(Linear) (Six-base) MAPSORT of: KIEE0279.seq  Check: 7320  from: 1  to: 624



KIEE0279.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AccI GT'mk_AC

Cuts at:      0     272     491     624 
   Size:        272     219     133

  Fragments arranged by size: 

                 272     219     133
 
AhdI GACnn_n'nnGTC

Cuts at:      0     348     624 
   Size:        348     276
 
ApoI r'AATT_y

Cuts at:      0     217     624 
   Size:        217     407
 
BanI G'GyrC_C

Cuts at:      0     595     624 
   Size:        595      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146     624 
   Size:        146     478
 
BglII A'GATC_T

Cuts at:      0     107     624 
   Size:        107     517
 
Bme1580I G_kGCm'C

Cuts at:      0      84     624 
   Size:         84     540
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      14     624 
   Size:         14     610
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     417     624 
   Size:        417     207
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     575     624 
   Size:        575      49
 
BsaAI yAC'GTr

Cuts at:      0     306     624 
   Size:        306     318
 
BsaWI w'CCGG_w

Cuts at:      0     537     624 
   Size:        537      87
 
BseYI C'CCAG_C

Cuts at:      0     205     624 
   Size:        205     419
 
BsiHKAI G_wGCw'C

Cuts at:      0     305     624 
   Size:        305     319
 
Bsp1286I G_dGCh'C

Cuts at:      0      84     305     624 
   Size:         84     221     319

  Fragments arranged by size: 

                 319     221      84
 
BspEI T'CCGG_A

Cuts at:      0     537     624 
   Size:        537      87
 
BsrDI GCAATG_nn'

Cuts at:      0     616     624 
   Size:        616       8
 
BssSI C'ACGA_G

Cuts at:      0     367     624 
   Size:        367     257
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     243     624 
   Size:        243     381
 
BstYI r'GATC_y

Cuts at:      0     107     624 
   Size:        107     517
 
EaeI y'GGCC_r

Cuts at:      0     371     624 
   Size:        371     253
 
EcoO109I rG'GnC_Cy

Cuts at:      0     474     624 
   Size:        474     150
 
EcoRI G'AATT_C

Cuts at:      0     217     624 
   Size:        217     407
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     312     624 
   Size:        312     312
 
FspI TGC'GCA

Cuts at:      0     247     624 
   Size:        247     377
 
FspAI rTGC'GCAy

Cuts at:      0     247     624 
   Size:        247     377
 
HaeII r_GCGC'y

Cuts at:      0      14     227     624 
   Size:         14     213     397

  Fragments arranged by size: 

                 397     213      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     156     188     334     366     624 
   Size:        156      32     146      32     258

  Fragments arranged by size: 

                 258     156     146      32      32
 
HincII GTy'rAC

Cuts at:      0     492     624 
   Size:        492     132
 
MscI TGG'CCA

Cuts at:      0     373     624 
   Size:        373     251
 
MspA1I CmG'CkG

Cuts at:      0     205     624 
   Size:        205     419
 
NspI r_CATG'y

Cuts at:      0     246     624 
   Size:        246     378
 
PmlI CAC'GTG

Cuts at:      0     306     624 
   Size:        306     318
 
PpuMI rG'GwC_Cy

Cuts at:      0     474     624 
   Size:        474     150
 
PvuII CAG'CTG

Cuts at:      0     205     624 
   Size:        205     419
 
SalI G'TCGA_C

Cuts at:      0     490     624 
   Size:        490     134
 
SmlI C'TyrA_G

Cuts at:      0     396     624 
   Size:        396     228
 
SspI AAT'ATT

Cuts at:      0     447     624 
   Size:        447     177
 
StyI C'CwwG_G

Cuts at:      0     470     624 
   Size:        470     154
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     613     624 
   Size:        613      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     624 
   Size:         42     582
 

 Enzymes that do cut:

     AccI     AhdI     ApoI     BanI     BbsI    BglII Bme1580I     BmrI
    BpuEI     BsaI    BsaAI    BsaWI    BseYI  BsiHKAI Bsp1286I    BspEI
    BsrDI    BssSI   BstAPI    BstYI     EaeI EcoO109I    EcoRI     FalI
     FspI    FspAI    HaeII    Hin4I   HincII     MscI   MspA1I     NspI
     PmlI    PpuMI    PvuII     SalI     SmlI     SspI     StyI    TaqII
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BsaBI    BsaHI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRV     FseI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI