Restriction map for
pF1KE0299
(Linear) (Six-base) MAPSORT of: KIEE0299.seq Check: 68 from: 1 to: 15429
KIEE0299.orf
With 182 enzymes: SgfI *
January 27, 2010 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1230 2976 3387 15429
Size: 1230 1746 411 12042
Fragments arranged by size:
12042 1746 1230 411
AatII G_ACGT'C
Cuts at: 0 4878 15429
Size: 4878 10551
AccI GT'mk_AC
Cuts at: 0 2979 8743 11098 12504 15429
Size: 2979 5764 2355 1406 2925
Fragments arranged by size:
5764 2979 2925 2355 1406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 673 2002 2132 2381 2799 3599 3665
Size: 629 44 1329 130 249 418 800 66
Cuts at: 3665 3686 3707 3749 3836 4691 4736 5144 5303
Size: 21 21 42 87 855 45 408 159
Cuts at: 5303 6491 6863 7628 7886 8036 9065 9071 9260
Size: 1188 372 765 258 150 1029 6 189
Cuts at: 9260 9599 9638 10064 10079 12986 13097 13220 15026
Size: 339 39 426 15 2907 111 123 1806
Cuts at: 15026 15271 15429
Size: 245 158
Fragments arranged by size:
2907 1806 1329 1188 1029 855 800 765
629 426 418 408 372 339 258 249
245 189 159 158 150 130 123 111
87 66 45 44 42 39 21 21
15 6
AfeI AGC'GCT
Cuts at: 0 9691 15429
Size: 9691 5738
AflIII A'CryG_T
Cuts at: 0 5888 11501 14277 15429
Size: 5888 5613 2776 1152
Fragments arranged by size:
5888 5613 2776 1152
AhdI GACnn_n'nnGTC
Cuts at: 0 7100 9131 15429
Size: 7100 2031 6298
Fragments arranged by size:
7100 6298 2031
AleI CACnn'nnGTG
Cuts at: 0 9343 15429
Size: 9343 6086
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2201 2233 9966 9998 13934 13966 15429
Size: 2201 32 7733 32 3936 32 1463
Fragments arranged by size:
7733 3936 2201 1463 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 64 195 544 1687 2395 2968 3013 4714
Size: 64 131 349 1143 708 573 45 1701
Cuts at: 4714 6856 9007 9169 9472 9921 10513 10669 13789
Size: 2142 2151 162 303 449 592 156 3120
Cuts at: 13789 13806 14743 15004 15429
Size: 17 937 261 425
Fragments arranged by size:
3120 2151 2142 1701 1143 937 708 592
573 449 425 349 303 261 162 156
131 64 45 17
ApaI G_GGCC'C
Cuts at: 0 2886 15429
Size: 2886 12543
ApoI r'AATT_y
Cuts at: 0 514 2829 3551 4127 6455 6655 6674 7632
Size: 514 2315 722 576 2328 200 19 958
Cuts at: 7632 7889 8994 9725 11590 12394 12607 12763 13071
Size: 257 1105 731 1865 804 213 156 308
Cuts at: 13071 13682 13794 14093 15089 15118 15429
Size: 611 112 299 996 29 311
Fragments arranged by size:
2328 2315 1865 1105 996 958 804 731
722 611 576 514 311 308 299 257
213 200 156 112 29 19
AvaI C'yCGr_G
Cuts at: 0 5232 13235 13572 14165 14844 15429
Size: 5232 8003 337 593 679 585
Fragments arranged by size:
8003 5232 679 593 585 337
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5183 5216 6128 6161 8345 8378 10400 10433
Size: 5183 33 912 33 2184 33 2022 33
Cuts at: 10433 15429
Size: 4996
Fragments arranged by size:
5183 4996 2184 2022 912 33 33 33
33
BamHI G'GATC_C
Cuts at: 0 681 3213 12351 13975 15429
Size: 681 2532 9138 1624 1454
Fragments arranged by size:
9138 2532 1624 1454 681
BanI G'GyrC_C
Cuts at: 0 1296 2088 15429
Size: 1296 792 13341
Fragments arranged by size:
13341 1296 792
BanII G_rGCy'C
Cuts at: 0 35 94 1935 2886 3029 5015 5448 5552
Size: 35 59 1841 951 143 1986 433 104
Cuts at: 5552 8501 9662 11088 11151 11918 15429
Size: 2949 1161 1426 63 767 3511
Fragments arranged by size:
3511 2949 1986 1841 1426 1161 951 767
433 143 104 63 59 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 297 1990 3737 4637 4878 4901 5891 7694
Size: 297 1693 1747 900 241 23 990 1803
Cuts at: 7694 12293 12344 15067 15429
Size: 4599 51 2723 362
Fragments arranged by size:
4599 2723 1803 1747 1693 990 900 362
297 241 51 23
BbvCI CC'TCA_GC
Cuts at: 0 1617 5741 7550 9698 11486 15429
Size: 1617 4124 1809 2148 1788 3943
Fragments arranged by size:
4124 3943 2148 1809 1788 1617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1628 1662 9919 9953 13008 13042 15429
Size: 1628 34 8257 34 3055 34 2387
Fragments arranged by size:
8257 3055 2387 1628 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 8504 8673 10180 10401 14480 15429
Size: 8504 169 1507 221 4079 949
Fragments arranged by size:
8504 4079 1507 949 221 169
BclI T'GATC_A
Cuts at: 0 3987 6777 7020 12117 14499 15132 15194 15429
Size: 3987 2790 243 5097 2382 633 62 235
Fragments arranged by size:
5097 3987 2790 2382 633 243 235 62
BfrBI ATG'CAT
Cuts at: 0 832 5506 5766 12994 15429
Size: 832 4674 260 7228 2435
Fragments arranged by size:
7228 4674 2435 832 260
BglI GCCn_nnn'nGGC
Cuts at: 0 61 1008 15429
Size: 61 947 14421
Fragments arranged by size:
14421 947 61
BglII A'GATC_T
Cuts at: 0 5557 7833 9072 9944 15429
Size: 5557 2276 1239 872 5485
Fragments arranged by size:
5557 5485 2276 1239 872
BlpI GC'TnA_GC
Cuts at: 0 142 998 1088 1106 1118 1175 1485 1605
Size: 142 856 90 18 12 57 310 120
Cuts at: 1605 1652 3113 8495 9863 11390 12819 15429
Size: 47 1461 5382 1368 1527 1429 2610
Fragments arranged by size:
5382 2610 1527 1461 1429 1368 856 310
142 120 90 57 47 18 12
Bme1580I G_kGCm'C
Cuts at: 0 2886 10626 11236 12799 15429
Size: 2886 7740 610 1563 2630
Fragments arranged by size:
7740 2886 2630 1563 610
BmrI ACTGGGnnnn_n'
Cuts at: 0 1048 1136 2501 2792 6892 6932 9219 9828
Size: 1048 88 1365 291 4100 40 2287 609
Cuts at: 9828 12802 15429
Size: 2974 2627
Fragments arranged by size:
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88 40
BmtI G_CTAG'C
Cuts at: 0 80 15429
Size: 80 15349
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 113 10493 11488 15429
Size: 113 10380 995 3941
Fragments arranged by size:
10380 3941 995 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 737 926 1067 1097 1184 1205 1271
Size: 86 651 189 141 30 87 21 66
Cuts at: 1271 1301 1937 3166 4997 6013 7109 7325 7946
Size: 30 636 1229 1831 1016 1096 216 621
Cuts at: 7946 8081 8561 9691 9943 10268 10527 11165 11275
Size: 135 480 1130 252 325 259 638 110
Cuts at: 11275 11458 12681 12864 13870 14383 15429
Size: 183 1223 183 1006 513 1046
Fragments arranged by size:
1831 1229 1223 1130 1096 1046 1016 1006
651 638 636 621 513 480 325 259
252 216 189 183 183 141 135 110
87 86 66 30 30 21
Bpu10I CC'TnA_GC
Cuts at: 0 659 1292 1617 2093 5741 7463 7508 7550
Size: 659 633 325 476 3648 1722 45 42
Cuts at: 7550 9698 10174 11486 13865 15429
Size: 2148 476 1312 2379 1564
Fragments arranged by size:
3648 2379 2148 1722 1564 1312 659 633
476 476 325 45 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2753 3785 4029 4150 4250 9901 11801 12054
Size: 2753 1032 244 121 100 5651 1900 253
Cuts at: 12054 12251 12941 14332 15429
Size: 197 690 1391 1097
Fragments arranged by size:
5651 2753 1900 1391 1097 1032 690 253
244 197 121 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 331 2767 7087 7381 8074 12269 12626 15429
Size: 331 2436 4320 294 693 4195 357 2803
Fragments arranged by size:
4320 4195 2803 2436 693 357 331 294
BsaAI yAC'GTr
Cuts at: 0 5891 15429
Size: 5891 9538
BsaBI GATnn'nnATC
Cuts at: 0 10433 12116 14394 15429
Size: 10433 1683 2278 1035
Fragments arranged by size:
10433 2278 1683 1035
BsaHI Gr'CG_yC
Cuts at: 0 309 4875 15429
Size: 309 4566 10554
Fragments arranged by size:
10554 4566 309
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 774 804 863 893 1065 1095 1299 1329
Size: 774 30 59 30 172 30 204 30
Cuts at: 1329 1389 1419 1701 1731 1733 1763 2361 2391
Size: 60 30 282 30 2 30 598 30
Cuts at: 2391 7431 7461 8079 8109 8607 8637 9769 9799
Size: 5040 30 618 30 498 30 1132 30
Cuts at: 9799 9966 9996 10241 10271 14126 14156 14784 14814
Size: 167 30 245 30 3855 30 628 30
Cuts at: 14814 15429
Size: 615
Fragments arranged by size:
5040 3855 1132 774 628 618 615 598
498 282 245 204 172 167 60 59
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 83 86 166 171 396 782 2369 2756
Size: 83 3 80 5 225 386 1587 387
Cuts at: 2756 4411 4669 4994 4997 5560 5758 7205 7214
Size: 1655 258 325 3 563 198 1447 9
Cuts at: 7214 7229 7232 7235 7238 7256 7439 9790 9940
Size: 15 3 3 3 18 183 2351 150
Cuts at: 9940 11206 14832 15429
Size: 1266 3626 597
Fragments arranged by size:
3626 2351 1655 1587 1447 1266 597 563
387 386 325 258 225 198 183 150
83 80 18 15 9 5 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 993 1235 1382 1425 1455 1545 1635 1970
Size: 993 242 147 43 30 90 90 335
Cuts at: 1970 3006 9169 10998 13799 13990 15429
Size: 1036 6163 1829 2801 191 1439
Fragments arranged by size:
6163 2801 1829 1439 1036 993 335 242
191 147 90 90 43 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1973 2048 3365 7427 8465 8844 15429
Size: 1973 75 1317 4062 1038 379 6585
Fragments arranged by size:
6585 4062 1973 1317 1038 379 75
BsiEI CG_ry'CG
Cuts at: 0 56 116 221 15429
Size: 56 60 105 15208
Fragments arranged by size:
15208 105 60 56
BsiHKAI G_wGCw'C
Cuts at: 0 443 1935 5015 5448 5552 9603 9662 10180
Size: 443 1492 3080 433 104 4051 59 518
Cuts at: 10180 15429
Size: 5249
Fragments arranged by size:
5249 4051 3080 1492 518 443 433 104
59
BsmI GAATG_Cn'
Cuts at: 0 3247 6305 9039 13258 14250 14783 15429
Size: 3247 3058 2734 4219 992 533 646
Fragments arranged by size:
4219 3247 3058 2734 992 646 533
BsmBI CGTCTCn'nnnn_
Cuts at: 0 6347 9523 9847 12241 15429
Size: 6347 3176 324 2394 3188
Fragments arranged by size:
6347 3188 3176 2394 324
Bsp1286I G_dGCh'C
Cuts at: 0 35 94 443 1935 2886 3029 5015 5448
Size: 35 59 349 1492 951 143 1986 433
Cuts at: 5448 5552 8501 9603 9662 10180 10626 11088 11151
Size: 104 2949 1102 59 518 446 462 63
Cuts at: 11151 11236 11918 12799 15429
Size: 85 682 881 2630
Fragments arranged by size:
2949 2630 1986 1492 1102 951 881 682
518 462 446 433 349 143 104 85
63 59 59 35
BspHI T'CATG_A
Cuts at: 0 9839 12207 13815 15429
Size: 9839 2368 1608 1614
Fragments arranged by size:
9839 2368 1614 1608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1230 2478 2976 3387 7122 9402 12854 14595
Size: 1230 1248 498 411 3735 2280 3452 1741
Cuts at: 14595 15429
Size: 834
Fragments arranged by size:
3735 3452 2280 1741 1248 1230 834 498
411
BsrBI CCG'CTC
Cuts at: 0 9825 15429
Size: 9825 5604
BsrDI GCAATG_nn'
Cuts at: 0 7750 8462 9461 10154 13192 15429
Size: 7750 712 999 693 3038 2237
Fragments arranged by size:
7750 3038 2237 999 712 693
BsrFI r'CCGG_y
Cuts at: 0 115 12260 14810 14836 15429
Size: 115 12145 2550 26 593
Fragments arranged by size:
12145 2550 593 115 26
BsrGI T'GTAC_A
Cuts at: 0 10335 15429
Size: 10335 5094
BssSI C'ACGA_G
Cuts at: 0 10478 10625 11276 15429
Size: 10478 147 651 4153
Fragments arranged by size:
10478 4153 651 147
BstAPI GCAn_nnn'nTGC
Cuts at: 0 195 372 3242 6219 8452 14743 15429
Size: 195 177 2870 2977 2233 6291 686
Fragments arranged by size:
6291 2977 2870 2233 686 195 177
BstBI TT'CG_AA
Cuts at: 0 4668 11343 12243 15429
Size: 4668 6675 900 3186
Fragments arranged by size:
6675 4668 3186 900
BstEII G'GTnAC_C
Cuts at: 0 2621 3564 15429
Size: 2621 943 11865
Fragments arranged by size:
11865 2621 943
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1390 3144 7836 11271 15429
Size: 1390 1754 4692 3435 4158
Fragments arranged by size:
4692 4158 3435 1754 1390
BstYI r'GATC_y
Cuts at: 0 681 1684 1944 2076 3213 5146 5557 6003
Size: 681 1003 260 132 1137 1933 411 446
Cuts at: 6003 6235 7833 8694 9072 9887 9944 11252 12216
Size: 232 1598 861 378 815 57 1308 964
Cuts at: 12216 12351 12772 13942 13975 15369 15429
Size: 135 421 1170 33 1394 60
Fragments arranged by size:
1933 1598 1394 1308 1170 1137 1003 964
861 815 681 446 421 411 378 260
232 135 132 60 57 33
BstZ17I GTA'TAC
Cuts at: 0 2980 11099 15429
Size: 2980 8119 4330
Fragments arranged by size:
8119 4330 2980
Bsu36I CC'TnA_GG
Cuts at: 0 1005 2171 2693 6074 8891 11189 15429
Size: 1005 1166 522 3381 2817 2298 4240
Fragments arranged by size:
4240 3381 2817 2298 1166 1005 522
BtgI C'CryG_G
Cuts at: 0 6284 11890 14336 14571 15429
Size: 6284 5606 2446 235 858
Fragments arranged by size:
6284 5606 2446 858 235
BtsI GCAGTG_nn'
Cuts at: 0 889 2018 2840 3452 4617 6369 8594 9386
Size: 889 1129 822 612 1165 1752 2225 792
Cuts at: 9386 14868 14897 15429
Size: 5482 29 532
Fragments arranged by size:
5482 2225 1752 1165 1129 889 822 792
612 532 29
ClaI AT'CG_AT
Cuts at: 0 8934 15429
Size: 8934 6495
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2277 2312 13814 13849 15429
Size: 2277 35 11502 35 1580
Fragments arranged by size:
11502 2277 1580 35 35
DraI TTT'AAA
Cuts at: 0 431 3320 4437 5001 10314 15429
Size: 431 2889 1117 564 5313 5115
Fragments arranged by size:
5313 5115 2889 1117 564 431
DraIII CAC_nnn'GTG
Cuts at: 0 1835 2872 4123 15429
Size: 1835 1037 1251 11306
Fragments arranged by size:
11306 1835 1251 1037
DrdI GACnn_nn'nnGTC
Cuts at: 0 12261 13421 15429
Size: 12261 1160 2008
Fragments arranged by size:
12261 2008 1160
EaeI y'GGCC_r
Cuts at: 0 53 113 218 2655 8369 13104 13121 15429
Size: 53 60 105 2437 5714 4735 17 2308
Fragments arranged by size:
5714 4735 2437 2308 105 60 53 17
EagI C'GGCC_G
Cuts at: 0 53 113 218 15429
Size: 53 60 105 15211
Fragments arranged by size:
15211 105 60 53
EarI CTCTTCn'nnn_
Cuts at: 0 143 431 2357 3581 3626 4388 4512 4535
Size: 143 288 1926 1224 45 762 124 23
Cuts at: 4535 4646 5078 5513 5958 6202 6594 7160 7974
Size: 111 432 435 445 244 392 566 814
Cuts at: 7974 8805 8891 9815 11679 11690 12942 15290 15429
Size: 831 86 924 1864 11 1252 2348 139
Fragments arranged by size:
2348 1926 1864 1252 1224 924 831 814
762 566 445 435 432 392 288 244
143 139 124 111 86 45 23 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 142 12346 15429
Size: 142 12204 3083
Fragments arranged by size:
12204 3083 142
EcoICRI GAG'CTC
Cuts at: 0 1933 5013 5446 5550 9660 15429
Size: 1933 3080 433 104 4110 5769
Fragments arranged by size:
5769 4110 3080 1933 433 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 629 673 737 926 1067 1097 1184
Size: 86 543 44 64 189 141 30 87
Cuts at: 1184 1205 1271 1301 1937 2002 2132 2381 2799
Size: 21 66 30 636 65 130 249 418
Cuts at: 2799 3166 3599 3665 3686 3707 3749 3836 4691
Size: 367 433 66 21 21 42 87 855
Cuts at: 4691 4736 4997 5144 5303 6013 6491 6863 7109
Size: 45 261 147 159 710 478 372 246
Cuts at: 7109 7325 7628 7886 7946 8036 8081 8561 9065
Size: 216 303 258 60 90 45 480 504
Cuts at: 9065 9071 9260 9599 9638 9691 9943 10064 10079
Size: 6 189 339 39 53 252 121 15
Cuts at: 10079 10268 10527 11165 11275 11458 12681 12864 12986
Size: 189 259 638 110 183 1223 183 122
Cuts at: 12986 13097 13220 13870 14383 15026 15271 15429
Size: 111 123 650 513 643 245 158
Fragments arranged by size:
1223 855 710 650 643 638 636 543
513 504 480 478 433 418 372 367
339 303 261 259 258 252 249 246
245 216 189 189 189 183 183 159
158 147 141 130 123 122 121 111
110 90 87 87 86 66 66 65
64 60 53 45 45 44 42 39
30 30 21 21 21 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 1334 1742 11440 14413 15429
Size: 472 862 408 9698 2973 1016
Fragments arranged by size:
9698 2973 1016 862 472 408
EcoO109I rG'GnC_Cy
Cuts at: 0 670 778 1363 2238 2882 2883 3391 6252
Size: 670 108 585 875 644 1 508 2861
Cuts at: 6252 6894 9220 11433 13960 14009 15429
Size: 642 2326 2213 2527 49 1420
Fragments arranged by size:
2861 2527 2326 2213 1420 875 670 644
642 585 508 108 49 1
EcoRI G'AATT_C
Cuts at: 0 11590 13071 13682 15429
Size: 11590 1481 611 1747
Fragments arranged by size:
11590 1747 1481 611
EcoRV GAT'ATC
Cuts at: 0 536 9483 10574 15429
Size: 536 8947 1091 4855
Fragments arranged by size:
8947 4855 1091 536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3328 3841 4223 4541 5455 6752 7081 7208
Size: 3328 513 382 318 914 1297 329 127
Cuts at: 7208 11377 12944 14224 15429
Size: 4169 1567 1280 1205
Fragments arranged by size:
4169 3328 1567 1297 1280 1205 914 513
382 329 318 127
HaeII r_GCGC'y
Cuts at: 0 9693 15429
Size: 9693 5736
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 520 552 1188 1220 1335 1367
Size: 430 32 58 32 636 32 115 32
Cuts at: 1367 2170 2202 2284 2316 2508 2540 2795 2827
Size: 803 32 82 32 192 32 255 32
Cuts at: 2827 3085 3117 4018 4050 4093 4125 4276 4308
Size: 258 32 901 32 43 32 151 32
Cuts at: 4308 5680 5712 6793 6825 7086 7118 9117 9149
Size: 1372 32 1081 32 261 32 1999 32
Cuts at: 9149 9769 9801 9846 9878 10241 10273 10284 10316
Size: 620 32 45 32 363 32 11 32
Cuts at: 10316 11488 11520 12405 12437 13754 13786 14213 14245
Size: 1172 32 885 32 1317 32 427 32
Cuts at: 14245 15429
Size: 1184
Fragments arranged by size:
1999 1372 1317 1184 1172 1081 901 885
803 636 620 430 427 363 261 258
255 192 151 115 82 58 45 43
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
11
HincII GTy'rAC
Cuts at: 0 480 2538 7328 13426 15390 15429
Size: 480 2058 4790 6098 1964 39
Fragments arranged by size:
6098 4790 2058 1964 480 39
HindIII A'AGCT_T
Cuts at: 0 3611 3823 5786 6054 6953 12367 13645 15429
Size: 3611 212 1963 268 899 5414 1278 1784
Fragments arranged by size:
5414 3611 1963 1784 1278 899 268 212
HpaI GTT'AAC
Cuts at: 0 480 7328 15429
Size: 480 6848 8101
Fragments arranged by size:
8101 6848 480
MfeI C'AATT_G
Cuts at: 0 5006 10094 13058 15429
Size: 5006 5088 2964 2371
Fragments arranged by size:
5088 5006 2964 2371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1170 1255 3070 4168 5536 6956 7582
Size: 239 931 85 1815 1098 1368 1420 626
Cuts at: 7582 7718 7798 8337 8496 9313 9752 9915 10575
Size: 136 80 539 159 817 439 163 660
Cuts at: 10575 11086 11668 13302 14423 15429
Size: 511 582 1634 1121 1006
Fragments arranged by size:
1815 1634 1420 1368 1121 1098 1006 931
817 660 626 582 539 511 439 239
163 159 136 85 80
MscI TGG'CCA
Cuts at: 0 2657 8371 13106 13123 15429
Size: 2657 5714 4735 17 2306
Fragments arranged by size:
5714 4735 2657 2306 17
MslI CAynn'nnrTG
Cuts at: 0 4684 9343 12320 15429
Size: 4684 4659 2977 3109
Fragments arranged by size:
4684 4659 3109 2977
MspA1I CmG'CkG
Cuts at: 0 61 141 172 223 2653 3013 3112 5665
Size: 61 80 31 51 2430 360 99 2553
Cuts at: 5665 5797 7396 7882 9897 9921 10084 10836 12181
Size: 132 1599 486 2015 24 163 752 1345
Cuts at: 12181 13135 13914 15429
Size: 954 779 1515
Fragments arranged by size:
2553 2430 2015 1599 1515 1345 954 779
752 486 360 163 132 99 80 61
51 31 24
NaeI GCC'GGC
Cuts at: 0 117 15429
Size: 117 15312
NcoI C'CATG_G
Cuts at: 0 6284 14336 14571 15429
Size: 6284 8052 235 858
Fragments arranged by size:
8052 6284 858 235
NdeI CA'TA_TG
Cuts at: 0 8946 13704 15429
Size: 8946 4758 1725
Fragments arranged by size:
8946 4758 1725
NgoMIV G'CCGG_C
Cuts at: 0 115 15429
Size: 115 15314
NheI G'CTAG_C
Cuts at: 0 76 15429
Size: 76 15353
NotI GC'GGCC_GC
Cuts at: 0 53 15429
Size: 53 15376
NsiI A_TGCA'T
Cuts at: 0 834 5508 5768 12996 15429
Size: 834 4674 260 7228 2433
Fragments arranged by size:
7228 4674 2433 834 260
NspI r_CATG'y
Cuts at: 0 1801 1829 5164 8399 8455 8494 8587 11505
Size: 1801 28 3335 3235 56 39 93 2918
Cuts at: 11505 13285 14281 14604 15429
Size: 1780 996 323 825
Fragments arranged by size:
3335 3235 2918 1801 1780 996 825 323
93 56 39 28
PasI CC'CwG_GG
Cuts at: 0 304 8667 13346 13852 13996 15429
Size: 304 8363 4679 506 144 1433
Fragments arranged by size:
8363 4679 1433 506 304 144
PciI A'CATG_T
Cuts at: 0 11501 14277 15429
Size: 11501 2776 1152
Fragments arranged by size:
11501 2776 1152
PflMI CCAn_nnn'nTGG
Cuts at: 0 931 1242 6760 9921 11793 13113 13806 15429
Size: 931 311 5518 3161 1872 1320 693 1623
Fragments arranged by size:
5518 3161 1872 1623 1320 931 693 311
PfoI T'CCnGG_A
Cuts at: 0 930 1182 9662 11242 11291 15429
Size: 930 252 8480 1580 49 4138
Fragments arranged by size:
8480 4138 1580 930 252 49
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 9966 9998 15429
Size: 9966 32 5431
Fragments arranged by size:
9966 5431 32
PpuMI rG'GwC_Cy
Cuts at: 0 670 778 1363 3391 6894 9220 13960 14009
Size: 670 108 585 2028 3503 2326 4740 49
Cuts at: 14009 15429
Size: 1420
Fragments arranged by size:
4740 3503 2326 2028 1420 670 585 108
49
PshAI GACnn'nnGTC
Cuts at: 0 8389 15429
Size: 8389 7040
PsiI TTA'TAA
Cuts at: 0 5651 7655 8326 8837 9436 14324 15429
Size: 5651 2004 671 511 599 4888 1105
Fragments arranged by size:
5651 4888 2004 1105 671 599 511
PspOMI G'GGCC_C
Cuts at: 0 2882 15429
Size: 2882 12547
PspXI vC'TCGA_Gb
Cuts at: 0 13235 15429
Size: 13235 2194
PstI C_TGCA'G
Cuts at: 0 1703 2847 5046 5538 9785 11260 12096 12830
Size: 1703 1144 2199 492 4247 1475 836 734
Cuts at: 12830 13134 13161 14820 14904 15276 15429
Size: 304 27 1659 84 372 153
Fragments arranged by size:
4247 2199 1703 1659 1475 1144 836 734
492 372 304 153 84 27
PvuII CAG'CTG
Cuts at: 0 141 3013 3112 5665 5797 7396 7882 9897
Size: 141 2872 99 2553 132 1599 486 2015
Cuts at: 9897 9921 10084 10836 13135 15429
Size: 24 163 752 2299 2294
Fragments arranged by size:
2872 2553 2299 2294 2015 1599 752 486
163 141 132 99 24
SacI G_AGCT'C
Cuts at: 0 1935 5015 5448 5552 9662 15429
Size: 1935 3080 433 104 4110 5767
Fragments arranged by size:
5767 4110 3080 1935 433 104
SanDI GG'GwC_CC
Cuts at: 0 14009 15429
Size: 14009 1420
SapI GCTCTTCn'nnn_
Cuts at: 0 431 3626 5078 6594 8805 9815 11690 12942
Size: 431 3195 1452 1516 2211 1010 1875 1252
Cuts at: 12942 15429
Size: 2487
Fragments arranged by size:
3195 2487 2211 1875 1516 1452 1252 1010
431
SbfI CC_TGCA'GG
Cuts at: 0 15276 15429
Size: 15276 153
ScaI AGT'ACT
Cuts at: 0 4764 10970 11671 15429
Size: 4764 6206 701 3758
Fragments arranged by size:
6206 4764 3758 701
SexAI A'CCwGG_T
Cuts at: 0 321 672 8522 10164 15429
Size: 321 351 7850 1642 5265
Fragments arranged by size:
7850 5265 1642 351 321
SfcI C'TryA_G
Cuts at: 0 344 1279 1699 1851 2843 3419 5042 5534
Size: 344 935 420 152 992 576 1623 492
Cuts at: 5534 6152 6827 7349 7862 7883 8420 8534 9095
Size: 618 675 522 513 21 537 114 561
Cuts at: 9095 9128 9781 9995 11256 12092 12465 12826 13130
Size: 33 653 214 1261 836 373 361 304
Cuts at: 13130 13157 13900 14816 14900 15272 15429
Size: 27 743 916 84 372 157
Fragments arranged by size:
1623 1261 992 935 916 836 743 675
653 618 576 561 537 522 513 492
420 373 372 361 344 304 214 157
152 114 84 33 27 21
SmaI CCC'GGG
Cuts at: 0 13574 15429
Size: 13574 1855
SmlI C'TyrA_G
Cuts at: 0 2768 3800 4044 4165 4265 9880 11816 12033
Size: 2768 1032 244 121 100 5615 1936 217
Cuts at: 12033 12266 12956 13235 14165 14347 15429
Size: 233 690 279 930 182 1082
Fragments arranged by size:
5615 2768 1936 1082 1032 930 690 279
244 233 217 182 121 100
SpeI A'CTAG_T
Cuts at: 0 2115 4591 11634 15429
Size: 2115 2476 7043 3795
Fragments arranged by size:
7043 3795 2476 2115
SphI G_CATG'C
Cuts at: 0 5164 8494 15429
Size: 5164 3330 6935
Fragments arranged by size:
6935 5164 3330
SspI AAT'ATT
Cuts at: 0 5038 5575 12747 15108 15174 15429
Size: 5038 537 7172 2361 66 255
Fragments arranged by size:
7172 5038 2361 537 255 66
StuI AGG'CCT
Cuts at: 0 840 1748 7506 7604 15429
Size: 840 908 5758 98 7825
Fragments arranged by size:
7825 5758 908 840 98
StyI C'CwwG_G
Cuts at: 0 200 2084 2153 4071 4373 4638 6284 7814
Size: 200 1884 69 1918 302 265 1646 1530
Cuts at: 7814 10113 10637 14336 14409 14461 14571 15429
Size: 2299 524 3699 73 52 110 858
Fragments arranged by size:
3699 2299 1918 1884 1646 1530 858 524
302 265 200 110 73 69 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 660 2952 6558 6609 7040 8431 10776 10866
Size: 660 2292 3606 51 431 1391 2345 90
Cuts at: 10866 10893 11715 13397 13546 15429
Size: 27 822 1682 149 1883
Fragments arranged by size:
3606 2345 2292 1883 1682 1391 822 660
431 149 90 51 27
TatI w'GTAC_w
Cuts at: 0 2346 3804 4240 4273 4762 5020 5637 7435
Size: 2346 1458 436 33 489 258 617 1798
Cuts at: 7435 8050 8407 8701 10151 10335 10830 10968 11669
Size: 615 357 294 1450 184 495 138 701
Cuts at: 11669 12017 14258 15429
Size: 348 2241 1171
Fragments arranged by size:
2346 2241 1798 1458 1450 1171 701 617
615 495 489 436 357 348 294 258
184 138 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1733 1765 6362 6394 8502 8534 8607 8639
Size: 1733 32 4597 32 2108 32 73 32
Cuts at: 8639 9694 9726 10154 10186 10464 10479 10496 10511
Size: 1055 32 428 32 278 15 17 15
Cuts at: 10511 10716 10748 15429
Size: 205 32 4681
Fragments arranged by size:
4681 4597 2108 1733 1055 428 278 205
73 32 32 32 32 32 32 32
17 15 15
Tth111I GACn'n_nGTC
Cuts at: 0 1128 6276 8524 13373 15429
Size: 1128 5148 2248 4849 2056
Fragments arranged by size:
5148 4849 2248 2056 1128
XbaI T'CTAG_A
Cuts at: 0 7383 8112 12307 12972 13411 15429
Size: 7383 729 4195 665 439 2018
Fragments arranged by size:
7383 4195 2018 729 665 439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1684 6256 6603 7812 10833 14635 15429
Size: 1684 4572 347 1209 3021 3802 794
Fragments arranged by size:
4572 3802 3021 1684 1209 794 347
XhoI C'TCGA_G
Cuts at: 0 13235 14165 15429
Size: 13235 930 1264
Fragments arranged by size:
13235 1264 930
XmaI C'CCGG_G
Cuts at: 0 13572 15429
Size: 13572 1857
XmnI GAAnn'nnTTC
Cuts at: 0 1374 3551 5403 7188 9572 9978 11942 13712
Size: 1374 2177 1852 1785 2384 406 1964 1770
Cuts at: 13712 15251 15429
Size: 1539 178
Fragments arranged by size:
2384 2177 1964 1852 1785 1770 1539 1374
406 178
ZraI GAC'GTC
Cuts at: 0 4876 15429
Size: 4876 10553
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuII SacI SanDI SapI SbfI
ScaI SexAI SfcI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AlfI ApaLI AscI AseI
AsiSI AvrII BbeI BmgBI BsaWI BsiWI BspEI BssHII
BtgZI FseI FspI FspAI KasI KpnI MluI NarI
NruI PacI PmeI PmlI PsrI PvuI RsrII SacII
SalI SfiI SfoI SgfI SgrAI SnaBI SrfI SwaI