Restriction map for
pF1KE0283
(Linear) (Six-base) MAPSORT of: KIEE0283.seq Check: 2540 from: 1 to: 969
KIEE0283.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
Acc65I G'GTAC_C
Cuts at: 0 392 969
Size: 392 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 969
Size: 574 395
AflIII A'CryG_T
Cuts at: 0 572 718 969
Size: 572 146 251
Fragments arranged by size:
572 251 146
AhdI GACnn_n'nnGTC
Cuts at: 0 258 969
Size: 258 711
AleI CACnn'nnGTG
Cuts at: 0 775 969
Size: 775 194
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 909 941 969
Size: 909 32 28
Fragments arranged by size:
909 32 28
ApoI r'AATT_y
Cuts at: 0 331 969
Size: 331 638
AvaI C'yCGr_G
Cuts at: 0 73 512 969
Size: 73 439 457
Fragments arranged by size:
457 439 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 210 243 969
Size: 210 33 726
Fragments arranged by size:
726 210 33
BanI G'GyrC_C
Cuts at: 0 392 969
Size: 392 577
BbsI GAAGACnn'nnnn_
Cuts at: 0 7 87 969
Size: 7 80 882
Fragments arranged by size:
882 80 7
BbvCI CC'TCA_GC
Cuts at: 0 692 969
Size: 692 277
BciVI GTATCCnnnnn_n'
Cuts at: 0 204 969
Size: 204 765
BclI T'GATC_A
Cuts at: 0 956 969
Size: 956 13
BlpI GC'TnA_GC
Cuts at: 0 190 969
Size: 190 779
BmgBI CAC'GTC
Cuts at: 0 575 969
Size: 575 394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 232 785 969
Size: 4 228 553 184
Fragments arranged by size:
553 228 184 4
Bpu10I CC'TnA_GC
Cuts at: 0 692 969
Size: 692 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 969
Size: 894 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 580 969
Size: 352 228 389
Fragments arranged by size:
389 352 228
BsaAI yAC'GTr
Cuts at: 0 719 969
Size: 719 250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 909 939 969
Size: 142 30 737 30 30
Fragments arranged by size:
737 142 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 969
Size: 391 578
BseYI C'CCAG_C
Cuts at: 0 342 543 969
Size: 342 201 426
Fragments arranged by size:
426 342 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 969
Size: 314 655
BspHI T'CATG_A
Cuts at: 0 134 404 969
Size: 134 270 565
Fragments arranged by size:
565 270 134
BsrFI r'CCGG_y
Cuts at: 0 698 969
Size: 698 271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 419 969
Size: 419 550
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 969
Size: 108 861
BstYI r'GATC_y
Cuts at: 0 508 539 969
Size: 508 31 430
Fragments arranged by size:
508 430 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 232 574 785 969
Size: 4 228 342 211 184
Fragments arranged by size:
342 228 211 184 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 969
Size: 262 707
EcoO109I rG'GnC_Cy
Cuts at: 0 252 969
Size: 252 717
EcoRI G'AATT_C
Cuts at: 0 331 969
Size: 331 638
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 969
Size: 244 32 693
Fragments arranged by size:
693 244 32
KpnI G_GTAC'C
Cuts at: 0 396 969
Size: 396 573
MslI CAynn'nnrTG
Cuts at: 0 106 151 175 643 736 775 847 943
Size: 106 45 24 468 93 39 72 96
Cuts at: 943 969
Size: 26
Fragments arranged by size:
468 106 96 93 72 45 39 26
24
NaeI GCC'GGC
Cuts at: 0 700 969
Size: 700 269
NgoMIV G'CCGG_C
Cuts at: 0 698 969
Size: 698 271
PflMI CCAn_nnn'nTGG
Cuts at: 0 735 969
Size: 735 234
PfoI T'CCnGG_A
Cuts at: 0 248 969
Size: 248 721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 909 941 969
Size: 909 32 28
Fragments arranged by size:
909 32 28
PpuMI rG'GwC_Cy
Cuts at: 0 252 969
Size: 252 717
PshAI GACnn'nnGTC
Cuts at: 0 481 969
Size: 481 488
PstI C_TGCA'G
Cuts at: 0 897 969
Size: 897 72
ScaI AGT'ACT
Cuts at: 0 911 969
Size: 911 58
SfcI C'TryA_G
Cuts at: 0 504 867 893 969
Size: 504 363 26 76
Fragments arranged by size:
504 363 76 26
SmaI CCC'GGG
Cuts at: 0 75 969
Size: 75 894
SmlI C'TyrA_G
Cuts at: 0 873 969
Size: 873 96
StuI AGG'CCT
Cuts at: 0 50 969
Size: 50 919
StyI C'CwwG_G
Cuts at: 0 263 458 969
Size: 263 195 511
Fragments arranged by size:
511 263 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 189 969
Size: 73 116 780
Fragments arranged by size:
780 116 73
TatI w'GTAC_w
Cuts at: 0 179 909 969
Size: 179 730 60
Fragments arranged by size:
730 179 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 142 174 969
Size: 142 32 795
Fragments arranged by size:
795 142 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 282 969
Size: 108 174 687
Fragments arranged by size:
687 174 108
XmaI C'CCGG_G
Cuts at: 0 73 969
Size: 73 896
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AloI ApoI AvaI
BaeI BanI BbsI BbvCI BciVI BclI BlpI BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI
BsgI BspHI BsrFI BstAPI BstXI BstYI Eco57MI EcoNI
EcoO109I EcoRI Hin4I KpnI MslI NaeI NgoMIV PflMI
PfoI PpiI PpuMI PshAI PstI ScaI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BamHI
BanII BbeI BcgI BfrBI BglI BglII Bme1580I BmrI
BmtI BplI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MmeI
MscI MspA1I NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI