Restriction map for
pF1KE0282
(Linear) (Six-base) MAPSORT of: KIEE0282.seq Check: 1788 from: 1 to: 1296
KIEE0282.orf
With 182 enzymes: SgfI *
October 18, 2013 09:44 ..
AccI GT'mk_AC
Cuts at: 0 529 891 1296
Size: 529 362 405
Fragments arranged by size:
529 405 362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1295 1296
Size: 464 831 1
Fragments arranged by size:
831 464 1
AflIII A'CryG_T
Cuts at: 0 151 1296
Size: 151 1145
AhdI GACnn_n'nnGTC
Cuts at: 0 1287 1296
Size: 1287 9
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 1296
Size: 94 1202
ApoI r'AATT_y
Cuts at: 0 179 383 1296
Size: 179 204 913
Fragments arranged by size:
913 204 179
AvaI C'yCGr_G
Cuts at: 0 617 932 1296
Size: 617 315 364
Fragments arranged by size:
617 364 315
BanII G_rGCy'C
Cuts at: 0 220 852 1296
Size: 220 632 444
Fragments arranged by size:
632 444 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 335 1208 1268 1296
Size: 335 873 60 28
Fragments arranged by size:
873 335 60 28
BglI GCCn_nnn'nGGC
Cuts at: 0 365 1296
Size: 365 931
BglII A'GATC_T
Cuts at: 0 1030 1296
Size: 1030 266
BmrI ACTGGGnnnn_n'
Cuts at: 0 606 1296
Size: 606 690
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 234 1296
Size: 234 1062
Bpu10I CC'TnA_GC
Cuts at: 0 514 1296
Size: 514 782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 882 1296
Size: 882 414
BsaI GGTCTCn'nnnn_
Cuts at: 0 935 1296
Size: 935 361
BsaBI GATnn'nnATC
Cuts at: 0 191 1296
Size: 191 1105
BsaWI w'CCGG_w
Cuts at: 0 1177 1296
Size: 1177 119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 963 993 1083 1113 1296
Size: 963 30 90 30 183
Fragments arranged by size:
963 183 90 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1296
Size: 533 763
BsiEI CG_ry'CG
Cuts at: 0 11 1296
Size: 11 1285
BsiHKAI G_wGCw'C
Cuts at: 0 220 1296
Size: 220 1076
Bsp1286I G_dGCh'C
Cuts at: 0 220 852 1296
Size: 220 632 444
Fragments arranged by size:
632 444 220
BspHI T'CATG_A
Cuts at: 0 183 227 1296
Size: 183 44 1069
Fragments arranged by size:
1069 183 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 917 1296
Size: 917 379
BssSI C'ACGA_G
Cuts at: 0 91 851 1296
Size: 91 760 445
Fragments arranged by size:
760 445 91
BstYI r'GATC_y
Cuts at: 0 1030 1147 1296
Size: 1030 117 149
Fragments arranged by size:
1030 149 117
ClaI AT'CG_AT
Cuts at: 0 1011 1296
Size: 1011 285
EaeI y'GGCC_r
Cuts at: 0 8 1296
Size: 8 1288
EagI C'GGCC_G
Cuts at: 0 8 1296
Size: 8 1288
EarI CTCTTCn'nnn_
Cuts at: 0 208 494 818 1296
Size: 208 286 324 478
Fragments arranged by size:
478 324 286 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 984 1296
Size: 984 312
EcoICRI GAG'CTC
Cuts at: 0 218 1296
Size: 218 1078
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1295 1296
Size: 464 831 1
Fragments arranged by size:
831 464 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 1060 1083 1092 1108 1115 1140
Size: 598 32 430 23 9 16 7 25
Cuts at: 1140 1255 1273 1287 1296
Size: 115 18 14 9
Fragments arranged by size:
598 430 115 32 25 23 18 16
14 9 9 7
HincII GTy'rAC
Cuts at: 0 530 1296
Size: 530 766
HindIII A'AGCT_T
Cuts at: 0 540 1296
Size: 540 756
MfeI C'AATT_G
Cuts at: 0 533 1296
Size: 533 763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 1296
Size: 2 1294
MspA1I CmG'CkG
Cuts at: 0 355 1296
Size: 355 941
PasI CC'CwG_GG
Cuts at: 0 465 1296
Size: 465 831
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 1296
Size: 636 660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 864 896 1296
Size: 864 32 400
Fragments arranged by size:
864 400 32
SacI G_AGCT'C
Cuts at: 0 220 1296
Size: 220 1076
SalI G'TCGA_C
Cuts at: 0 528 1296
Size: 528 768
SapI GCTCTTCn'nnn_
Cuts at: 0 208 1296
Size: 208 1088
SfcI C'TryA_G
Cuts at: 0 677 1296
Size: 677 619
SmaI CCC'GGG
Cuts at: 0 619 1296
Size: 619 677
SmlI C'TyrA_G
Cuts at: 0 897 1296
Size: 897 399
SspI AAT'ATT
Cuts at: 0 109 1296
Size: 109 1187
StyI C'CwwG_G
Cuts at: 0 1127 1296
Size: 1127 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 59 1296
Size: 59 1237
TatI w'GTAC_w
Cuts at: 0 411 1296
Size: 411 885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 343 1296
Size: 343 953
XmaI C'CCGG_G
Cuts at: 0 617 1296
Size: 617 679
XmnI GAAnn'nnTTC
Cuts at: 0 179 383 879 1296
Size: 179 204 496 417
Fragments arranged by size:
496 417 204 179
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI ApoI AvaI BanII
BbsI BglI BglII BmrI BplI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BssSI BstYI ClaI EaeI EagI EarI EciI
EcoICRI Eco57MI Hin4I HincII HindIII MfeI MmeI MspA1I
PasI PflMI PpiI SacI SalI SapI SfcI SmaI
SmlI SspI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BlpI Bme1580I BmgBI BmtI BpmI BsaAI BsaHI
BseRI BseYI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XhoI ZraI