Restriction map for
pF1KE0281
(Linear) (Six-base) MAPSORT of: KIEE0281.seq Check: 5044 from: 1 to: 702
KIEE0281.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 154 370 547 702
Size: 154 216 177 155
Fragments arranged by size:
216 177 155 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 702
Size: 279 423
AleI CACnn'nnGTG
Cuts at: 0 399 702
Size: 399 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 555 702
Size: 223 332 147
Fragments arranged by size:
332 223 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 124 156 702
Size: 124 32 546
Fragments arranged by size:
546 124 32
BanI G'GyrC_C
Cuts at: 0 6 632 702
Size: 6 626 70
Fragments arranged by size:
626 70 6
BanII G_rGCy'C
Cuts at: 0 445 604 702
Size: 445 159 98
Fragments arranged by size:
445 159 98
BbeI G_GCGC'C
Cuts at: 0 10 636 702
Size: 10 626 66
Fragments arranged by size:
626 66 10
BglI GCCn_nnn'nGGC
Cuts at: 0 95 182 197 371 689 702
Size: 95 87 15 174 318 13
Fragments arranged by size:
318 174 95 87 15 13
Bme1580I G_kGCm'C
Cuts at: 0 85 376 702
Size: 85 291 326
Fragments arranged by size:
326 291 85
BmgBI CAC'GTC
Cuts at: 0 672 702
Size: 672 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 430 702
Size: 430 272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 508 702
Size: 459 49 194
Fragments arranged by size:
459 194 49
BsaHI Gr'CG_yC
Cuts at: 0 7 633 702
Size: 7 626 69
Fragments arranged by size:
626 69 7
BsaWI w'CCGG_w
Cuts at: 0 314 702
Size: 314 388
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 541 612 702
Size: 142 399 71 90
Fragments arranged by size:
399 142 90 71
BseYI C'CCAG_C
Cuts at: 0 517 702
Size: 517 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 193 432 702
Size: 87 106 239 270
Fragments arranged by size:
270 239 106 87
Bsp1286I G_dGCh'C
Cuts at: 0 85 376 445 604 702
Size: 85 291 69 159 98
Fragments arranged by size:
291 159 98 85 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 154 370 475 547 702
Size: 154 216 105 72 155
Fragments arranged by size:
216 155 154 105 72
BsrFI r'CCGG_y
Cuts at: 0 629 702
Size: 629 73
BsrGI T'GTAC_A
Cuts at: 0 338 702
Size: 338 364
BstAPI GCAn_nnn'nTGC
Cuts at: 0 317 702
Size: 317 385
BtgI C'CryG_G
Cuts at: 0 41 240 702
Size: 41 199 462
Fragments arranged by size:
462 199 41
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 252 287 702
Size: 252 35 415
Fragments arranged by size:
415 252 35
DraIII CAC_nnn'GTG
Cuts at: 0 42 702
Size: 42 660
EaeI y'GGCC_r
Cuts at: 0 243 510 606 702
Size: 243 267 96 96
Fragments arranged by size:
267 243 96 96
EarI CTCTTCn'nnn_
Cuts at: 0 166 702
Size: 166 536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 178 702
Size: 163 15 524
Fragments arranged by size:
524 163 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 459 508 702
Size: 279 180 49 194
Fragments arranged by size:
279 194 180 49
EcoO109I rG'GnC_Cy
Cuts at: 0 424 483 581 702
Size: 424 59 98 121
Fragments arranged by size:
424 121 98 59
HaeII r_GCGC'y
Cuts at: 0 10 636 702
Size: 10 626 66
Fragments arranged by size:
626 66 10
HincII GTy'rAC
Cuts at: 0 255 702
Size: 255 447
KasI G'GCGC_C
Cuts at: 0 6 632 702
Size: 6 626 70
Fragments arranged by size:
626 70 6
MslI CAynn'nnrTG
Cuts at: 0 399 702
Size: 399 303
MspA1I CmG'CkG
Cuts at: 0 242 702
Size: 242 460
NarI GG'CG_CC
Cuts at: 0 7 633 702
Size: 7 626 69
Fragments arranged by size:
626 69 7
NspI r_CATG'y
Cuts at: 0 51 702
Size: 51 651
PasI CC'CwG_GG
Cuts at: 0 223 428 702
Size: 223 205 274
Fragments arranged by size:
274 223 205
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 702
Size: 42 660
PpuMI rG'GwC_Cy
Cuts at: 0 424 702
Size: 424 278
PstI C_TGCA'G
Cuts at: 0 698 702
Size: 698 4
SacII CC_GC'GG
Cuts at: 0 243 702
Size: 243 459
SanDI GG'GwC_CC
Cuts at: 0 424 702
Size: 424 278
SapI GCTCTTCn'nnn_
Cuts at: 0 166 702
Size: 166 536
SfcI C'TryA_G
Cuts at: 0 694 702
Size: 694 8
SfoI GGC'GCC
Cuts at: 0 8 634 702
Size: 8 626 68
Fragments arranged by size:
626 68 8
TatI w'GTAC_w
Cuts at: 0 338 702
Size: 338 364
Enzymes that do cut:
AarI AcuI AleI AlfI AloI BanI BanII BbeI
BglI Bme1580I BmgBI BmrI BpmI BsaHI BsaWI BseRI
BseYI BsgI Bsp1286I BspMI BsrFI BsrGI BstAPI BtgI
CspCI DraIII EaeI EarI EciI Eco57MI EcoO109I HaeII
HincII KasI MslI MspA1I NarI NspI PasI PflMI
PpuMI PstI SacII SanDI SapI SfcI SfoI TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI