(Linear) (Six-base) MAPSORT of: KIEE0262.seq  Check: 9393  from: 1  to: 453



KIEE0262.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     453 
   Size:        196     257
 
ApaI G_GGCC'C

Cuts at:      0     110     453 
   Size:        110     343
 
BanII G_rGCy'C

Cuts at:      0     110     349     453 
   Size:        110     239     104

  Fragments arranged by size: 

                 239     110     104
 
BglI GCCn_nnn'nGGC

Cuts at:      0     166     453 
   Size:        166     287
 
Bme1580I G_kGCm'C

Cuts at:      0     110     453 
   Size:        110     343
 
BmgBI CAC'GTC

Cuts at:      0      45     453 
   Size:         45     408
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     144     453 
   Size:        144     309
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     453 
   Size:        425      28
 
BsaWI w'CCGG_w

Cuts at:      0      32     453 
   Size:         32     421
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     422     453 
   Size:        422      31
 
BsiHKAI G_wGCw'C

Cuts at:      0     248     453 
   Size:        248     205
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     248     349     453 
   Size:        110     138     101     104

  Fragments arranged by size: 

                 138     110     104     101
 
BspEI T'CCGG_A

Cuts at:      0      32     453 
   Size:         32     421
 
BsrDI GCAATG_nn'

Cuts at:      0     385     453 
   Size:        385      68
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     400     453 
   Size:        400      53
 
BstYI r'GATC_y

Cuts at:      0     309     453 
   Size:        309     144
 
BtsI GCAGTG_nn'

Cuts at:      0     215     369     453 
   Size:        215     154      84

  Fragments arranged by size: 

                 215     154      84
 
DraI TTT'AAA

Cuts at:      0     258     453 
   Size:        258     195
 
EaeI y'GGCC_r

Cuts at:      0      96     453 
   Size:         96     357
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     425     453 
   Size:        196     229      28

  Fragments arranged by size: 

                 229     196      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     334     366     453 
   Size:        334      32      87

  Fragments arranged by size: 

                 334      87      32
 
MspA1I CmG'CkG

Cuts at:      0     157     453 
   Size:        157     296
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84     453 
   Size:         52      32     369

  Fragments arranged by size: 

                 369      52      32
 
PsiI TTA'TAA

Cuts at:      0     332     453 
   Size:        332     121
 
PspOMI G'GGCC_C

Cuts at:      0     106     453 
   Size:        106     347
 
PvuII CAG'CTG

Cuts at:      0     157     453 
   Size:        157     296
 
SfcI C'TryA_G

Cuts at:      0     163     453 
   Size:        163     290
 
XmnI GAAnn'nnTTC

Cuts at:      0      67     453 
   Size:         67     386
 

 Enzymes that do cut:

     AcuI     ApaI    BanII     BglI Bme1580I    BmgBI     BplI     BpmI
    BsaWI    BseRI  BsiHKAI Bsp1286I    BspEI    BsrDI   BstAPI    BstYI
     BtsI     DraI     EaeI  Eco57MI    Hin4I   MspA1I     PpiI     PsiI
   PspOMI    PvuII     SfcI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI