Restriction map for
pF1KE0313
(Linear) (Six-base) MAPSORT of: KIEE0313.seq Check: 9058 from: 1 to: 3009
KIEE0313.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1608 3009
Size: 1608 1401
AatII G_ACGT'C
Cuts at: 0 1442 3009
Size: 1442 1567
AccI GT'mk_AC
Cuts at: 0 2830 3009
Size: 2830 179
Acc65I G'GTAC_C
Cuts at: 0 1761 3009
Size: 1761 1248
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 769 907 1012 2411 2440 3009
Size: 213 556 138 105 1399 29 569
Fragments arranged by size:
1399 569 556 213 138 105 29
AflII C'TTAA_G
Cuts at: 0 2695 3009
Size: 2695 314
AhdI GACnn_n'nnGTC
Cuts at: 0 1080 3009
Size: 1080 1929
AleI CACnn'nnGTG
Cuts at: 0 1600 3009
Size: 1600 1409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 463 2276 3009
Size: 463 1813 733
Fragments arranged by size:
1813 733 463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 581 613 3009
Size: 581 32 2396
Fragments arranged by size:
2396 581 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1359 1678 3009
Size: 1359 319 1331
Fragments arranged by size:
1359 1331 319
ApaI G_GGCC'C
Cuts at: 0 392 574 2149 2924 3009
Size: 392 182 1575 775 85
Fragments arranged by size:
1575 775 392 182 85
ApaLI G'TGCA_C
Cuts at: 0 1249 3009
Size: 1249 1760
ApoI r'AATT_y
Cuts at: 0 2599 2877 3009
Size: 2599 278 132
Fragments arranged by size:
2599 278 132
AvaI C'yCGr_G
Cuts at: 0 215 401 1433 2729 3009
Size: 215 186 1032 1296 280
Fragments arranged by size:
1296 1032 280 215 186
BanI G'GyrC_C
Cuts at: 0 1086 1428 1537 1579 1761 3009
Size: 1086 342 109 42 182 1248
Fragments arranged by size:
1248 1086 342 182 109 42
BanII G_rGCy'C
Cuts at: 0 392 574 2149 2470 2924 3008 3009
Size: 392 182 1575 321 454 84 1
Fragments arranged by size:
1575 454 392 321 182 84 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 3009
Size: 551 2458
BciVI GTATCCnnnnn_n'
Cuts at: 0 2089 3009
Size: 2089 920
BclI T'GATC_A
Cuts at: 0 1908 3009
Size: 1908 1101
BglI GCCn_nnn'nGGC
Cuts at: 0 2153 3009
Size: 2153 856
BglII A'GATC_T
Cuts at: 0 326 3009
Size: 326 2683
BlpI GC'TnA_GC
Cuts at: 0 1279 2902 3009
Size: 1279 1623 107
Fragments arranged by size:
1623 1279 107
Bme1580I G_kGCm'C
Cuts at: 0 392 574 1091 1253 1380 1540 2149 2924
Size: 392 182 517 162 127 160 609 775
Cuts at: 2924 3009
Size: 85
Fragments arranged by size:
775 609 517 392 182 162 160 127
85
BmgBI CAC'GTC
Cuts at: 0 252 3009
Size: 252 2757
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 519 577 1800 1890 2101 2160 3009
Size: 474 45 58 1223 90 211 59 849
Fragments arranged by size:
1223 849 474 211 90 59 58 45
BmtI G_CTAG'C
Cuts at: 0 322 3009
Size: 322 2687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1000 1057 1099 1156 1166 1402 1510
Size: 874 126 57 42 57 10 236 108
Cuts at: 1510 1559 2128 2824 3009
Size: 49 569 696 185
Fragments arranged by size:
874 696 569 236 185 126 108 57
57 49 42 10
Bpu10I CC'TnA_GC
Cuts at: 0 839 1300 2464 3009
Size: 839 461 1164 545
Fragments arranged by size:
1164 839 545 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1453 3009
Size: 1453 1556
BsaI GGTCTCn'nnnn_
Cuts at: 0 819 1893 2030 2204 3009
Size: 819 1074 137 174 805
Fragments arranged by size:
1074 819 805 174 137
BsaHI Gr'CG_yC
Cuts at: 0 1439 2483 3009
Size: 1439 1044 526
Fragments arranged by size:
1439 1044 526
BsaWI w'CCGG_w
Cuts at: 0 784 1481 2498 3009
Size: 784 697 1017 511
Fragments arranged by size:
1017 784 697 511
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2977 3007 3009
Size: 2977 30 2
Fragments arranged by size:
2977 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 153 285 3009
