(Linear) (Six-base) MAPSORT of: KIEE0260.seq Check: 1943 from: 1 to: 294 KIEE0260.orf With 182 enzymes: SgfI * April 24, 2009 13:12 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 201 294 Size: 201 93 BglII A'GATC_T Cuts at: 0 263 294 Size: 263 31 BsaWI w'CCGG_w Cuts at: 0 123 294 Size: 123 171 BspEI T'CCGG_A Cuts at: 0 123 294 Size: 123 171 BstYI r'GATC_y Cuts at: 0 263 294 Size: 263 31 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 201 294 Size: 201 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 248 280 294 Size: 248 32 14 Fragments arranged by size: 248 32 14 PfoI T'CCnGG_A Cuts at: 0 164 294 Size: 164 130 SfcI C'TryA_G Cuts at: 0 84 294 Size: 84 210 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 177 209 294 Size: 177 32 85 Fragments arranged by size: 177 85 32 Enzymes that do cut: AcuI BglII BsaWI BspEI BstYI Eco57MI Hin4I PfoI SfcI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI