Restriction map for
pF1KE0305
(Linear) (Six-base) MAPSORT of: KIEE0305.seq Check: 109 from: 1 to: 1956
KIEE0305.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1675 1956
Size: 1675 281
AccI GT'mk_AC
Cuts at: 0 1551 1956
Size: 1551 405
Acc65I G'GTAC_C
Cuts at: 0 998 1956
Size: 998 958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 352 1956
Size: 324 28 1604
Fragments arranged by size:
1604 324 28
AfeI AGC'GCT
Cuts at: 0 135 418 1956
Size: 135 283 1538
Fragments arranged by size:
1538 283 135
AflIII A'CryG_T
Cuts at: 0 977 1956
Size: 977 979
AhdI GACnn_n'nnGTC
Cuts at: 0 701 1956
Size: 701 1255
AleI CACnn'nnGTG
Cuts at: 0 1768 1956
Size: 1768 188
AlwNI CAG_nnn'CTG
Cuts at: 0 237 1956
Size: 237 1719
ApaI G_GGCC'C
Cuts at: 0 490 586 763 1300 1309 1439 1516 1956
Size: 490 96 177 537 9 130 77 440
Fragments arranged by size:
537 490 440 177 130 96 77 9
AvaI C'yCGr_G
Cuts at: 0 52 120 756 1011 1299 1516 1956
Size: 52 68 636 255 288 217 440
Fragments arranged by size:
636 440 288 255 217 68 52
AvrII C'CTAG_G
Cuts at: 0 1758 1956
Size: 1758 198
BanI G'GyrC_C
Cuts at: 0 998 1522 1762 1956
Size: 998 524 240 194
Fragments arranged by size:
998 524 240 194
BanII G_rGCy'C
Cuts at: 0 290 395 490 502 586 763 1300 1309
Size: 290 105 95 12 84 177 537 9
Cuts at: 1309 1439 1516 1640 1956
Size: 130 77 124 316
Fragments arranged by size:
537 316 290 177 130 124 105 95
84 77 12 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 896 930 1956
Size: 896 34 1026
Fragments arranged by size:
1026 896 34
BglI GCCn_nnn'nGGC
Cuts at: 0 259 658 1304 1367 1520 1956
Size: 259 399 646 63 153 436
Fragments arranged by size:
646 436 399 259 153 63
BlpI GC'TnA_GC
Cuts at: 0 130 1956
Size: 130 1826
Bme1580I G_kGCm'C
Cuts at: 0 490 586 647 763 1065 1300 1309 1439
Size: 490 96 61 116 302 235 9 130
Cuts at: 1439 1484 1516 1525 1956
Size: 45 32 9 431
Fragments arranged by size:
490 431 302 235 130 116 96 61
45 32 9 9
BmgBI CAC'GTC
Cuts at: 0 213 1035 1281 1956
Size: 213 822 246 675
Fragments arranged by size:
822 675 246 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 756 1604 1956
Size: 756 848 352
Fragments arranged by size:
848 756 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 906 1154 1650 1956
Size: 274 632 248 496 306
Fragments arranged by size:
632 496 306 274 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1513 1579 1956
Size: 1513 66 377
Fragments arranged by size:
1513 377 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 1398 1956
Size: 1398 558
BsaAI yAC'GTr
Cuts at: 0 947 980 1956
Size: 947 33 976
Fragments arranged by size:
976 947 33
BsaHI Gr'CG_yC
Cuts at: 0 1571 1625 1956
Size: 1571 54 331
Fragments arranged by size:
1571 331 54
BsaWI w'CCGG_w
Cuts at: 0 395 1956
Size: 395 1561
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1867 1897 1912 1942 1956
Size: 1867 30 15 30 14
Fragments arranged by size:
1867 30 30 15 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 1249 1462 1686 1855 1956
Size: 214 1035 213 224 169 101
Fragments arranged by size:
1035 224 214 213 169 101
BseYI C'CCAG_C
Cuts at: 0 47 206 230 344 446 460 1106 1370
Size: 47 159 24 114 102 14 646 264
Cuts at: 1370 1639 1956
Size: 269 317
Fragments arranged by size:
646 317 269 264 159 114 102 47
24 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1182 1956
Size: 1182 774
BsiHKAI G_wGCw'C
Cuts at: 0 395 1170 1477 1956
Size: 395 775 307 479
Fragments arranged by size:
775 479 395 307
BsmI GAATG_Cn'
Cuts at: 0 1048 1108 1872 1956
Size: 1048 60 764 84
Fragments arranged by size:
1048 764 84 60
Bsp1286I G_dGCh'C
Cuts at: 0 290 395 490 502 586 647 763 1065
Size: 290 105 95 12 84 61 116 302
Cuts at: 1065 1170 1300 1309 1439 1477 1484 1516 1525
Size: 105 130 9 130 38 7 32 9
Cuts at: 1525 1640 1956
Size: 115 316
Fragments arranged by size:
316 302 290 130 130 116 115 105
105 95 84 61 38 32 12 9
9 7
BspEI T'CCGG_A
Cuts at: 0 395 1956
Size: 395 1561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1675 1956
Size: 1675 281
BsrBI CCG'CTC
Cuts at: 0 453 1956
Size: 453 1503
BsrFI r'CCGG_y
Cuts at: 0 563 1956
Size: 563 1393
BssSI C'ACGA_G
Cuts at: 0 402 1956
Size: 402 1554
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1740 1956
Size: 1740 216
BstEII G'GTnAC_C
Cuts at: 0 594 891 1956
Size: 594 297 1065
Fragments arranged by size:
1065 594 297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1628 1956
Size: 12 1616 328
Fragments arranged by size:
1616 328 12
Bsu36I CC'TnA_GG
Cuts at: 0 238 1956
Size: 238 1718
BtgI C'CryG_G
Cuts at: 0 11 963 1403 1700 1766 1916 1956
Size: 11 952 440 297 66 150 40
Fragments arranged by size:
952 440 297 150 66 40 11
BtsI GCAGTG_nn'
Cuts at: 0 153 1221 1956
Size: 153 1068 735
Fragments arranged by size:
1068 735 153
EaeI y'GGCC_r
Cuts at: 0 2 179 659 1250 1703 1772 1956
Size: 2 177 480 591 453 69 184
Fragments arranged by size:
591 480 453 184 177 69 2
EarI CTCTTCn'nnn_
Cuts at: 0 184 1828 1956
Size: 184 1644 128
Fragments arranged by size:
1644 184 128
EcoICRI GAG'CTC
Cuts at: 0 393 1956
Size: 393 1563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 324 352 906 1154 1650 1956
Size: 274 50 28 554 248 496 306
Fragments arranged by size:
554 496 306 274 248 50 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 718 1956
Size: 718 1238
EcoO109I rG'GnC_Cy
Cuts at: 0 486 487 582 729 760 958 1142 1305
Size: 486 1 95 147 31 198 184 163
Cuts at: 1305 1306 1343 1408 1436 1512 1513 1616 1956
Size: 1 37 65 28 76 1 103 340
Fragments arranged by size:
486 340 198 184 163 147 103 95
76 65 37 31 28 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 564 1571 1956
Size: 564 1007 385
Fragments arranged by size:
1007 564 385
HaeII r_GCGC'y
Cuts at: 0 137 420 1956
Size: 137 283 1536
Fragments arranged by size:
1536 283 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 687 719 1867 1899 1956
Size: 687 32 1148 32 57
Fragments arranged by size:
1148 687 57 32 32
HincII GTy'rAC
Cuts at: 0 1077 1956
Size: 1077 879
KpnI G_GTAC'C
Cuts at: 0 1002 1956
Size: 1002 954
MscI TGG'CCA
Cuts at: 0 4 181 1705 1774 1956
Size: 4 177 1524 69 182
Fragments arranged by size:
1524 182 177 69 4
MslI CAynn'nnrTG
Cuts at: 0 10 1768 1956
Size: 10 1758 188
Fragments arranged by size:
1758 188 10
MspA1I CmG'CkG
Cuts at: 0 330 344 986 1005 1020 1097 1660 1956
Size: 330 14 642 19 15 77 563 296
Fragments arranged by size:
642 563 330 296 77 19 15 14
NaeI GCC'GGC
Cuts at: 0 565 1956
Size: 565 1391
NcoI C'CATG_G
Cuts at: 0 11 963 1403 1916 1956
Size: 11 952 440 513 40
Fragments arranged by size:
952 513 440 40 11
NgoMIV G'CCGG_C
Cuts at: 0 563 1956
Size: 563 1393
NspI r_CATG'y
Cuts at: 0 561 1956
Size: 561 1395
PasI CC'CwG_GG
Cuts at: 0 576 1084 1191 1956
Size: 576 508 107 765
Fragments arranged by size:
765 576 508 107
PflMI CCAn_nnn'nTGG
Cuts at: 0 934 1281 1432 1956
Size: 934 347 151 524
Fragments arranged by size:
934 524 347 151
PfoI T'CCnGG_A
Cuts at: 0 1131 1956
Size: 1131 825
PmlI CAC'GTG
Cuts at: 0 947 980 1956
Size: 947 33 976
Fragments arranged by size:
976 947 33
PpuMI rG'GwC_Cy
Cuts at: 0 958 1343 1616 1956
Size: 958 385 273 340
Fragments arranged by size:
958 385 340 273
PshAI GACnn'nnGTC
Cuts at: 0 320 1013 1956
Size: 320 693 943
Fragments arranged by size:
943 693 320
PspOMI G'GGCC_C
Cuts at: 0 486 582 759 1296 1305 1435 1512 1956
Size: 486 96 177 537 9 130 77 444
Fragments arranged by size:
537 486 444 177 130 96 77 9
PspXI vC'TCGA_Gb
Cuts at: 0 1011 1956
Size: 1011 945
PstI C_TGCA'G
Cuts at: 0 1686 1956
Size: 1686 270
PvuII CAG'CTG
Cuts at: 0 344 1005 1020 1660 1956
Size: 344 661 15 640 296
Fragments arranged by size:
661 640 344 296 15
SacI G_AGCT'C
Cuts at: 0 395 1956
Size: 395 1561
SanDI GG'GwC_CC
Cuts at: 0 958 1616 1956
Size: 958 658 340
Fragments arranged by size:
958 658 340
SapI GCTCTTCn'nnn_
Cuts at: 0 184 1956
Size: 184 1772
SbfI CC_TGCA'GG
Cuts at: 0 1686 1956
Size: 1686 270
ScaI AGT'ACT
Cuts at: 0 224 1956
Size: 224 1732
SexAI A'CCwGG_T
Cuts at: 0 887 1956
Size: 887 1069
SfcI C'TryA_G
Cuts at: 0 1682 1956
Size: 1682 274
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1304 1956
Size: 1304 652
SmaI CCC'GGG
Cuts at: 0 54 1301 1956
Size: 54 1247 655
Fragments arranged by size:
1247 655 54
SmlI C'TyrA_G
Cuts at: 0 1011 1528 1594 1956
Size: 1011 517 66 362
Fragments arranged by size:
1011 517 362 66
StyI C'CwwG_G
Cuts at: 0 11 632 963 993 1403 1758 1916 1956
Size: 11 621 331 30 410 355 158 40
Fragments arranged by size:
621 410 355 331 158 40 30 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 1362 1554 1956
Size: 336 1026 192 402
Fragments arranged by size:
1026 402 336 192
TatI w'GTAC_w
Cuts at: 0 222 1928 1956
Size: 222 1706 28
Fragments arranged by size:
1706 222 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 630 951 1956
Size: 630 321 1005
Fragments arranged by size:
1005 630 321
XhoI C'TCGA_G
Cuts at: 0 1011 1956
Size: 1011 945
XmaI C'CCGG_G
Cuts at: 0 52 1299 1956
Size: 52 1247 657
Fragments arranged by size:
1247 657 52
XmnI GAAnn'nnTTC
Cuts at: 0 337 1956
Size: 337 1619
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlwNI ApaI AvaI AvrII BanI BanII BcgI BglI
BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstEII
BstXI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SmaI SmlI StyI TaqII TatI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AlfI AloI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BmtI BplI Bpu10I BsaBI
BsiEI BsiWI BsmBI BspHI BsrDI BsrGI BssHII BstBI
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI MfeI MluI MmeI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PsiI
PsrI PvuI RsrII SacII SalI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI ZraI