuts at:      0     131     252 
   Size:        131     121
 
BsaHI Gr'CG_yC

Cuts at:      0      16     252 
   Size:         16     236
 
BseYI C'CCAG_C

Cuts at:      0     170     252 
   Size:        170      82
 
BsrBI CCG'CTC

Cuts at:      0     106     252 
   Size:        106     146
 
EaeI y'GGCC_r

Cuts at:      0     121     252 
   Size:        121     131
 
EarI CTCTTCn'nnn_

Cuts at:      0      65     252 
   Size:         65     187
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     252 
   Size:         33     219
 
HaeII r_GCGC'y

Cuts at:      0      19      98     252 
   Size:         19      79     154

  Fragments arranged by size: 

                 154      79      19
 
KasI G'GCGC_C

Cuts at:      0      15     252 
   Size:         15     237
 
MspA1I CmG'CkG

Cuts at:      0     119     165     174     252 
   Size:        119      46       9      78

  Fragments arranged by size: 

                 119      78      46       9
 
NarI GG'CG_CC

Cuts at:      0      16     252 
   Size:         16     236
 
PasI CC'CwG_GG

Cuts at:      0     189     252 
   Size:        189      63
 
PpuMI rG'GwC_Cy

Cuts at:      0      33     252 
   Size:         33     219
 
SanDI GG'GwC_CC

Cuts at:      0      33     252 
   Size:         33     219
 
SapI GCTCTTCn'nnn_

Cuts at:      0      65     252 
   Size:         65     187
 
SfoI GGC'GCC

Cuts at:      0      17     252 
   Size:         17     235
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     202     252 
   Size:        202      50
 

 Enzymes that do cut:

     BanI     BbeI    BbvCI   Bpu10I    BsaHI    BseYI    BsrBI     EaeI
     EarI EcoO109I    HaeII     KasI   MspA1I     NarI     PasI    PpuMI
    SanDI     SapI     SfoI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI