Restriction map for
pF1KE0288
(Linear) (Six-base) MAPSORT of: KIEE0288.seq Check: 7218 from: 1 to: 939
KIEE0288.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 0 605 939
Size: 605 334
Acc65I G'GTAC_C
Cuts at: 0 368 939
Size: 368 571
AlwNI CAG_nnn'CTG
Cuts at: 0 390 939
Size: 390 549
ApaI G_GGCC'C
Cuts at: 0 515 939
Size: 515 424
ApoI r'AATT_y
Cuts at: 0 47 939
Size: 47 892
BamHI G'GATC_C
Cuts at: 0 457 939
Size: 457 482
BanI G'GyrC_C
Cuts at: 0 368 704 939
Size: 368 336 235
Fragments arranged by size:
368 336 235
BanII G_rGCy'C
Cuts at: 0 474 515 732 939
Size: 474 41 217 207
Fragments arranged by size:
474 217 207 41
BbeI G_GCGC'C
Cuts at: 0 708 939
Size: 708 231
BciVI GTATCCnnnnn_n'
Cuts at: 0 65 939
Size: 65 874
BglI GCCn_nnn'nGGC
Cuts at: 0 451 939
Size: 451 488
BlpI GC'TnA_GC
Cuts at: 0 416 421 939
Size: 416 5 518
Fragments arranged by size:
518 416 5
Bme1580I G_kGCm'C
Cuts at: 0 515 764 939
Size: 515 249 175
Fragments arranged by size:
515 249 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 327 939
Size: 327 612
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 461 939
Size: 460 1 478
Fragments arranged by size:
478 460 1
Bpu10I CC'TnA_GC
Cuts at: 0 67 939
Size: 67 872
BsaHI Gr'CG_yC
Cuts at: 0 705 939
Size: 705 234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 719 749 939
Size: 719 30 190
Fragments arranged by size:
719 190 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 213 637 939
Size: 213 424 302
Fragments arranged by size:
424 302 213
BseYI C'CCAG_C
Cuts at: 0 117 939
Size: 117 822
BsiHKAI G_wGCw'C
Cuts at: 0 407 939
Size: 407 532
BsmI GAATG_Cn'
Cuts at: 0 911 939
Size: 911 28
Bsp1286I G_dGCh'C
Cuts at: 0 407 474 515 732 764 939
Size: 407 67 41 217 32 175
Fragments arranged by size:
407 217 175 67 41 32
BspHI T'CATG_A
Cuts at: 0 245 407 939
Size: 245 162 532
Fragments arranged by size:
532 245 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 939
Size: 134 805
BsrGI T'GTAC_A
Cuts at: 0 179 939
Size: 179 760
BstAPI GCAn_nnn'nTGC
Cuts at: 0 335 939
Size: 335 604
BstEII G'GTnAC_C
Cuts at: 0 744 939
Size: 744 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 522 939
Size: 522 417
BstYI r'GATC_y
Cuts at: 0 457 939
Size: 457 482
Bsu36I CC'TnA_GG
Cuts at: 0 781 939
Size: 781 158
BtgI C'CryG_G
Cuts at: 0 465 740 830 939
Size: 465 275 90 109
Fragments arranged by size:
465 275 109 90
BtsI GCAGTG_nn'
Cuts at: 0 557 939
Size: 557 382
DraIII CAC_nnn'GTG
Cuts at: 0 335 939
Size: 335 604
DrdI GACnn_nn'nnGTC
Cuts at: 0 580 939
Size: 580 359
EaeI y'GGCC_r
Cuts at: 0 833 939
Size: 833 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 939
Size: 484 455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 461 939
Size: 460 1 478
Fragments arranged by size:
478 460 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 363 939
Size: 363 576
EcoRI G'AATT_C
Cuts at: 0 47 939
Size: 47 892
HaeII r_GCGC'y
Cuts at: 0 428 708 939
Size: 428 280 231
Fragments arranged by size:
428 280 231
HincII GTy'rAC
Cuts at: 0 26 585 939
Size: 26 559 354
Fragments arranged by size:
559 354 26
KasI G'GCGC_C
Cuts at: 0 704 939
Size: 704 235
KpnI G_GTAC'C
Cuts at: 0 372 939
Size: 372 567
MslI CAynn'nnrTG
Cuts at: 0 211 352 613 939
Size: 211 141 261 326
Fragments arranged by size:
326 261 211 141
MspA1I CmG'CkG
Cuts at: 0 117 694 817 939
Size: 117 577 123 122
Fragments arranged by size:
577 123 122 117
NarI GG'CG_CC
Cuts at: 0 705 939
Size: 705 234
NcoI C'CATG_G
Cuts at: 0 465 939
Size: 465 474
PflMI CCAn_nnn'nTGG
Cuts at: 0 286 939
Size: 286 653
PspOMI G'GGCC_C
Cuts at: 0 511 939
Size: 511 428
PstI C_TGCA'G
Cuts at: 0 552 939
Size: 552 387
PvuII CAG'CTG
Cuts at: 0 117 694 817 939
Size: 117 577 123 122
Fragments arranged by size:
577 123 122 117
SfcI C'TryA_G
Cuts at: 0 548 939
Size: 548 391
SfoI GGC'GCC
Cuts at: 0 706 939
Size: 706 233
StuI AGG'CCT
Cuts at: 0 786 939
Size: 786 153
StyI C'CwwG_G
Cuts at: 0 157 465 939
Size: 157 308 474
Fragments arranged by size:
474 308 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 346 939
Size: 346 593
TatI w'GTAC_w
Cuts at: 0 179 752 939
Size: 179 573 187
Fragments arranged by size:
573 187 179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 694 801 939
Size: 694 107 138
Fragments arranged by size:
694 138 107
Enzymes that do cut:
AccI Acc65I AlwNI ApaI ApoI BamHI BanI BanII
BbeI BciVI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EciI Eco57MI EcoNI EcoRI
HaeII HincII KasI KpnI MslI MspA1I NarI NcoI
PflMI PspOMI PstI PvuII SfcI SfoI StuI StyI
TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BbvCI BcgI BclI BfrBI
BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI
DraI EagI EarI EcoICRI EcoO109I EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI MfeI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI