Restriction map for
pF1KE0309
(Linear) (Six-base) MAPSORT of: KIEE0309.seq Check: 7646 from: 1 to: 1722
KIEE0309.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 824 886 1722
Size: 824 62 836
Fragments arranged by size:
836 824 62
AccI GT'mk_AC
Cuts at: 0 770 1722
Size: 770 952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1314 1722
Size: 1314 408
AfeI AGC'GCT
Cuts at: 0 1399 1722
Size: 1399 323
AhdI GACnn_n'nnGTC
Cuts at: 0 1602 1722
Size: 1602 120
ApaLI G'TGCA_C
Cuts at: 0 37 1722
Size: 37 1685
ApoI r'AATT_y
Cuts at: 0 142 1722
Size: 142 1580
AvaI C'yCGr_G
Cuts at: 0 566 1722
Size: 566 1156
BanI G'GyrC_C
Cuts at: 0 1370 1511 1571 1722
Size: 1370 141 60 151
Fragments arranged by size:
1370 151 141 60
BanII G_rGCy'C
Cuts at: 0 1252 1722
Size: 1252 470
BbeI G_GCGC'C
Cuts at: 0 1575 1722
Size: 1575 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 1722
Size: 551 1171
BbvCI CC'TCA_GC
Cuts at: 0 1189 1471 1722
Size: 1189 282 251
Fragments arranged by size:
1189 282 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 333 367 837 871 916 950 1722
Size: 333 34 470 34 45 34 772
Fragments arranged by size:
772 470 333 45 34 34 34
BclI T'GATC_A
Cuts at: 0 275 1722
Size: 275 1447
BglI GCCn_nnn'nGGC
Cuts at: 0 268 986 1669 1722
Size: 268 718 683 53
Fragments arranged by size:
718 683 268 53
BglII A'GATC_T
Cuts at: 0 1151 1325 1722
Size: 1151 174 397
Fragments arranged by size:
1151 397 174
BlpI GC'TnA_GC
Cuts at: 0 1048 1722
Size: 1048 674
Bme1580I G_kGCm'C
Cuts at: 0 41 153 264 1722
Size: 41 112 111 1458
Fragments arranged by size:
1458 112 111 41
BmgBI CAC'GTC
Cuts at: 0 117 549 1722
Size: 117 432 1173
Fragments arranged by size:
1173 432 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 696 1722
Size: 696 1026
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1224 1722
Size: 1224 498
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 286 1722
Size: 46 240 1436
Fragments arranged by size:
1436 240 46
Bpu10I CC'TnA_GC
Cuts at: 0 662 1189 1471 1612 1722
Size: 662 527 282 141 110
Fragments arranged by size:
662 527 282 141 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 574 1722
Size: 109 465 1148
Fragments arranged by size:
1148 465 109
BsaI GGTCTCn'nnnn_
Cuts at: 0 1195 1554 1722
Size: 1195 359 168
Fragments arranged by size:
1195 359 168
BsaHI Gr'CG_yC
Cuts at: 0 77 374 1572 1722
Size: 77 297 1198 150
Fragments arranged by size:
1198 297 150 77
BsaWI w'CCGG_w
Cuts at: 0 155 1722
Size: 155 1567
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 100 607 1702 1722
Size: 34 66 507 1095 20
Fragments arranged by size:
1095 507 66 34 20
BseYI C'CCAG_C
Cuts at: 0 944 1267 1722
Size: 944 323 455
Fragments arranged by size:
944 455 323
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 831 1722
Size: 366 465 891
Fragments arranged by size:
891 465 366
BsiEI CG_ry'CG
Cuts at: 0 636 1722
Size: 636 1086
BsiHKAI G_wGCw'C
Cuts at: 0 41 1054 1252 1722
Size: 41 1013 198 470
Fragments arranged by size:
1013 470 198 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1210 1722
Size: 1210 512
Bsp1286I G_dGCh'C
Cuts at: 0 41 153 264 1054 1252 1722
Size: 41 112 111 790 198 470
Fragments arranged by size:
790 470 198 112 111 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 588 665 824 886 1722
Size: 588 77 159 62 836
Fragments arranged by size:
836 588 159 77 62
BsrDI GCAATG_nn'
Cuts at: 0 328 932 1475 1722
Size: 328 604 543 247
Fragments arranged by size:
604 543 328 247
BsrFI r'CCGG_y
Cuts at: 0 806 1403 1722
Size: 806 597 319
Fragments arranged by size:
806 597 319
BsrGI T'GTAC_A
Cuts at: 0 1487 1722
Size: 1487 235
BstAPI GCAn_nnn'nTGC
Cuts at: 0 110 1722
Size: 110 1612
BstEII G'GTnAC_C
Cuts at: 0 1236 1722
Size: 1236 486
BstYI r'GATC_y
Cuts at: 0 30 89 1151 1325 1722
Size: 30 59 1062 174 397
Fragments arranged by size:
1062 397 174 59 30
BtgI C'CryG_G
Cuts at: 0 1124 1196 1667 1722
Size: 1124 72 471 55
Fragments arranged by size:
1124 471 72 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 840 1722
Size: 840 882
BtsI GCAGTG_nn'
Cuts at: 0 1266 1341 1722
Size: 1266 75 381
Fragments arranged by size:
1266 381 75
DraIII CAC_nnn'GTG
Cuts at: 0 501 1722
Size: 501 1221
EaeI y'GGCC_r
Cuts at: 0 835 1035 1308 1559 1722
Size: 835 200 273 251 163
Fragments arranged by size:
835 273 251 200 163
EarI CTCTTCn'nnn_
Cuts at: 0 515 1188 1722
Size: 515 673 534
Fragments arranged by size:
673 534 515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 670 1722
Size: 670 1052
EcoICRI GAG'CTC
Cuts at: 0 1250 1722
Size: 1250 472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 286 1314 1722
Size: 46 240 1028 408
Fragments arranged by size:
1028 408 240 46
EcoO109I rG'GnC_Cy
Cuts at: 0 649 762 882 1722
Size: 649 113 120 840
Fragments arranged by size:
840 649 120 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 568 1722
Size: 568 1154
HaeII r_GCGC'y
Cuts at: 0 807 1401 1575 1722
Size: 807 594 174 147
Fragments arranged by size:
807 594 174 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 655 687 942 974 1165 1197
Size: 547 32 76 32 255 32 191 32
Cuts at: 1197 1239 1271 1588 1620 1722
Size: 42 32 317 32 102
Fragments arranged by size:
547 317 255 191 102 76 42 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1571 1722
Size: 1571 151
MfeI C'AATT_G
Cuts at: 0 939 1722
Size: 939 783
MscI TGG'CCA
Cuts at: 0 1561 1722
Size: 1561 161
MspA1I CmG'CkG
Cuts at: 0 357 1381 1565 1722
Size: 357 1024 184 157
Fragments arranged by size:
1024 357 184 157
NaeI GCC'GGC
Cuts at: 0 808 1405 1722
Size: 808 597 317
Fragments arranged by size:
808 597 317
NarI GG'CG_CC
Cuts at: 0 1572 1722
Size: 1572 150
NcoI C'CATG_G
Cuts at: 0 1124 1667 1722
Size: 1124 543 55
Fragments arranged by size:
1124 543 55
NgoMIV G'CCGG_C
Cuts at: 0 806 1403 1722
Size: 806 597 319
Fragments arranged by size:
806 597 319
NspI r_CATG'y
Cuts at: 0 1389 1722
Size: 1389 333
PasI CC'CwG_GG
Cuts at: 0 1422 1722
Size: 1422 300
PflMI CCAn_nnn'nTGG
Cuts at: 0 944 1722
Size: 944 778
PpuMI rG'GwC_Cy
Cuts at: 0 649 762 1722
Size: 649 113 960
Fragments arranged by size:
960 649 113
PstI C_TGCA'G
Cuts at: 0 356 362 1380 1722
Size: 356 6 1018 342
Fragments arranged by size:
1018 356 342 6
PvuII CAG'CTG
Cuts at: 0 357 1381 1565 1722
Size: 357 1024 184 157
Fragments arranged by size:
1024 357 184 157
SacI G_AGCT'C
Cuts at: 0 1252 1722
Size: 1252 470
SanDI GG'GwC_CC
Cuts at: 0 649 1722
Size: 649 1073
SexAI A'CCwGG_T
Cuts at: 0 734 764 1722
Size: 734 30 958
Fragments arranged by size:
958 734 30
SfcI C'TryA_G
Cuts at: 0 352 358 875 1376 1722
Size: 352 6 517 501 346
Fragments arranged by size:
517 501 352 346 6
SfoI GGC'GCC
Cuts at: 0 1573 1722
Size: 1573 149
SmlI C'TyrA_G
Cuts at: 0 124 553 1722
Size: 124 429 1169
Fragments arranged by size:
1169 429 124
SphI G_CATG'C
Cuts at: 0 1389 1722
Size: 1389 333
StuI AGG'CCT
Cuts at: 0 1063 1722
Size: 1063 659
StyI C'CwwG_G
Cuts at: 0 539 1124 1667 1722
Size: 539 585 543 55
Fragments arranged by size:
585 543 539 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 412 422 1722
Size: 412 10 1300
Fragments arranged by size:
1300 412 10
TatI w'GTAC_w
Cuts at: 0 720 1487 1722
Size: 720 767 235
Fragments arranged by size:
767 720 235
XmnI GAAnn'nnTTC
Cuts at: 0 401 1722
Size: 401 1321
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI
BstEII BstYI BtgI BtgZI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI
MfeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PpuMI PstI PvuII SacI SanDI SexAI
SfcI SfoI SmlI SphI StuI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BfrBI BmtI BsaAI BsaBI BsaXI BsiWI
BsmI BspEI BspHI BsrBI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MluI MmeI MslI NdeI NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI