Restriction map for
pF1KE0321
(Linear) (Six-base) MAPSORT of: KIEE0321.seq Check: 787 from: 1 to: 2658
KIEE0321.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1659 2658
Size: 1659 999
AccI GT'mk_AC
Cuts at: 0 2214 2658
Size: 2214 444
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 967 1178 2396 2658
Size: 213 754 211 1218 262
Fragments arranged by size:
1218 754 262 213 211
AfeI AGC'GCT
Cuts at: 0 520 2658
Size: 520 2138
AflII C'TTAA_G
Cuts at: 0 738 2658
Size: 738 1920
AflIII A'CryG_T
Cuts at: 0 164 1785 2658
Size: 164 1621 873
Fragments arranged by size:
1621 873 164
AleI CACnn'nnGTG
Cuts at: 0 1363 2658
Size: 1363 1295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1322 2658
Size: 1322 1336
AlwNI CAG_nnn'CTG
Cuts at: 0 488 498 945 1061 1249 2127 2284 2658
Size: 488 10 447 116 188 878 157 374
Fragments arranged by size:
878 488 447 374 188 157 116 10
ApaI G_GGCC'C
Cuts at: 0 831 2080 2658
Size: 831 1249 578
Fragments arranged by size:
1249 831 578
ApaLI G'TGCA_C
Cuts at: 0 1012 2658
Size: 1012 1646
ApoI r'AATT_y
Cuts at: 0 196 1401 1968 1986 2658
Size: 196 1205 567 18 672
Fragments arranged by size:
1205 672 567 196 18
AvaI C'yCGr_G
Cuts at: 0 824 1457 2651 2658
Size: 824 633 1194 7
Fragments arranged by size:
1194 824 633 7
BamHI G'GATC_C
Cuts at: 0 2467 2658
Size: 2467 191
BanII G_rGCy'C
Cuts at: 0 584 665 831 2044 2080 2093 2209 2325
Size: 584 81 166 1213 36 13 116 116
Cuts at: 2325 2380 2658
Size: 55 278
Fragments arranged by size:
1213 584 278 166 116 116 81 55
36 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 774 2658
Size: 774 1884
BciVI GTATCCnnnnn_n'
Cuts at: 0 552 2658
Size: 552 2106
BglI GCCn_nnn'nGGC
Cuts at: 0 345 2025 2511 2658
Size: 345 1680 486 147
Fragments arranged by size:
1680 486 345 147
BglII A'GATC_T
Cuts at: 0 615 2658
Size: 615 2043
Bme1580I G_kGCm'C
Cuts at: 0 831 1016 1136 1917 2080 2658
Size: 831 185 120 781 163 578
Fragments arranged by size:
831 781 578 185 163 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 1049 2658
Size: 1049 1609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1626 2288 2339 2479 2658
Size: 1114 512 662 51 140 179
Fragments arranged by size:
1114 662 512 179 140 51
Bpu10I CC'TnA_GC
Cuts at: 0 1807 2658
Size: 1807 851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1926 2658
Size: 1926 732
BsaI GGTCTCn'nnnn_
Cuts at: 0 362 1454 2658
Size: 362 1092 1204
Fragments arranged by size:
1204 1092 362
BsaAI yAC'GTr
Cuts at: 0 63 919 2658
Size: 63 856 1739
Fragments arranged by size:
1739 856 63
BsaHI Gr'CG_yC
Cuts at: 0 115 2658
Size: 115 2543
BsaWI w'CCGG_w
Cuts at: 0 525 2658
Size: 525 2133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 460 490 2631 2658
Size: 460 30 2141 27
Fragments arranged by size:
2141 460 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2181 2658
Size: 2181 477
BseYI C'CCAG_C
Cuts at: 0 353 1993 2073 2163 2184 2202 2463 2658
Size: 353 1640 80 90 21 18 261 195
Fragments arranged by size:
1640 353 261 195 90 80 21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 2658
Size: 949 1709
BsiEI CG_ry'CG
Cuts at: 0 1497 2658
Size: 1497 1161
BsiHKAI G_wGCw'C
Cuts at: 0 584 1016 1049 1360 1748 2325 2658
Size: 584 432 33 311 388 577 333
Fragments arranged by size:
584 577 432 388 333 311 33
BsmI GAATG_Cn'
Cuts at: 0 251 362 2658
Size: 251 111 2296
Fragments arranged by size:
2296 251 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 388 2103 2658
Size: 388 1715 555
Fragments arranged by size:
1715 555 388
Bsp1286I G_dGCh'C
Cuts at: 0 584 665 831 1016 1049 1136 1360 1748
Size: 584 81 166 185 33 87 224 388
Cuts at: 1748 1917 2044 2080 2093 2209 2325 2380 2658
Size: 169 127 36 13 116 116 55 278
Fragments arranged by size:
584 388 278 224 185 169 166 127
116 116 87 81 55 36 33 13
BspEI T'CCGG_A
Cuts at: 0 525 2658
Size: 525 2133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1304 1659 2658
Size: 1304 355 999
Fragments arranged by size:
1304 999 355
BsrBI CCG'CTC
Cuts at: 0 2519 2658
Size: 2519 139
BsrDI GCAATG_nn'
Cuts at: 0 134 1688 2658
Size: 134 1554 970
Fragments arranged by size:
1554 970 134
BsrFI r'CCGG_y
Cuts at: 0 371 1629 1930 1942 2658
Size: 371 1258 301 12 716
Fragments arranged by size:
1258 716 371 301 12
BstBI TT'CG_AA
Cuts at: 0 1851 2658
Size: 1851 807
BstEII G'GTnAC_C
Cuts at: 0 2058 2658
Size: 2058 600
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1603 2658
Size: 1603 1055
BstYI r'GATC_y
Cuts at: 0 615 2467 2658
Size: 615 1852 191
Fragments arranged by size:
1852 615 191
Bsu36I CC'TnA_GG
Cuts at: 0 1518 2495 2606 2658
Size: 1518 977 111 52
Fragments arranged by size:
1518 977 111 52
BtgI C'CryG_G
Cuts at: 0 438 639 1720 2509 2658
Size: 438 201 1081 789 149
Fragments arranged by size:
1081 789 438 201 149
BtsI GCAGTG_nn'
Cuts at: 0 334 2457 2658
Size: 334 2123 201
Fragments arranged by size:
2123 334 201
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1254 1289 2658
Size: 1254 35 1369
Fragments arranged by size:
1369 1254 35
DraIII CAC_nnn'GTG
Cuts at: 0 1011 2070 2658
Size: 1011 1059 588
Fragments arranged by size:
1059 1011 588
EaeI y'GGCC_r
Cuts at: 0 346 971 1473 1494 1685 2658
Size: 346 625 502 21 191 973
Fragments arranged by size:
973 625 502 346 191 21
EagI C'GGCC_G
Cuts at: 0 1494 2658
Size: 1494 1164
EarI CTCTTCn'nnn_
Cuts at: 0 147 853 1232 1834 2415 2658
Size: 147 706 379 602 581 243
Fragments arranged by size:
706 602 581 379 243 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1723 2658
Size: 1723 935
EcoICRI GAG'CTC
Cuts at: 0 582 2323 2658
Size: 582 1741 335
Fragments arranged by size:
1741 582 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 967 1114 1178 1626 2288 2339 2396
Size: 213 754 147 64 448 662 51 57
Cuts at: 2396 2479 2658
Size: 83 179
Fragments arranged by size:
754 662 448 213 179 147 83 64
57 51
EcoO109I rG'GnC_Cy
Cuts at: 0 1845 2077 2416 2504 2590 2658
Size: 1845 232 339 88 86 68
Fragments arranged by size:
1845 339 232 88 86 68
EcoRI G'AATT_C
Cuts at: 0 1401 1986 2658
Size: 1401 585 672
Fragments arranged by size:
1401 672 585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1012 2658
Size: 1012 1646
FspI TGC'GCA
Cuts at: 0 331 2658
Size: 331 2327
FspAI rTGC'GCAy
Cuts at: 0 331 2658
Size: 331 2327
HaeII r_GCGC'y
Cuts at: 0 522 2658
Size: 522 2136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 754 786 1462 1494 2307 2339
Size: 142 32 580 32 676 32 813 32
Cuts at: 2339 2452 2484 2551 2583 2631 2658
Size: 113 32 67 32 48 27
Fragments arranged by size:
813 676 580 142 113 67 48 32
32 32 32 32 32 27
HincII GTy'rAC
Cuts at: 0 1833 2658
Size: 1833 825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 834 2658
Size: 185 649 1824
Fragments arranged by size:
1824 649 185
MscI TGG'CCA
Cuts at: 0 1475 1687 2658
Size: 1475 212 971
Fragments arranged by size:
1475 971 212
MslI CAynn'nnrTG
Cuts at: 0 1304 1363 1545 1601 2658
Size: 1304 59 182 56 1057
