Restriction map for pF1KE0263
 (Linear) (Six-base) MAPSORT of: KIEE0263.seq  Check: 9075  from: 1  to: 396



KIEE0263.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

ApoI r'AATT_y

Cuts at:      0      78     208     396 
   Size:         78     130     188

  Fragments arranged by size: 

                 188     130      78
 
BanII G_rGCy'C

Cuts at:      0      11     396 
   Size:         11     385
 
BglII A'GATC_T

Cuts at:      0     185     396 
   Size:        185     211
 
BmgBI CAC'GTC

Cuts at:      0     282     396 
   Size:        282     114
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      79     396 
   Size:         79     317
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     285     396 
   Size:        285     111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     396 
   Size:         21     375
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      39     396 
   Size:         39     357
 
BsaWI w'CCGG_w

Cuts at:      0     233     396 
   Size:        233     163
 
BseYI C'CCAG_C

Cuts at:      0     198     396 
   Size:        198     198
 
BsiHKAI G_wGCw'C

Cuts at:      0     250     396 
   Size:        250     146
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     250     396 
   Size:         11     239     146

  Fragments arranged by size: 

                 239     146      11
 
BstYI r'GATC_y

Cuts at:      0     185     396 
   Size:        185     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     396 
   Size:         21     375
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     207     396 
   Size:        207     189
 
PfoI T'CCnGG_A

Cuts at:      0     173     396 
   Size:        173     223
 
SfcI C'TryA_G

Cuts at:      0     221     396 
   Size:        221     175
 
SspI AAT'ATT

Cuts at:      0     366     396 
   Size:        366      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     292     396 
   Size:        292     104
 

 Enzymes that do cut:

     ApoI    BanII    BglII    BmgBI     BmrI     BplI     BpmI     BsaI
    BsaWI    BseYI  BsiHKAI Bsp1286I    BstYI  Eco57MI     FalI     PfoI
     SfcI     SspI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com