Restriction map for
pF1KE0312
(Linear) (Six-base) MAPSORT of: KIEE0312.seq Check: 6782 from: 1 to: 1575
KIEE0312.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 310 1283 1575
Size: 310 973 292
Fragments arranged by size:
973 310 292
AccI GT'mk_AC
Cuts at: 0 754 1575
Size: 754 821
Acc65I G'GTAC_C
Cuts at: 0 1361 1575
Size: 1361 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1417 1535 1575
Size: 553 864 118 40
Fragments arranged by size:
864 553 118 40
AfeI AGC'GCT
Cuts at: 0 914 1575
Size: 914 661
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1148 1180 1575
Size: 1148 32 395
Fragments arranged by size:
1148 395 32
AlwNI CAG_nnn'CTG
Cuts at: 0 217 730 815 1284 1575
Size: 217 513 85 469 291
Fragments arranged by size:
513 469 291 217 85
ApaI G_GGCC'C
Cuts at: 0 168 1575
Size: 168 1407
AvaI C'yCGr_G
Cuts at: 0 272 363 1130 1575
Size: 272 91 767 445
Fragments arranged by size:
767 445 272 91
BanI G'GyrC_C
Cuts at: 0 1210 1305 1361 1575
Size: 1210 95 56 214
Fragments arranged by size:
1210 214 95 56
BanII G_rGCy'C
Cuts at: 0 168 460 980 1427 1575
Size: 168 292 520 447 148
Fragments arranged by size:
520 447 292 168 148
BbvCI CC'TCA_GC
Cuts at: 0 218 1271 1575
Size: 218 1053 304
Fragments arranged by size:
1053 304 218
BciVI GTATCCnnnnn_n'
Cuts at: 0 67 1575
Size: 67 1508
BglI GCCn_nnn'nGGC
Cuts at: 0 1208 1389 1575
Size: 1208 181 186
Fragments arranged by size:
1208 186 181
Bme1580I G_kGCm'C
Cuts at: 0 168 1213 1575
Size: 168 1045 362
Fragments arranged by size:
1045 362 168
BmgBI CAC'GTC
Cuts at: 0 927 1575
Size: 927 648
BmrI ACTGGGnnnn_n'
Cuts at: 0 1020 1086 1575
Size: 1020 66 489
Fragments arranged by size:
1020 489 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 882 1575
Size: 882 693
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1575
Size: 646 929
Bpu10I CC'TnA_GC
Cuts at: 0 158 218 1271 1299 1575
Size: 158 60 1053 28 276
Fragments arranged by size:
1053 276 158 60 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 964 1575
Size: 320 644 611
Fragments arranged by size:
644 611 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1148 1178 1575
Size: 1148 30 397
Fragments arranged by size:
1148 397 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 40 979 982 1575
Size: 37 3 939 3 593
Fragments arranged by size:
939 593 37 3 3
BseYI C'CCAG_C
Cuts at: 0 210 650 1265 1575
Size: 210 440 615 310
Fragments arranged by size:
615 440 310 210
BsiHKAI G_wGCw'C
Cuts at: 0 980 1300 1575
Size: 980 320 275
Fragments arranged by size:
980 320 275
Bsp1286I G_dGCh'C
Cuts at: 0 168 460 980 1213 1300 1427 1575
Size: 168 292 520 233 87 127 148
Fragments arranged by size:
520 292 233 168 148 127 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 310 444 1283 1575
Size: 310 134 839 292
Fragments arranged by size:
839 310 292 134
BsrBI CCG'CTC
Cuts at: 0 53 162 181 942 1257 1575
Size: 53 109 19 761 315 318
Fragments arranged by size:
761 318 315 109 53 19
BsrDI GCAATG_nn'
Cuts at: 0 705 1564 1575
Size: 705 859 11
Fragments arranged by size:
859 705 11
BsrFI r'CCGG_y
Cuts at: 0 146 371 1575
Size: 146 225 1204
Fragments arranged by size:
1204 225 146
BstAPI GCAn_nnn'nTGC
Cuts at: 0 305 1575
Size: 305 1270
BstZ17I GTA'TAC
Cuts at: 0 755 1575
Size: 755 820
Bsu36I CC'TnA_GG
Cuts at: 0 827 1310 1575
Size: 827 483 265
Fragments arranged by size:
827 483 265
BtgI C'CryG_G
Cuts at: 0 1365 1575
Size: 1365 210
BtsI GCAGTG_nn'