Size: 153 132 2724
Fragments arranged by size:
2724 153 132
BseYI C'CCAG_C
Cuts at: 0 242 282 630 654 870 974 1741 2049
Size: 242 40 348 24 216 104 767 308
Cuts at: 2049 2294 3009
Size: 245 715
Fragments arranged by size:
767 715 348 308 245 242 216 104
40 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 2683 3009
Size: 1015 1668 326
Fragments arranged by size:
1668 1015 326
BsiEI CG_ry'CG
Cuts at: 0 240 1482 3009
Size: 240 1242 1527
Fragments arranged by size:
1527 1242 240
BsiHKAI G_wGCw'C
Cuts at: 0 644 1253 3008 3009
Size: 644 609 1755 1
Fragments arranged by size:
1755 644 609 1
BsmI GAATG_Cn'
Cuts at: 0 586 3009
Size: 586 2423
Bsp1286I G_dGCh'C
Cuts at: 0 392 574 644 1091 1253 1380 1540 2149
Size: 392 182 70 447 162 127 160 609
Cuts at: 2149 2470 2924 3008 3009
Size: 321 454 84 1
Fragments arranged by size:
609 454 447 392 321 182 162 160
127 84 70 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1608 1871 2870 3009
Size: 1608 263 999 139
Fragments arranged by size:
1608 999 263 139
BsrBI CCG'CTC
Cuts at: 0 99 2112 2952 3009
Size: 99 2013 840 57
Fragments arranged by size:
2013 840 99 57
BsrDI GCAATG_nn'
Cuts at: 0 698 3009
Size: 698 2311
BsrFI r'CCGG_y
Cuts at: 0 20 685 1582 1876 3009
Size: 20 665 897 294 1133
Fragments arranged by size:
1133 897 665 294 20
BsrGI T'GTAC_A
Cuts at: 0 2135 3009
Size: 2135 874
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2025 3009
Size: 2025 984
BstEII G'GTnAC_C
Cuts at: 0 798 3009
Size: 798 2211
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 2086 2214 3009
Size: 618 1468 128 795
Fragments arranged by size:
1468 795 618 128
BstYI r'GATC_y
Cuts at: 0 326 2670 3009
Size: 326 2344 339
Fragments arranged by size:
2344 339 326
BstZ17I GTA'TAC
Cuts at: 0 2831 3009
Size: 2831 178
Bsu36I CC'TnA_GG
Cuts at: 0 457 898 950 1766 2916 3009
Size: 457 441 52 816 1150 93
Fragments arranged by size:
1150 816 457 441 93 52
BtsI GCAGTG_nn'
Cuts at: 0 313 941 1871 1944 2101 3009
Size: 313 628 930 73 157 908
Fragments arranged by size:
930 908 628 313 157 73
ClaI AT'CG_AT
Cuts at: 0 1931 3009
Size: 1931 1078
DraIII CAC_nnn'GTG
Cuts at: 0 1257 3009
Size: 1257 1752
EaeI y'GGCC_r
Cuts at: 0 1941 3009
Size: 1941 1068
EarI CTCTTCn'nnn_
Cuts at: 0 508 2387 3009
Size: 508 1879 622
Fragments arranged by size:
1879 622 508
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 412 488 3009
Size: 412 76 2521
Fragments arranged by size:
2521 412 76
EcoICRI GAG'CTC
Cuts at: 0 3006 3009
Size: 3006 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 769 874 907 1000 1012 1057 1099
Size: 213 556 105 33 93 12 45 42
Cuts at: 1099 1156 1166 1402 1510 1559 2128 2411 2440
Size: 57 10 236 108 49 569 283 29
Cuts at: 2440 2824 3009
Size: 384 185
Fragments arranged by size:
569 556 384 283 236 213 185 108
105 93 57 49 45 42 33 29
12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2866 2944 3009
Size: 2866 78 65
Fragments arranged by size:
2866 78 65
EcoO109I rG'GnC_Cy
Cuts at: 0 143 388 389 571 658 1101 1224 2053
Size: 143 245 1 182 87 443 123 829
Cuts at: 2053 2145 2146 2739 2920 2921 2967 3009
Size: 92 1 593 181 1 46 42
Fragments arranged by size:
829 593 443 245 182 181 143 123
92 87 46 42 1 1 1
EcoRI G'AATT_C
Cuts at: 0 2599 3009
Size: 2599 410
EcoRV GAT'ATC
Cuts at: 0 1062 3009
Size: 1062 1947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 153 630 1299 1353 3009
Size: 153 477 669 54 1656
Fragments arranged by size:
1656 669 477 153 54
HaeII r_GCGC'y
Cuts at: 0 871 1398 3009
Size: 871 527 1611
Fragments arranged by size:
1611 871 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 1066 1098 3009
Size: 177 32 857 32 1911
Fragments arranged by size:
1911 857 177 32 32
KpnI G_GTAC'C
Cuts at: 0 1765 3009
Size: 1765 1244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1691 3009
Size: 1691 1318
MscI TGG'CCA
Cuts at: 0 1943 3009
Size: 1943 1066
MslI CAynn'nnrTG
Cuts at: 0 1600 1756 2752 2930 3009
Size: 1600 156 996 178 79
Fragments arranged by size:
1600 996 178 156 79
MspA1I CmG'CkG
Cuts at: 0 17 242 747 906 978 1359 1446 1509
Size: 17 225 505 159 72 381 87 63
Cuts at: 1509 2022 2049 2294 2667 3009
Size: 513 27 245 373 342
Fragments arranged by size:
513 505 381 373 342 245 225 159
87 72 63 27 17
NaeI GCC'GGC
Cuts at: 0 22 687 1878 3009
Size: 22 665 1191 1131
Fragments arranged by size:
1191 1131 665 22
NgoMIV G'CCGG_C
Cuts at: 0 20 685 1876 3009
Size: 20 665 1191 1133
Fragments arranged by size:
1191 1133 665 20
NheI G'CTAG_C
Cuts at: 0 318 3009
Size: 318 2691
NruI TCG'CGA
Cuts at: 0 95 3009
Size: 95 2914
PflMI CCAn_nnn'nTGG
Cuts at: 0 1097 1650 1950 3009
Size: 1097 553 300 1059
Fragments arranged by size:
1097 1059 553 300
PfoI T'CCnGG_A
Cuts at: 0 29 1143 2010 2213 2801 3009
Size: 29 1114 867 203 588 208
Fragments arranged by size:
1114 867 588 208 203 29
PpuMI rG'GwC_Cy
Cuts at: 0 143 658 1101 1224 3009
Size: 143 515 443 123 1785
Fragments arranged by size:
1785 515 443 143 123
PshAI GACnn'nnGTC
Cuts at: 0 217 1484 3009
Size: 217 1267 1525
Fragments arranged by size:
1525 1267 217
PsiI TTA'TAA
Cuts at: 0 116 3009
Size: 116 2893
PspOMI G'GGCC_C
Cuts at: 0 388 570 2145 2920 3009
Size: 388 182 1575 775 89
Fragments arranged by size:
1575 775 388 182 89
PstI C_TGCA'G
Cuts at: 0 1049 1514 1841 2255 3009
Size: 1049 465 327 414 754
Fragments arranged by size:
1049 754 465 414 327
PvuII CAG'CTG
Cuts at: 0 17 747 906 978 1509 2022 2049 2667
Size: 17 730 159 72 531 513 27 618
Cuts at: 2667 3009
Size: 342
Fragments arranged by size:
730 618 531 513 342 159 72 27
17
SacI G_AGCT'C
Cuts at: 0 3008 3009
Size: 3008 1
SanDI GG'GwC_CC
Cuts at: 0 143 1101 3009
Size: 143 958 1908
Fragments arranged by size:
1908 958 143
SfcI C'TryA_G
Cuts at: 0 1045 1510 1837 1973 2193 2251 3009
Size: 1045 465 327 136 220 58 758
Fragments arranged by size:
1045 758 465 327 220 136 58
SmaI CCC'GGG
Cuts at: 0 2731 3009
Size: 2731 278
SmlI C'TyrA_G
Cuts at: 0 1468 2695 3009
Size: 1468 1227 314
Fragments arranged by size:
1468 1227 314
StuI AGG'CCT
Cuts at: 0 2257 3009
Size: 2257 752
StyI C'CwwG_G
Cuts at: 0 2604 2735 3009
Size: 2604 131 274
Fragments arranged by size:
2604 274 131
TatI w'GTAC_w
Cuts at: 0 2135 3009
Size: 2135 874
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 351 383 779 811 1530 1562 2977 3009
Size: 351 32 396 32 719 32 1415 32
Fragments arranged by size:
1415 719 396 351 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1270 3009
Size: 1270 1739
XmaI C'CCGG_G
Cuts at: 0 2729 3009
Size: 2729 280
XmnI GAAnn'nnTTC
Cuts at: 0 2797 3009
Size: 2797 212
ZraI GAC'GTC
Cuts at: 0 1440 3009
Size: 1440 1569
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtsI ClaI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I
NaeI NgoMIV NheI NruI PflMI PfoI PpuMI PshAI
PsiI PspOMI PstI PvuII SacI SanDI SfcI SmaI
SmlI StuI StyI TatI TstI Tth111I XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AfeI AflIII AgeI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BfrBI BplI BsaAI
BsaBI BsiWI BsmBI BspEI BspHI BssHII BssSI BstBI
BtgI BtgZI CspCI DraI DrdI EagI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI NarI
NcoI NdeI NotI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
XbaI XcmI XhoI