Fragments arranged by size:
1304 1057 182 59 56
MspA1I CmG'CkG
Cuts at: 0 1141 2658
Size: 1141 1517
NaeI GCC'GGC
Cuts at: 0 1631 2658
Size: 1631 1027
NcoI C'CATG_G
Cuts at: 0 639 2658
Size: 639 2019
NgoMIV G'CCGG_C
Cuts at: 0 1629 2658
Size: 1629 1029
NspI r_CATG'y
Cuts at: 0 1309 1377 1789 2658
Size: 1309 68 412 869
Fragments arranged by size:
1309 869 412 68
PasI CC'CwG_GG
Cuts at: 0 309 757 2658
Size: 309 448 1901
Fragments arranged by size:
1901 448 309
PciI A'CATG_T
Cuts at: 0 1785 2658
Size: 1785 873
PflMI CCAn_nnn'nTGG
Cuts at: 0 167 315 2658
Size: 167 148 2343
Fragments arranged by size:
2343 167 148
PfoI T'CCnGG_A
Cuts at: 0 2569 2658
Size: 2569 89
PmlI CAC'GTG
Cuts at: 0 63 919 2658
Size: 63 856 1739
Fragments arranged by size:
1739 856 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1178 1210 2658
Size: 1178 32 1448
Fragments arranged by size:
1448 1178 32
PpuMI rG'GwC_Cy
Cuts at: 0 2590 2658
Size: 2590 68
PspOMI G'GGCC_C
Cuts at: 0 827 2076 2658
Size: 827 1249 582
Fragments arranged by size:
1249 827 582
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 925 957 2658
Size: 925 32 1701
Fragments arranged by size:
1701 925 32
PstI C_TGCA'G
Cuts at: 0 2025 2145 2658
Size: 2025 120 513
Fragments arranged by size:
2025 513 120
PvuII CAG'CTG
Cuts at: 0 1141 2658
Size: 1141 1517
SacI G_AGCT'C
Cuts at: 0 584 2325 2658
Size: 584 1741 333
Fragments arranged by size:
1741 584 333
SanDI GG'GwC_CC
Cuts at: 0 2590 2658
Size: 2590 68
SapI GCTCTTCn'nnn_
Cuts at: 0 2415 2658
Size: 2415 243
SbfI CC_TGCA'GG
Cuts at: 0 2025 2145 2658
Size: 2025 120 513
Fragments arranged by size:
2025 513 120
SfcI C'TryA_G
Cuts at: 0 480 2021 2141 2547 2658
Size: 480 1541 120 406 111
Fragments arranged by size:
1541 480 406 120 111
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2511 2658
Size: 2511 147
SmaI CCC'GGG
Cuts at: 0 826 2653 2658
Size: 826 1827 5
Fragments arranged by size:
1827 826 5
SmlI C'TyrA_G
Cuts at: 0 738 1905 2658
Size: 738 1167 753
Fragments arranged by size:
1167 753 738
SpeI A'CTAG_T
Cuts at: 0 2173 2658
Size: 2173 485
StuI AGG'CCT
Cuts at: 0 1805 2036 2658
Size: 1805 231 622
Fragments arranged by size:
1805 622 231
StyI C'CwwG_G
Cuts at: 0 639 2594 2658
Size: 639 1955 64
Fragments arranged by size:
1955 639 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 770 1375 1816 1875 2077 2658
Size: 770 605 441 59 202 581
Fragments arranged by size:
770 605 581 441 202 59
TatI w'GTAC_w
Cuts at: 0 750 1734 2658
Size: 750 984 924
Fragments arranged by size:
984 924 750
XbaI T'CTAG_A
Cuts at: 0 669 2658
Size: 669 1989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1650 1695 2658
Size: 1650 45 963
Fragments arranged by size:
1650 963 45
XmaI C'CCGG_G
Cuts at: 0 824 2651 2658
Size: 824 1827 7
Fragments arranged by size:
1827 824 7
XmnI GAAnn'nnTTC
Cuts at: 0 362 2658
Size: 362 2296
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanII BbsI
BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuII SacI SanDI SapI SbfI
SfcI SfiI SmaI SmlI SpeI StuI StyI TaqII
TatI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AloI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI
BfrBI BlpI BmgBI BmtI BplI BsaBI BsiWI BspHI
BsrGI BssHII BssSI BstAPI BstZ17I BtgZI ClaI DraI
DrdI EcoNI EcoRV FseI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PmeI PshAI PsiI PspXI PvuI RsrII SacII
SalI ScaI SexAI SfoI SgfI SgrAI SnaBI SphI
SrfI SspI SwaI TstI Tth111I XhoI ZraI