(Linear) (Six-base) MAPSORT of: KIEE0314.seq Check: 4981 from: 1 to: 2148 KIEE0314.orf With 182 enzymes: SgfI * April 24, 2009 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1495 2148 Size: 1495 653 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 542 949 2012 2148 Size: 542 407 1063 136 Fragments arranged by size: 1063 542 407 136 AfeI AGC'GCT Cuts at: 0 803 2148 Size: 803 1345 AflII C'TTAA_G Cuts at: 0 1036 2148 Size: 1036 1112 AflIII A'CryG_T Cuts at: 0 1456 2148 Size: 1456 692 AlwNI CAG_nnn'CTG Cuts at: 0 481 862 2148 Size: 481 381 1286 Fragments arranged by size: 1286 481 381 ApoI r'AATT_y Cuts at: 0 841 976 1412 1443 2148 Size: 841 135 436 31 705 Fragments arranged by size: 841 705 436 135 31 AseI AT'TA_AT Cuts at: 0 460 2148 Size: 460 1688 AvaI C'yCGr_G Cuts at: 0 1010 1682 2148 Size: 1010 672 466 Fragments arranged by size: 1010 672 466 AvrII C'CTAG_G Cuts at: 0 556 2148 Size: 556 1592 BamHI G'GATC_C Cuts at: 0 822 1628 2148 Size: 822 806 520 Fragments arranged by size: 822 806 520 BanI G'GyrC_C Cuts at: 0 696 1274 2148 Size: 696 578 874 Fragments arranged by size: 874 696 578 BanII G_rGCy'C Cuts at: 0 593 1133 1164 2148 Size: 593 540 31 984 Fragments arranged by size: 984 593 540 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 404 488 2000 2148 Size: 404 84 1512 148 Fragments arranged by size: 1512 404 148 84 BciVI GTATCCnnnnn_n' Cuts at: 0 563 2148 Size: 563 1585 BfrBI ATG'CAT Cuts at: 0 1381 1393 2148 Size: 1381 12 755 Fragments arranged by size: 1381 755 12 BglI GCCn_nnn'nGGC Cuts at: 0 118 2148 Size: 118 2030 BglII A'GATC_T Cuts at: 0 421 2148 Size: 421 1727 Bme1580I G_kGCm'C Cuts at: 0 701 1279 1547 2148 Size: 701 578 268 601 Fragments arranged by size: 701 601 578 268 BmrI ACTGGGnnnn_n' Cuts at: 0 78 2148 Size: 78 2070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 941 2148 Size: 941 1207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 28 89 2148 Size: 21 7 61 2059 Fragments arranged by size: 2059 61 21 7 Bpu10I CC'TnA_GC Cuts at: 0 584 1688 2148 Size: 584 1104 460 Fragments arranged by size: 1104 584 460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1612 1975 2148 Size: 1612 363 173 Fragments arranged by size: 1612 363 173 BsaI GGTCTCn'nnnn_ Cuts at: 0 929 2148 Size: 929 1219 BsaBI GATnn'nnATC Cuts at: 0 426 2148 Size: 426 1722 BsaWI w'CCGG_w Cuts at: 0 714 2148 Size: 714 1434 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 62 92 2148 Size: 62 30 2056 Fragments arranged by size: 2056 62 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 163 166 292 507 541 772 1178 2148 Size: 163 3 126 215 34 231 406 970 Fragments arranged by size: 970 406 231 215 163 126 34 3 BseYI C'CCAG_C Cuts at: 0 172 2148 Size: 172 1976 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1523 2148 Size: 1523 625 BsiHKAI G_wGCw'C Cuts at: 0 593 1133 2148 Size: 593 540 1015 Fragments arranged by size: 1015 593 540 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1969 2148 Size: 1969 179 Bsp1286I G_dGCh'C Cuts at: 0 593 701 1133 1164 1279 1547 2148 Size: 593 108 432 31 115 268 601 Fragments arranged by size: 601 593 432 268 115 108 31 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1495 2148 Size: 1495 653 BstYI r'GATC_y Cuts at: 0 421 822 1628 2148 Size: 421 401 806 520 Fragments arranged by size: 806 520 421 401 Bsu36I CC'TnA_GG Cuts at: 0 1144 2148 Size: 1144 1004 BtgI C'CryG_G Cuts at: 0 1547 2148 Size: 1547 601 BtsI GCAGTG_nn' Cuts at: 0 1825 2148 Size: 1825 323 ClaI AT'CG_AT Cuts at: 0 1059 2148 Size: 1059 1089 DraI TTT'AAA Cuts at: 0 368 975 1065 1448 2148 Size: 368 607 90 383 700 Fragments arranged by size: 700 607 383 368 90 DraIII CAC_nnn'GTG Cuts at: 0 18 2148 Size: 18 2130 EaeI y'GGCC_r Cuts at: 0 2 2148 Size: 2 2146 EarI CTCTTCn'nnn_ Cuts at: 0 140 293 420 518 812 1121 1610 2117 Size: 140 153 127 98 294 309 489 507 Cuts at: 2117 2148 Size: 31 Fragments arranged by size: 507 489 309 294 153 140 127 98 31 EcoICRI GAG'CTC Cuts at: 0 591 1131 2148 Size: 591 540 1017 Fragments arranged by size: 1017 591 540 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 28 89 542 949 2012 2148 Size: 21 7 61 453 407 1063 136 Fragments arranged by size: 1063 453 407 136 61 21 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 485 2148 Size: 485 1663 EcoO109I rG'GnC_Cy Cuts at: 0 99 111 1296 2148 Size: 99 12 1185 852 Fragments arranged by size: 1185 852 99 12 EcoRV GAT'ATC Cuts at: 0 783 2148 Size: 783 1365 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 421 967 2148 Size: 421 546 1181 Fragments arranged by size: 1181 546 421 HaeII r_GCGC'y Cuts at: 0 805 2148 Size: 805 1343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 62 94 2148 Size: 62 32 2054 Fragments arranged by size: 2054 62 32 HindIII A'AGCT_T Cuts at: 0 2004 2148 Size: 2004 144 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1589 2148 Size: 1589 559 MscI TGG'CCA Cuts at: 0 4 2148 Size: 4 2144 MslI CAynn'nnrTG Cuts at: 0 272 2148 Size: 272 1876 MspA1I CmG'CkG Cuts at: 0 124 172 664 1023 2148 Size: 124 48 492 359 1125 Fragments arranged by size: 1125 492 359 124 48 NsiI A_TGCA'T Cuts at: 0 1383 1395 2148 Size: 1383 12 753 Fragments arranged by size: 1383 753 12 NspI r_CATG'y Cuts at: 0 223 1393 2148 Size: 223 1170 755 Fragments arranged by size: 1170 755 223 PflMI CCAn_nnn'nTGG Cuts at: 0 18 2148 Size: 18 2130 PpuMI rG'GwC_Cy Cuts at: 0 99 1296 2148 Size: 99 1197 852 Fragments arranged by size: 1197 852 99 PvuII CAG'CTG Cuts at: 0 664 1023 2148 Size: 664 359 1125 Fragments arranged by size: 1125 664 359 SacI G_AGCT'C Cuts at: 0 593 1133 2148 Size: 593 540 1015 Fragments arranged by size: 1015 593 540 SapI GCTCTTCn'nnn_ Cuts at: 0 1121 2148 Size: 1121 1027 ScaI AGT'ACT Cuts at: 0 1952 1960 2148 Size: 1952 8 188 Fragments arranged by size: 1952 188 8 SmaI CCC'GGG Cuts at: 0 1012 1684 2148 Size: 1012 672 464 Fragments arranged by size: 1012 672 464 SmlI C'TyrA_G Cuts at: 0 1036 1591 1954 2148 Size: 1036 555 363 194 Fragments arranged by size: 1036 555 363 194 StyI C'CwwG_G Cuts at: 0 556 1477 2148 Size: 556 921 671 Fragments arranged by size: 921 671 556 SwaI ATTT'AAAT Cuts at: 0 975 2148 Size: 975 1173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 684 2148 Size: 684 1464 TatI w'GTAC_w Cuts at: 0 1950 1958 2148 Size: 1950 8 190 Fragments arranged by size: 1950 190 8 XmaI C'CCGG_G Cuts at: 0 1010 1682 2148 Size: 1010 672 466 Fragments arranged by size: 1010 672 466 XmnI GAAnn'nnTTC Cuts at: 0 841 2148 Size: 841 1307 Enzymes that do cut: AarI AcuI AfeI AflII AflIII AlwNI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NsiI NspI PflMI PpuMI PvuII SacI SapI ScaI SmaI SmlI StyI SwaI TaqII TatI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI BaeI BbeI BbvCI BcgI BclI BlpI BmgBI BmtI BsaAI BsaHI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI CspCI DrdI EagI EciI EcoRI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI TstI Tth111I XbaI XcmI XhoI ZraI