Restriction map for
pF1KE0311
(Linear) (Six-base) MAPSORT of: KIEE0311.seq Check: 5727 from: 1 to: 3795
KIEE0311.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2307 3795
Size: 2307 1488
AatII G_ACGT'C
Cuts at: 0 1691 3229 3795
Size: 1691 1538 566
Fragments arranged by size:
1691 1538 566
AccI GT'mk_AC
Cuts at: 0 3319 3795
Size: 3319 476
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 2192 3701 3795
Size: 554 1638 1509 94
Fragments arranged by size:
1638 1509 554 94
AflIII A'CryG_T
Cuts at: 0 1883 2793 3795
Size: 1883 910 1002
Fragments arranged by size:
1883 1002 910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 469 3795
Size: 469 3326
AlwNI CAG_nnn'CTG
Cuts at: 0 3094 3795
Size: 3094 701
ApoI r'AATT_y
Cuts at: 0 71 210 267 1534 1552 1569 1699 1737
Size: 71 139 57 1267 18 17 130 38
Cuts at: 1737 2581 2619 2924 3522 3592 3795
Size: 844 38 305 598 70 203
Fragments arranged by size:
1267 844 598 305 203 139 130 71
70 57 38 38 18 17
AseI AT'TA_AT
Cuts at: 0 1826 3795
Size: 1826 1969
BbsI GAAGACnn'nnnn_
Cuts at: 0 451 1861 2161 2282 2743 3253 3795
Size: 451 1410 300 121 461 510 542
Fragments arranged by size:
1410 542 510 461 451 300 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1107 1141 3795
Size: 1107 34 2654
Fragments arranged by size:
2654 1107 34
BclI T'GATC_A
Cuts at: 0 2919 3795
Size: 2919 876
BglII A'GATC_T
Cuts at: 0 1303 2817 3162 3409 3708 3795
Size: 1303 1514 345 247 299 87
Fragments arranged by size:
1514 1303 345 299 247 87
BlpI GC'TnA_GC
Cuts at: 0 2687 3795
Size: 2687 1108
Bme1580I G_kGCm'C
Cuts at: 0 10 3795
Size: 10 3785
BmrI ACTGGGnnnn_n'
Cuts at: 0 4 759 3103 3795
Size: 4 755 2344 692
Fragments arranged by size:
2344 755 692 4
BmtI G_CTAG'C
Cuts at: 0 419 3795
Size: 419 3376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3251 3795
Size: 3251 544
Bpu10I CC'TnA_GC
Cuts at: 0 185 1394 2134 2716 3795
Size: 185 1209 740 582 1079
Fragments arranged by size:
1209 1079 740 582 185
BsaBI GATnn'nnATC
Cuts at: 0 3095 3795
Size: 3095 700
BsaHI Gr'CG_yC
Cuts at: 0 1688 3226 3795
Size: 1688 1538 569
Fragments arranged by size:
1688 1538 569
BsaWI w'CCGG_w
Cuts at: 0 1850 3605 3795
Size: 1850 1755 190
Fragments arranged by size:
1850 1755 190
BseYI C'CCAG_C
Cuts at: 0 2007 3543 3795
Size: 2007 1536 252
Fragments arranged by size:
2007 1536 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 3795
Size: 1352 2443
BsiHKAI G_wGCw'C
Cuts at: 0 3541 3795
Size: 3541 254
BsmI GAATG_Cn'
Cuts at: 0 2863 3795
Size: 2863 932
Bsp1286I G_dGCh'C
Cuts at: 0 10 3541 3795
Size: 10 3531 254
Fragments arranged by size:
3531 254 10
BspHI T'CATG_A
Cuts at: 0 338 3795
Size: 338 3457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2307 2394 3795
Size: 2307 87 1401
Fragments arranged by size:
2307 1401 87
BsrGI T'GTAC_A
Cuts at: 0 2496 3795
Size: 2496 1299
BstXI CCAn_nnnn'nTGG
Cuts at: 0 772 3795
Size: 772 3023
BstYI r'GATC_y
Cuts at: 0 981 1303 1338 2817 3162 3258 3409 3708
Size: 981 322 35 1479 345 96 151 299
Cuts at: 3708 3795
Size: 87
Fragments arranged by size:
1479 981 345 322 299 151 96 87
35
Bsu36I CC'TnA_GG
Cuts at: 0 39 3795
Size: 39 3756
BtsI GCAGTG_nn'
Cuts at: 0 529 3680 3795
Size: 529 3151 115
Fragments arranged by size:
3151 529 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2301 2336 3795
Size: 2301 35 1459
Fragments arranged by size:
2301 1459 35
DraI TTT'AAA
Cuts at: 0 161 1251 2829 3068 3597 3762 3795
Size: 161 1090 1578 239 529 165 33
Fragments arranged by size:
1578 1090 529 239 165 161 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 3231 3795
Size: 3231 564
EaeI y'GGCC_r
Cuts at: 0 774 2370 3795
Size: 774 1596 1425
Fragments arranged by size:
1596 1425 774
EarI CTCTTCn'nnn_
Cuts at: 0 573 3795
Size: 573 3222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 2192 3251 3701 3795
Size: 554 1638 1059 450 94
Fragments arranged by size:
1638 1059 554 450 94
EcoO109I rG'GnC_Cy
Cuts at: 0 1754 2051 3795
Size: 1754 297 1744
Fragments arranged by size:
1754 1744 297
EcoRI G'AATT_C
Cuts at: 0 210 1569 3795
Size: 210 1359 2226
Fragments arranged by size:
2226 1359 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1613 3405 3795
Size: 1613 1792 390
Fragments arranged by size:
1792 1613 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 778 810 921 953 1198 1230
Size: 550 32 196 32 111 32 245 32
Cuts at: 1230 1295 1327 2665 2697 3795
Size: 65 32 1338 32 1098
Fragments arranged by size:
1338 1098 550 245 196 111 65 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2895 3026 3603 3795
Size: 2895 131 577 192
Fragments arranged by size:
2895 577 192 131
HindIII A'AGCT_T
Cuts at: 0 316 1595 1831 2131 3795
Size: 316 1279 236 300 1664
Fragments arranged by size:
1664 1279 316 300 236
HpaI GTT'AAC
Cuts at: 0 3026 3795
Size: 3026 769
MfeI C'AATT_G
Cuts at: 0 1063 3795
Size: 1063 2732
MscI TGG'CCA
Cuts at: 0 2372 3795
Size: 2372 1423
MslI CAynn'nnrTG
Cuts at: 0 944 1886 2089 2372 3795
Size: 944 942 203 283 1423
Fragments arranged by size:
1423 944 942 283 203
MspA1I CmG'CkG
Cuts at: 0 1368 1389 3795
Size: 1368 21 2406
Fragments arranged by size:
2406 1368 21
NheI G'CTAG_C
Cuts at: 0 415 3795
Size: 415 3380
NspI r_CATG'y
Cuts at: 0 1887 2797 3795
Size: 1887 910 998
Fragments arranged by size:
1887 998 910
PciI A'CATG_T
Cuts at: 0 1883 2793 3795
Size: 1883 910 1002
Fragments arranged by size:
1883 1002 910
PfoI T'CCnGG_A
Cuts at: 0 1313 3099 3795
Size: 1313 1786 696
Fragments arranged by size:
1786 1313 696
PpuMI rG'GwC_Cy
Cuts at: 0 1754 2051 3795
Size: 1754 297 1744
Fragments arranged by size:
1754 1744 297
PstI C_TGCA'G
Cuts at: 0 861 3795
Size: 861 2934
PvuII CAG'CTG
Cuts at: 0 1368 1389 3795
Size: 1368 21 2406
Fragments arranged by size:
2406 1368 21
SanDI GG'GwC_CC
Cuts at: 0 1754 3795
Size: 1754 2041
SfcI C'TryA_G
Cuts at: 0 680 857 2046 3795
Size: 680 177 1189 1749
Fragments arranged by size:
1749 1189 680 177
SpeI A'CTAG_T
Cuts at: 0 3079 3795
Size: 3079 716
SspI AAT'ATT
Cuts at: 0 537 3795
Size: 537 3258
StyI C'CwwG_G
Cuts at: 0 3530 3795
Size: 3530 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2653 3795
Size: 2653 1142
TatI w'GTAC_w
Cuts at: 0 1458 2496 3745 3795
Size: 1458 1038 1249 50
Fragments arranged by size:
1458 1249 1038 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 810 842 3795
Size: 810 32 2953
Fragments arranged by size:
2953 810 32
XbaI T'CTAG_A
Cuts at: 0 1600 3795
Size: 1600 2195
XmnI GAAnn'nnTTC
Cuts at: 0 1189 2453 3795
Size: 1189 1264 1342
Fragments arranged by size:
1342 1264 1189
ZraI GAC'GTC
Cuts at: 0 1689 3227 3795
Size: 1689 1538 568
Fragments arranged by size:
1689 1538 568
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AlwNI ApoI
AseI BbsI BcgI BclI BglII BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BsaBI BsaHI BsaWI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstXI BstYI
Bsu36I BtsI CspCI DraI DrdI EaeI EarI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI MfeI
MscI MslI MspA1I NheI NspI PciI PfoI PpuMI
PstI PvuII SanDI SfcI SpeI SspI StyI TaqII
TatI TstI XbaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbvCI BciVI BfrBI BglI BmgBI
BplI BpuEI BsaI BsaAI BsaXI BseRI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgI BtgZI ClaI DraIII EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
KasI KpnI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI
SrfI StuI SwaI Tth111I XcmI XhoI XmaI