(Linear) (Six-base) MAPSORT of: KIEE0304.seq  Check: 3379  from: 1  to: 1467



KIEE0304.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AccI GT'mk_AC

Cuts at:      0     452    1467 
   Size:        452    1015
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16    1220    1467 
   Size:         16    1204     247

  Fragments arranged by size: 

                1204     247      16
 
AflIII A'CryG_T

Cuts at:      0      98    1467 
   Size:         98    1369
 
AlwNI CAG_nnn'CTG

Cuts at:      0     141    1168    1467 
   Size:        141    1027     299

  Fragments arranged by size: 

                1027     299     141
 
ApoI r'AATT_y

Cuts at:      0     397     907     933    1140    1326    1467 
   Size:        397     510      26     207     186     141

  Fragments arranged by size: 

                 510     397     207     186     141      26
 
AseI AT'TA_AT

Cuts at:      0     833    1403    1467 
   Size:        833     570      64

  Fragments arranged by size: 

                 833     570      64
 
AvrII C'CTAG_G

Cuts at:      0    1225    1467 
   Size:       1225     242
 
BanI G'GyrC_C

Cuts at:      0    1421    1467 
   Size:       1421      46
 
BclI T'GATC_A

Cuts at:      0     815    1467 
   Size:        815     652
 
BfrBI ATG'CAT

Cuts at:      0     414    1467 
   Size:        414    1053
 
BglII A'GATC_T

Cuts at:      0     846    1467 
   Size:        846     621
 
BlpI GC'TnA_GC

Cuts at:      0     596    1467 
   Size:        596     871
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1363    1467 
   Size:       1363     104
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     669    1467 
   Size:        669     798
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     455    1411    1467 
   Size:        202     253     956      56

  Fragments arranged by size: 

                 956     253     202      56
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     313     951    1467 
   Size:        313     638     516

  Fragments arranged by size: 

                 638     516     313
 
BsaBI GATnn'nnATC

Cuts at:      0     482    1467 
   Size:        482     985
 
BsaWI w'CCGG_w

Cuts at:      0     126    1467 
   Size:        126    1341
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      70     100    1467 
   Size:         70      30    1367

  Fragments arranged by size: 

                1367      70      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52    1467 
   Size:         52    1415
 
BseYI C'CCAG_C

Cuts at:      0     226     740    1467 
   Size:        226     514     727

  Fragments arranged by size: 

                 727     514     226
 
BsiHKAI G_wGCw'C

Cuts at:      0     602    1412    1467 
   Size:        602     810      55

  Fragments arranged by size: 

                 810     602      55
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     277    1467 
   Size:        277    1190
 
Bsp1286I G_dGCh'C

Cuts at:      0     602    1412    1467 
   Size:        602     810      55

  Fragments arranged by size: 

                 810     602      55
 
BspHI T'CATG_A

Cuts at:      0     579    1467 
   Size:        579     888
 
BsrDI GCAATG_nn'

Cuts at:      0     513    1467 
   Size:        513     954
 
BstYI r'GATC_y

Cuts at:      0     309     846    1042    1467 
   Size:        309     537     196     425

  Fragments arranged by size: 

                 537     425     309     196
 
BstZ17I GTA'TAC

Cuts at:      0     453    1467 
   Size:        453    1014
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     400    1467 
   Size:        400    1067
 
BtsI GCAGTG_nn'

Cuts at:      0      22    1293    1467 
   Size:         22    1271     174

  Fragments arranged by size: 

                1271     174      22
 
DraI TTT'AAA

Cuts at:      0     854    1467 
   Size:        854     613
 
EaeI y'GGCC_r

Cuts at:      0     701    1467 
   Size:        701     766
 
EarI CTCTTCn'nnn_

Cuts at:      0    1098    1467 
   Size:       1098     369
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      73    1467 
   Size:         73    1394
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     202     455    1220    1411    1467 
   Size:         16     186     253     765     191      56

  Fragments arranged by size: 

                 765     253     191     186      56      16
 
EcoRI G'AATT_C

Cuts at:      0    1326    1467 
   Size:       1326     141
 
EcoRV GAT'ATC

Cuts at:      0    1130    1467 
   Size:       1130     337
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1160    1467 
   Size:       1160     307
 
HaeII r_GCGC'y

Cuts at:      0     504    1467 
   Size:        504     963
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      70     102    1467 
   Size:         70      32    1365

  Fragments arranged by size: 

                1365      70      32
 
MluI A'CGCG_T

Cuts at:      0      98    1467 
   Size:         98    1369
 
MslI CAynn'nnrTG

Cuts at:      0     974    1467 
   Size:        974     493
 
MspA1I CmG'CkG

Cuts at:      0     180    1467 
   Size:        180    1287
 
NdeI CA'TA_TG

Cuts at:      0     410    1467 
   Size:        410    1057
 
NsiI A_TGCA'T

Cuts at:      0     416    1467 
   Size:        416    1051
 
NspI r_CATG'y

Cuts at:      0     346     829    1467 
   Size:        346     483     638

  Fragments arranged by size: 

                 638     483     346
 
PvuII CAG'CTG

Cuts at:      0     180    1467 
   Size:        180    1287
 
StyI C'CwwG_G

Cuts at:      0    1048    1225    1467 
   Size:       1048     177     242

  Fragments arranged by size: 

                1048     242     177
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     392    1467 
   Size:        392    1075
 
TatI w'GTAC_w

Cuts at:      0    1309    1467 
   Size:       1309     158
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      88     120    1467 
   Size:         88      32    1347

  Fragments arranged by size: 

                1347      88      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     610    1189    1467 
   Size:        610     579     278

  Fragments arranged by size: 

                 610     579     278
 
XmnI GAAnn'nnTTC

Cuts at:      0     261    1113    1143    1467 
   Size:        261     852      30     324

  Fragments arranged by size: 

                 852     324     261      30
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AseI    AvrII     BanI
     BclI    BfrBI    BglII     BlpI     BmrI     BplI     BpmI     BsaI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I
    BspHI    BsrDI    BstYI  BstZ17I    BtgZI     BtsI     DraI     EaeI
     EarI     EciI  Eco57MI    EcoRI    EcoRV     FalI    HaeII    Hin4I
     MluI     MslI   MspA1I     NdeI     NsiI     NspI    PvuII     StyI
    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BglI Bme1580I    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI    BsaHI
     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI   Bsu36I
     BtgI     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI
 EcoO109I     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI