Restriction map for
pF1KE0259
(Linear) (Six-base) MAPSORT of: KIEE0259.seq Check: 8942 from: 1 to: 750
KIEE0259.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 162 750
Size: 162 588
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 448 495 750
Size: 25 423 47 255
Fragments arranged by size:
423 255 47 25
AleI CACnn'nnGTG
Cuts at: 0 148 602 750
Size: 148 454 148
Fragments arranged by size:
454 148 148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 750
Size: 211 539
AlwNI CAG_nnn'CTG
Cuts at: 0 543 750
Size: 543 207
ApaLI G'TGCA_C
Cuts at: 0 268 750
Size: 268 482
AvaI C'yCGr_G
Cuts at: 0 653 750
Size: 653 97
BanII G_rGCy'C
Cuts at: 0 257 317 346 407 458 750
Size: 257 60 29 61 51 292
Fragments arranged by size:
292 257 61 60 51 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 57 501 750
Size: 57 444 249
Fragments arranged by size:
444 249 57
BlpI GC'TnA_GC
Cuts at: 0 286 750
Size: 286 464
Bme1580I G_kGCm'C
Cuts at: 0 272 750
Size: 272 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 49 79 601 655 729 750
Size: 43 6 30 522 54 74 21
Fragments arranged by size:
522 74 54 43 30 21 6
BsaWI w'CCGG_w
Cuts at: 0 275 479 750
Size: 275 204 271
Fragments arranged by size:
275 271 204
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 577 750
Size: 415 162 173
Fragments arranged by size:
415 173 162
BseYI C'CCAG_C
Cuts at: 0 339 515 750
Size: 339 176 235
Fragments arranged by size:
339 235 176
BsiHKAI G_wGCw'C
Cuts at: 0 272 317 407 458 750
Size: 272 45 90 51 292
Fragments arranged by size:
292 272 90 51 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 210 378 750
Size: 210 168 372
Fragments arranged by size:
372 210 168
Bsp1286I G_dGCh'C
Cuts at: 0 257 272 317 346 407 458 750
Size: 257 15 45 29 61 51 292
Fragments arranged by size:
292 257 61 51 45 29 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 162 750
Size: 162 588
BsrBI CCG'CTC
Cuts at: 0 285 660 750
Size: 285 375 90
Fragments arranged by size:
375 285 90
BsrDI GCAATG_nn'
Cuts at: 0 705 750
Size: 705 45
BsrFI r'CCGG_y
Cuts at: 0 35 750
Size: 35 715
BstAPI GCAn_nnn'nTGC
Cuts at: 0 113 750
Size: 113 637
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 750
Size: 57 693
Bsu36I CC'TnA_GG
Cuts at: 0 232 750
Size: 232 518
BtgI C'CryG_G
Cuts at: 0 603 737 750
Size: 603 134 13
Fragments arranged by size:
603 134 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 444 750
Size: 444 306
DraIII CAC_nnn'GTG
Cuts at: 0 555 750
Size: 555 195
EaeI y'GGCC_r
Cuts at: 0 93 750
Size: 93 657
EarI CTCTTCn'nnn_
Cuts at: 0 421 750
Size: 421 329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 514 750
Size: 514 236
EcoICRI GAG'CTC
Cuts at: 0 315 405 456 750
Size: 315 90 51 294
Fragments arranged by size:
315 294 90 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 43 49 79 448 495 601 655
Size: 25 18 6 30 369 47 106 54
Cuts at: 655 729 750
Size: 74 21
Fragments arranged by size:
369 106 74 54 47 30 25 21
18 6
EcoO109I rG'GnC_Cy
Cuts at: 0 246 750
Size: 246 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 579 611 750
Size: 579 32 139
Fragments arranged by size:
579 139 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 750
Size: 482 268
MslI CAynn'nnrTG
Cuts at: 0 148 602 750
Size: 148 454 148
Fragments arranged by size:
454 148 148
MspA1I CmG'CkG
Cuts at: 0 515 575 633 750
Size: 515 60 58 117
Fragments arranged by size:
515 117 60 58
NcoI C'CATG_G
Cuts at: 0 737 750
Size: 737 13
NspI r_CATG'y
Cuts at: 0 630 750
Size: 630 120
PasI CC'CwG_GG
Cuts at: 0 241 645 750
Size: 241 404 105
Fragments arranged by size:
404 241 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 167 555 738 750
Size: 167 388 183 12
Fragments arranged by size:
388 183 167 12
PfoI T'CCnGG_A
Cuts at: 0 536 750
Size: 536 214
PpuMI rG'GwC_Cy
Cuts at: 0 246 750
Size: 246 504
PstI C_TGCA'G
Cuts at: 0 388 750
Size: 388 362
PvuII CAG'CTG
Cuts at: 0 515 633 750
Size: 515 118 117
Fragments arranged by size:
515 118 117
SacI G_AGCT'C
Cuts at: 0 317 407 458 750
Size: 317 90 51 292
Fragments arranged by size:
317 292 90 51
SanDI GG'GwC_CC
Cuts at: 0 246 750
Size: 246 504
SfcI C'TryA_G
Cuts at: 0 85 384 750
Size: 85 299 366
Fragments arranged by size:
366 299 85
SmaI CCC'GGG
Cuts at: 0 655 750
Size: 655 95
StyI C'CwwG_G
Cuts at: 0 146 257 737 750
Size: 146 111 480 13
Fragments arranged by size:
480 146 111 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 207 507 750
Size: 207 300 243
Fragments arranged by size:
300 243 207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 750
Size: 207 543
XmaI C'CCGG_G
Cuts at: 0 653 750
Size: 653 97
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaLI AvaI BanII
BbsI BlpI Bme1580I BpmI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI
Bsu36I BtgI BtgZI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I Hin4I MmeI MslI MspA1I NcoI NspI
PasI PflMI PfoI PpuMI PstI PvuII SacI SanDI
SfcI SmaI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I BtsI ClaI CspCI DraI DrdI
EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI