Restriction map for
pF1KE0254
(Linear) (Six-base) MAPSORT of: KIEE0254.seq Check: 1891 from: 1 to: 978
KIEE0254.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 0 41 978
Size: 41 937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 978
Size: 751 227
AlwNI CAG_nnn'CTG
Cuts at: 0 526 879 978
Size: 526 353 99
Fragments arranged by size:
526 353 99
ApoI r'AATT_y
Cuts at: 0 10 105 978
Size: 10 95 873
Fragments arranged by size:
873 95 10
BanII G_rGCy'C
Cuts at: 0 806 922 978
Size: 806 116 56
Fragments arranged by size:
806 116 56
BclI T'GATC_A
Cuts at: 0 144 942 978
Size: 144 798 36
Fragments arranged by size:
798 144 36
BglII A'GATC_T
Cuts at: 0 714 978
Size: 714 264
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 978
Size: 936 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 901 978
Size: 394 507 77
Fragments arranged by size:
507 394 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 978
Size: 844 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 482 512 608 638 978
Size: 482 30 96 30 340
Fragments arranged by size:
482 340 96 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 978
Size: 397 581
BsiHKAI G_wGCw'C
Cuts at: 0 860 922 978
Size: 860 62 56
Fragments arranged by size:
860 62 56
BsmI GAATG_Cn'
Cuts at: 0 258 978
Size: 258 720
Bsp1286I G_dGCh'C
Cuts at: 0 806 860 922 978
Size: 806 54 62 56
Fragments arranged by size:
806 62 56 54
BspHI T'CATG_A
Cuts at: 0 599 978
Size: 599 379
BsrDI GCAATG_nn'
Cuts at: 0 296 960 978
Size: 296 664 18
Fragments arranged by size:
664 296 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 526 978
Size: 526 452
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 291 978
Size: 27 264 687
Fragments arranged by size:
687 264 27
BstYI r'GATC_y
Cuts at: 0 242 651 714 978
Size: 242 409 63 264
Fragments arranged by size:
409 264 242 63
BtgI C'CryG_G
Cuts at: 0 619 978
Size: 619 359
DraI TTT'AAA
Cuts at: 0 582 978
Size: 582 396
EaeI y'GGCC_r
Cuts at: 0 281 978
Size: 281 697
EarI CTCTTCn'nnn_
Cuts at: 0 106 151 978
Size: 106 45 827
Fragments arranged by size:
827 106 45
EcoICRI GAG'CTC
Cuts at: 0 920 978
Size: 920 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 751 901 978
Size: 394 357 150 77
Fragments arranged by size:
394 357 150 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 706 738 978
Size: 706 32 240
Fragments arranged by size:
706 240 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 978
Size: 447 531
MscI TGG'CCA
Cuts at: 0 283 978
Size: 283 695
MspA1I CmG'CkG
Cuts at: 0 940 978
Size: 940 38
NspI r_CATG'y
Cuts at: 0 48 978
Size: 48 930
PsiI TTA'TAA
Cuts at: 0 229 978
Size: 229 749
PstI C_TGCA'G
Cuts at: 0 251 978
Size: 251 727
PvuII CAG'CTG
Cuts at: 0 940 978
Size: 940 38
SacI G_AGCT'C
Cuts at: 0 922 978
Size: 922 56
SfcI C'TryA_G
Cuts at: 0 59 247 978
Size: 59 188 731
Fragments arranged by size:
731 188 59
SmlI C'TyrA_G
Cuts at: 0 859 978
Size: 859 119
SpeI A'CTAG_T
Cuts at: 0 819 978
Size: 819 159
SspI AAT'ATT
Cuts at: 0 701 978
Size: 701 277
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 608 640 978
Size: 608 32 338
Fragments arranged by size:
608 338 32
XmnI GAAnn'nnTTC
Cuts at: 0 391 978
Size: 391 587
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanII BclI BglII BplI
BpmI BpuEI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI
BsrDI BstAPI BstXI BstYI BtgI DraI EaeI EarI
EcoICRI Eco57MI Hin4I MmeI MscI MspA1I NspI PsiI
PstI PvuII SacI SfcI SmlI SpeI SspI TstI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI ZraI