Restriction map for
pF1KE0261
(Linear) (Six-base) MAPSORT of: KIEE0261.seq Check: 5605 from: 1 to: 1125
KIEE0261.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 347 1125
Size: 169 178 778
Fragments arranged by size:
778 178 169
AlwNI CAG_nnn'CTG
Cuts at: 0 673 1125
Size: 673 452
ApaI G_GGCC'C
Cuts at: 0 591 1125
Size: 591 534
BanI G'GyrC_C
Cuts at: 0 902 1125
Size: 902 223
BanII G_rGCy'C
Cuts at: 0 218 591 1125
Size: 218 373 534
Fragments arranged by size:
534 373 218
BbvCI CC'TCA_GC
Cuts at: 0 59 1125
Size: 59 1066
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 84 118 1125
Size: 84 34 1007
Fragments arranged by size:
1007 84 34
BglI GCCn_nnn'nGGC
Cuts at: 0 559 1125
Size: 559 566
BlpI GC'TnA_GC
Cuts at: 0 713 1125
Size: 713 412
Bme1580I G_kGCm'C
Cuts at: 0 591 1125
Size: 591 534
BmrI ACTGGGnnnn_n'
Cuts at: 0 263 1125
Size: 263 862
Bpu10I CC'TnA_GC
Cuts at: 0 59 1125
Size: 59 1066
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 1125
Size: 74 1051
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 774 1125
Size: 286 488 351
Fragments arranged by size:
488 351 286
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 667 928 994 1057 1060 1125
Size: 76 591 261 66 63 3 65
Fragments arranged by size:
591 261 76 66 65 63 3
BseYI C'CCAG_C
Cuts at: 0 19 1125
Size: 19 1106
BsiEI CG_ry'CG
Cuts at: 0 108 1125
Size: 108 1017
BsiHKAI G_wGCw'C
Cuts at: 0 218 1125
Size: 218 907
BsmBI CGTCTCn'nnnn_
Cuts at: 0 571 1125
Size: 571 554
Bsp1286I G_dGCh'C
Cuts at: 0 218 591 1125
Size: 218 373 534
Fragments arranged by size:
534 373 218
BsrBI CCG'CTC
Cuts at: 0 14 1125
Size: 14 1111
BsrDI GCAATG_nn'
Cuts at: 0 30 1125
Size: 30 1095
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1119 1125
Size: 1119 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 583 1125
Size: 583 542
Bsu36I CC'TnA_GG
Cuts at: 0 874 1125
Size: 874 251
BtgI C'CryG_G
Cuts at: 0 7 1125
Size: 7 1118
BtsI GCAGTG_nn'
Cuts at: 0 408 972 1125
Size: 408 564 153
Fragments arranged by size:
564 408 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 24 59 1125
Size: 24 35 1066
Fragments arranged by size:
1066 35 24
EaeI y'GGCC_r
Cuts at: 0 105 233 1125
Size: 105 128 892
Fragments arranged by size:
892 128 105
EagI C'GGCC_G
Cuts at: 0 105 1125
Size: 105 1020
EarI CTCTTCn'nnn_
Cuts at: 0 223 889 905 1021 1125
Size: 223 666 16 116 104
Fragments arranged by size:
666 223 116 104 16
EcoICRI GAG'CTC
Cuts at: 0 216 1125
Size: 216 909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 347 1125
Size: 169 178 778
Fragments arranged by size:
778 178 169
EcoNI CCTnn'n_nnAGG
Cuts at: 0 286 1125
Size: 286 839
EcoO109I rG'GnC_Cy
Cuts at: 0 579 587 588 1125
Size: 579 8 1 537
Fragments arranged by size:
579 537 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 339 1125
Size: 339 786
HindIII A'AGCT_T
Cuts at: 0 523 1125
Size: 523 602
MspA1I CmG'CkG
Cuts at: 0 9 69 1125
Size: 9 60 1056
Fragments arranged by size:
1056 60 9
NdeI CA'TA_TG
Cuts at: 0 999 1125
Size: 999 126
NspI r_CATG'y
Cuts at: 0 712 1122 1125
Size: 712 410 3
Fragments arranged by size:
712 410 3
PasI CC'CwG_GG
Cuts at: 0 583 1125
Size: 583 542
PpuMI rG'GwC_Cy
Cuts at: 0 579 1125
Size: 579 546
PsiI TTA'TAA
Cuts at: 0 305 1125
Size: 305 820
PspOMI G'GGCC_C
Cuts at: 0 587 1125
Size: 587 538
PstI C_TGCA'G
Cuts at: 0 403 602 822 963 1125
Size: 403 199 220 141 162
Fragments arranged by size:
403 220 199 162 141
PvuII CAG'CTG
Cuts at: 0 69 1125
Size: 69 1056
SacI G_AGCT'C
Cuts at: 0 218 1125
Size: 218 907
SacII CC_GC'GG
Cuts at: 0 10 1125
Size: 10 1115
SapI GCTCTTCn'nnn_
Cuts at: 0 223 1125
Size: 223 902
SfcI C'TryA_G
Cuts at: 0 399 598 665 818 959 1125
Size: 399 199 67 153 141 166
Fragments arranged by size:
399 199 166 153 141 67
SmlI C'TyrA_G
Cuts at: 0 89 1125
Size: 89 1036
SphI G_CATG'C
Cuts at: 0 1122 1125
Size: 1122 3
StuI AGG'CCT
Cuts at: 0 101 553 1125
Size: 101 452 572
Fragments arranged by size:
572 452 101
StyI C'CwwG_G
Cuts at: 0 185 1125
Size: 185 940
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 716 1125
Size: 716 409
TatI w'GTAC_w
Cuts at: 0 267 1125
Size: 267 858
Tth111I GACn'n_nGTC
Cuts at: 0 31 1125
Size: 31 1094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 1125
Size: 243 882
Enzymes that do cut:
AcuI AlwNI ApaI BanI BanII BbvCI BcgI BglI
BlpI Bme1580I BmrI Bpu10I BpuEI BsaI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BstAPI BstXI
Bsu36I BtgI BtsI CspCI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HindIII MspA1I NdeI NspI
PasI PpuMI PsiI PspOMI PstI PvuII SacI SacII
SapI SfcI SmlI SphI StuI StyI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BciVI BclI BfrBI BglII BmgBI BmtI BplI BpmI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BsmI
BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI
EciI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PspXI PsrI PvuI RsrII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TstI XbaI XhoI XmaI
XmnI ZraI