Cuts at: 0 1474 1575
Size: 1474 101
EaeI y'GGCC_r
Cuts at: 0 245 1329 1575
Size: 245 1084 246
Fragments arranged by size:
1084 246 245
EarI CTCTTCn'nnn_
Cuts at: 0 107 737 842 851 1393 1575
Size: 107 630 105 9 542 182
Fragments arranged by size:
630 542 182 107 105 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 1257 1471 1575
Size: 34 1223 214 104
Fragments arranged by size:
1223 214 104 34
EcoICRI GAG'CTC
Cuts at: 0 978 1575
Size: 978 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 646 1417 1535 1575
Size: 553 93 771 118 40
Fragments arranged by size:
771 553 118 93 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1530 1575
Size: 1530 45
EcoO109I rG'GnC_Cy
Cuts at: 0 165 1281 1314 1403 1575
Size: 165 1116 33 89 172
Fragments arranged by size:
1116 172 165 89 33
HaeII r_GCGC'y
Cuts at: 0 7 693 916 1224 1575
Size: 7 686 223 308 351
Fragments arranged by size:
686 351 308 223 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1546 1575
Size: 1124 32 390 29
Fragments arranged by size:
1124 390 32 29
KpnI G_GTAC'C
Cuts at: 0 1365 1575
Size: 1365 210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1575
Size: 475 1100
MscI TGG'CCA
Cuts at: 0 1331 1575
Size: 1331 244
MslI CAynn'nnrTG
Cuts at: 0 869 1093 1501 1575
Size: 869 224 408 74
Fragments arranged by size:
869 408 224 74
MspA1I CmG'CkG
Cuts at: 0 217 654 1575
Size: 217 437 921
Fragments arranged by size:
921 437 217
NcoI C'CATG_G
Cuts at: 0 1365 1575
Size: 1365 210
NspI r_CATG'y
Cuts at: 0 360 1575
Size: 360 1215
PflMI CCAn_nnn'nTGG
Cuts at: 0 1284 1575
Size: 1284 291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1148 1180 1575
Size: 1148 32 395
Fragments arranged by size:
1148 395 32
PspOMI G'GGCC_C
Cuts at: 0 164 1575
Size: 164 1411
PstI C_TGCA'G
Cuts at: 0 491 659 1294 1575
Size: 491 168 635 281
Fragments arranged by size:
635 491 281 168
PvuII CAG'CTG
Cuts at: 0 654 1575
Size: 654 921
SacI G_AGCT'C
Cuts at: 0 980 1575
Size: 980 595
SapI GCTCTTCn'nnn_
Cuts at: 0 107 737 1575
Size: 107 630 838
Fragments arranged by size:
838 630 107
SexAI A'CCwGG_T
Cuts at: 0 41 1575
Size: 41 1534
SfcI C'TryA_G
Cuts at: 0 487 655 1290 1378 1575
Size: 487 168 635 88 197
Fragments arranged by size:
635 487 197 168 88
SgrAI Cr'CCGG_yG
Cuts at: 0 371 1575
Size: 371 1204
SmlI C'TyrA_G
Cuts at: 0 272 335 979 1575
Size: 272 63 644 596
Fragments arranged by size:
644 596 272 63
StyI C'CwwG_G
Cuts at: 0 780 1229 1365 1575
Size: 780 449 136 210
Fragments arranged by size:
780 449 210 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 664 1509 1575
Size: 664 845 66
Fragments arranged by size:
845 664 66
TatI w'GTAC_w
Cuts at: 0 359 1097 1575
Size: 359 738 478
Fragments arranged by size:
738 478 359
Tth111I GACn'n_nGTC
Cuts at: 0 1138 1470 1575
Size: 1138 332 105
Fragments arranged by size:
1138 332 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 579 1575
Size: 579 996
XhoI C'TCGA_G
Cuts at: 0 272 1575
Size: 272 1303
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AloI AlwNI ApaI
AvaI BanI BanII BbvCI BciVI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstZ17I
Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KpnI MmeI MscI MslI
MspA1I NcoI NspI PflMI PpiI PspOMI PstI PvuII
SacI SapI SexAI SfcI SgrAI SmlI StyI TaqII
TatI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BcgI BclI BfrBI BglII BlpI BmtI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XmaI XmnI ZraI