(Linear) (Six-base) MAPSORT of: KIEE0257.seq  Check: 9506  from: 1  to: 1197



KIEE0257.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AccI GT'mk_AC

Cuts at:      0     563     704     905    1197 
   Size:        563     141     201     292

  Fragments arranged by size: 

                 563     292     201     141
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505    1197 
   Size:        505     692
 
AfeI AGC'GCT

Cuts at:      0     426    1197 
   Size:        426     771
 
AvaI C'yCGr_G

Cuts at:      0     944    1113    1197 
   Size:        944     169      84

  Fragments arranged by size: 

                 944     169      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     493    1197 
   Size:        493     704
 
BclI T'GATC_A

Cuts at:      0     167    1197 
   Size:        167    1030
 
BfrBI ATG'CAT

Cuts at:      0     921    1197 
   Size:        921     276
 
BglII A'GATC_T

Cuts at:      0     276    1197 
   Size:        276     921
 
BlpI GC'TnA_GC

Cuts at:      0     997    1197 
   Size:        997     200
 
Bme1580I G_kGCm'C

Cuts at:      0    1120    1197 
   Size:       1120      77
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1130    1172    1197 
   Size:       1130      42      25

  Fragments arranged by size: 

                1130      42      25
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107    1197 
   Size:        107    1090
 
Bpu10I CC'TnA_GC

Cuts at:      0    1058    1197 
   Size:       1058     139
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478    1197 
   Size:        478     719
 
BsiHKAI G_wGCw'C

Cuts at:      0     368    1197 
   Size:        368     829
 
Bsp1286I G_dGCh'C

Cuts at:      0     368    1120    1197 
   Size:        368     752      77

  Fragments arranged by size: 

                 752     368      77
 
BsrBI CCG'CTC

Cuts at:      0     110    1053    1197 
   Size:        110     943     144

  Fragments arranged by size: 

                 943     144     110
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     142     968    1197 
   Size:        142     826     229

  Fragments arranged by size: 

                 826     229     142
 
BstYI r'GATC_y

Cuts at:      0     276     576    1197 
   Size:        276     300     621

  Fragments arranged by size: 

                 621     300     276
 
BstZ17I GTA'TAC

Cuts at:      0     564    1197 
   Size:        564     633
 
Bsu36I CC'TnA_GG

Cuts at:      0     542    1197 
   Size:        542     655
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      56      91    1197 
   Size:         56      35    1106

  Fragments arranged by size: 

                1106      56      35
 
DraI TTT'AAA

Cuts at:      0    1023    1197 
   Size:       1023     174
 
DraIII CAC_nnn'GTG

Cuts at:      0    1166    1197 
   Size:       1166      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1063    1197 
   Size:       1063     134
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     505    1197 
   Size:        107     398     692

  Fragments arranged by size: 

                 692     398     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0     725    1197 
   Size:        725     472
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     651    1197 
   Size:        651     546
 
HaeII r_GCGC'y

Cuts at:      0     428    1197 
   Size:        428     769
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     519     551    1197 
   Size:         46      32     441      32     646

  Fragments arranged by size: 

                 646     441      46      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1005    1197 
   Size:       1005     192
 
MslI CAynn'nnrTG

Cuts at:      0     175    1132    1197 
   Size:        175     957      65

  Fragments arranged by size: 

                 957     175      65
 
NsiI A_TGCA'T

Cuts at:      0     923    1197 
   Size:        923     274
 
PfoI T'CCnGG_A

Cuts at:      0     123    1197 
   Size:        123    1074
 
PpuMI rG'GwC_Cy

Cuts at:      0     725    1197 
   Size:        725     472
 
SmaI CCC'GGG

Cuts at:      0    1115    1197 
   Size:       1115      82
 
SmlI C'TyrA_G

Cuts at:      0     457    1197 
   Size:        457     740
 
SspI AAT'ATT

Cuts at:      0     249    1197 
   Size:        249     948
 
StyI C'CwwG_G

Cuts at:      0     186    1197 
   Size:        186    1011
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1154    1197 
   Size:       1154      43
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     816    1197 
   Size:        816     381
 
XmaI C'CCGG_G

Cuts at:      0    1113    1197 
   Size:       1113      84
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AvaI     BbsI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI  BsiHKAI Bsp1286I
    BsrBI   BstAPI    BstYI  BstZ17I   Bsu36I    CspCI     DraI   DraIII
     EciI  Eco57MI EcoO109I     FalI    HaeII    Hin4I  HindIII     MslI
     NsiI     PfoI    PpuMI     SmaI     SmlI     SspI     StyI    TaqII
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BglI    BmgBI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI     BtgI
    BtgZI     BtsI     ClaI     DrdI     EaeI     EagI     EarI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmnI     ZraI