Restriction map for
pF1KE0269
(Linear) (Six-base) MAPSORT of: KIEE0269.seq Check: 8508 from: 1 to: 1299
KIEE0269.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1055 1299
Size: 1055 244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 946 1299
Size: 517 429 353
Fragments arranged by size:
517 429 353
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 1299
Size: 122 32 1145
Fragments arranged by size:
1145 122 32
AlwNI CAG_nnn'CTG
Cuts at: 0 133 514 538 1017 1216 1299
Size: 133 381 24 479 199 83
Fragments arranged by size:
479 381 199 133 83 24
ApaI G_GGCC'C
Cuts at: 0 1180 1299
Size: 1180 119
ApoI r'AATT_y
Cuts at: 0 21 780 1299
Size: 21 759 519
Fragments arranged by size:
759 519 21
AvaI C'yCGr_G
Cuts at: 0 1082 1281 1299
Size: 1082 199 18
Fragments arranged by size:
1082 199 18
AvrII C'CTAG_G
Cuts at: 0 1168 1299
Size: 1168 131
BamHI G'GATC_C
Cuts at: 0 1102 1299
Size: 1102 197
BanI G'GyrC_C
Cuts at: 0 868 1043 1299
Size: 868 175 256
Fragments arranged by size:
868 256 175
BanII G_rGCy'C
Cuts at: 0 1180 1299
Size: 1180 119
BbsI GAAGACnn'nnnn_
Cuts at: 0 1293 1299
Size: 1293 6
Bme1580I G_kGCm'C
Cuts at: 0 847 1180 1299
Size: 847 333 119
Fragments arranged by size:
847 333 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 524 622 1299
Size: 524 98 677
Fragments arranged by size:
677 524 98
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 159 514 1299
Size: 159 355 785
Fragments arranged by size:
785 355 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1027 1299
Size: 970 57 272
Fragments arranged by size:
970 272 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 1133 1299
Size: 1133 166
BsaBI GATnn'nnATC
Cuts at: 0 278 1299
Size: 278 1021
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 95 125 1299
Size: 95 30 1174
Fragments arranged by size:
1174 95 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 1299
Size: 304 995
BseYI C'CCAG_C
Cuts at: 0 889 1031 1173 1299
Size: 889 142 142 126
Fragments arranged by size:
889 142 142 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1299
Size: 847 452
BsiHKAI G_wGCw'C
Cuts at: 0 993 1163 1299
Size: 993 170 136
Fragments arranged by size:
993 170 136
Bsp1286I G_dGCh'C
Cuts at: 0 847 993 1163 1180 1299
Size: 847 146 170 17 119
Fragments arranged by size:
847 170 146 119 17
BspHI T'CATG_A
Cuts at: 0 449 1299
Size: 449 850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1055 1299
Size: 1055 244
BsrFI r'CCGG_y
Cuts at: 0 1022 1040 1299
Size: 1022 18 259
Fragments arranged by size:
1022 259 18
BssSI C'ACGA_G
Cuts at: 0 148 391 1299
Size: 148 243 908
Fragments arranged by size:
908 243 148
BstAPI GCAn_nnn'nTGC
Cuts at: 0 538 1299
Size: 538 761
BstBI TT'CG_AA
Cuts at: 0 87 1299
Size: 87 1212
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 612 1299
Size: 417 195 687
Fragments arranged by size:
687 417 195
BstYI r'GATC_y
Cuts at: 0 723 1102 1299
Size: 723 379 197
Fragments arranged by size:
723 379 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 264 1299
Size: 264 1035
EaeI y'GGCC_r
Cuts at: 0 419 743 788 854 1233 1299
Size: 419 324 45 66 379 66
Fragments arranged by size:
419 379 324 66 66 45
EarI CTCTTCn'nnn_
Cuts at: 0 233 432 1299
Size: 233 199 867
Fragments arranged by size:
867 233 199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1027 1299
Size: 970 57 272
Fragments arranged by size:
970 272 57
EcoO109I rG'GnC_Cy
Cuts at: 0 616 1177 1278 1299
Size: 616 561 101 21
Fragments arranged by size:
616 561 101 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 1299
Size: 95 32 1172
Fragments arranged by size:
1172 95 32
HincII GTy'rAC
Cuts at: 0 191 381 1299
Size: 191 190 918
Fragments arranged by size:
918 191 190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 776 1299
Size: 148 628 523
Fragments arranged by size:
628 523 148
MscI TGG'CCA
Cuts at: 0 421 745 790 856 1235 1299
Size: 421 324 45 66 379 64
Fragments arranged by size:
421 379 324 66 64 45
MslI CAynn'nnrTG
Cuts at: 0 203 769 1299
Size: 203 566 530
Fragments arranged by size:
566 530 203
MspA1I CmG'CkG
Cuts at: 0 538 1299
Size: 538 761
PspOMI G'GGCC_C
Cuts at: 0 1176 1299
Size: 1176 123
PstI C_TGCA'G
Cuts at: 0 935 1299
Size: 935 364
PvuII CAG'CTG
Cuts at: 0 538 1299
Size: 538 761
SapI GCTCTTCn'nnn_
Cuts at: 0 233 432 1299
Size: 233 199 867
Fragments arranged by size:
867 233 199
SfcI C'TryA_G
Cuts at: 0 931 1299
Size: 931 368
SmaI CCC'GGG
Cuts at: 0 1084 1283 1299
Size: 1084 199 16
Fragments arranged by size:
1084 199 16
StyI C'CwwG_G
Cuts at: 0 41 1168 1222 1299
Size: 41 1127 54 77
Fragments arranged by size:
1127 77 54 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 864 871 1045 1299
Size: 864 7 174 254
Fragments arranged by size:
864 254 174 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1094 1126 1299
Size: 1094 32 173
Fragments arranged by size:
1094 173 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 1299
Size: 411 888
XmaI C'CCGG_G
Cuts at: 0 1082 1281 1299
Size: 1082 199 18
Fragments arranged by size:
1082 199 18
XmnI GAAnn'nnTTC
Cuts at: 0 582 1299
Size: 582 717
Enzymes that do cut:
AarI AlfI AloI AlwNI ApaI ApoI AvaI AvrII
BamHI BanI BanII BbsI Bme1580I BmrI BplI BpmI
BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI EaeI EarI Eco57MI EcoO109I Hin4I HincII MmeI
MscI MslI MspA1I PspOMI PstI PvuII SapI SfcI
SmaI StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI ApaLI AscI AseI AsiSI BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI ZraI