(Linear) (Six-base) MAPSORT of: KIEE0263.seq Check: 9075 from: 1 to: 396 KIEE0263.orf With 182 enzymes: SgfI * April 24, 2009 13:12 .. ApoI r'AATT_y Cuts at: 0 78 208 396 Size: 78 130 188 Fragments arranged by size: 188 130 78 BanII G_rGCy'C Cuts at: 0 11 396 Size: 11 385 BglII A'GATC_T Cuts at: 0 185 396 Size: 185 211 BmgBI CAC'GTC Cuts at: 0 282 396 Size: 282 114 BmrI ACTGGGnnnn_n' Cuts at: 0 79 396 Size: 79 317 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 285 396 Size: 285 111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 396 Size: 21 375 BsaI GGTCTCn'nnnn_ Cuts at: 0 39 396 Size: 39 357 BsaWI w'CCGG_w Cuts at: 0 233 396 Size: 233 163 BseYI C'CCAG_C Cuts at: 0 198 396 Size: 198 198 BsiHKAI G_wGCw'C Cuts at: 0 250 396 Size: 250 146 Bsp1286I G_dGCh'C Cuts at: 0 11 250 396 Size: 11 239 146 Fragments arranged by size: 239 146 11 BstYI r'GATC_y Cuts at: 0 185 396 Size: 185 211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 396 Size: 21 375 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 207 396 Size: 207 189 PfoI T'CCnGG_A Cuts at: 0 173 396 Size: 173 223 SfcI C'TryA_G Cuts at: 0 221 396 Size: 221 175 SspI AAT'ATT Cuts at: 0 366 396 Size: 366 30 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 292 396 Size: 292 104 Enzymes that do cut: ApoI BanII BglII BmgBI BmrI BplI BpmI BsaI BsaWI BseYI BsiHKAI Bsp1286I BstYI Eco57MI FalI PfoI SfcI SspI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI