Restriction map for
pF1KE0324
(Linear) (Six-base) MAPSORT of: KIEE0324.seq Check: 5269 from: 1 to: 1854
KIEE0324.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 412 1854
Size: 412 1442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1172 1288 1469 1854
Size: 475 697 116 181 385
Fragments arranged by size:
697 475 385 181 116
ApaI G_GGCC'C
Cuts at: 0 515 1854
Size: 515 1339
ApoI r'AATT_y
Cuts at: 0 82 1343 1438 1854
Size: 82 1261 95 416
Fragments arranged by size:
1261 416 95 82
AvaI C'yCGr_G
Cuts at: 0 104 1854
Size: 104 1750
BamHI G'GATC_C
Cuts at: 0 17 1854
Size: 17 1837
BanI G'GyrC_C
Cuts at: 0 1127 1854
Size: 1127 727
BanII G_rGCy'C
Cuts at: 0 473 515 1768 1854
Size: 473 42 1253 86
Fragments arranged by size:
1253 473 86 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 1854
Size: 55 1799
BciVI GTATCCnnnnn_n'
Cuts at: 0 1745 1854
Size: 1745 109
BclI T'GATC_A
Cuts at: 0 588 1854
Size: 588 1266
BfrBI ATG'CAT
Cuts at: 0 913 1854
Size: 913 941
Bme1580I G_kGCm'C
Cuts at: 0 515 1854
Size: 515 1339
BmgBI CAC'GTC
Cuts at: 0 12 1854
Size: 12 1842
BmrI ACTGGGnnnn_n'
Cuts at: 0 1086 1688 1854
Size: 1086 602 166
Fragments arranged by size:
1086 602 166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1854
Size: 697 1157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1166 1744 1854
Size: 653 513 578 110
Fragments arranged by size:
653 578 513 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 1854
Size: 379 1475
BsaAI yAC'GTr
Cuts at: 0 1743 1854
Size: 1743 111
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 656 1854
Size: 544 112 1198
Fragments arranged by size:
1198 544 112
BsiHKAI G_wGCw'C
Cuts at: 0 473 1768 1854
Size: 473 1295 86
Fragments arranged by size:
1295 473 86
BsmI GAATG_Cn'
Cuts at: 0 261 1854
Size: 261 1593
Bsp1286I G_dGCh'C
Cuts at: 0 473 515 1768 1854
Size: 473 42 1253 86
Fragments arranged by size:
1253 473 86 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1232 1598 1854
Size: 1232 366 256
Fragments arranged by size:
1232 366 256
BsrGI T'GTAC_A
Cuts at: 0 1119 1829 1854
Size: 1119 710 25
Fragments arranged by size:
1119 710 25
BstYI r'GATC_y
Cuts at: 0 17 1854
Size: 17 1837
BstZ17I GTA'TAC
Cuts at: 0 413 1854
Size: 413 1441
DraI TTT'AAA
Cuts at: 0 579 999 1854
Size: 579 420 855
Fragments arranged by size:
855 579 420
EaeI y'GGCC_r
Cuts at: 0 1727 1854
Size: 1727 127
EarI CTCTTCn'nnn_
Cuts at: 0 86 1316 1854
Size: 86 1230 538
Fragments arranged by size:
1230 538 86
EcoICRI GAG'CTC
Cuts at: 0 471 1766 1854
Size: 471 1295 88
Fragments arranged by size:
1295 471 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 697 1172 1288 1469 1854
Size: 475 222 475 116 181 385
Fragments arranged by size:
475 475 385 222 181 116
EcoO109I rG'GnC_Cy
Cuts at: 0 511 512 1854
Size: 511 1 1342
Fragments arranged by size:
1342 511 1
EcoRV GAT'ATC
Cuts at: 0 1425 1704 1854
Size: 1425 279 150
Fragments arranged by size:
1425 279 150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 514 1557 1722 1854
Size: 514 1043 165 132
Fragments arranged by size:
1043 514 165 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 1162 1194 1345 1377 1854
Size: 51 32 1079 32 151 32 477
Fragments arranged by size:
1079 477 151 51 32 32 32
HincII GTy'rAC
Cuts at: 0 252 737 1854
Size: 252 485 1117
Fragments arranged by size:
1117 485 252
HindIII A'AGCT_T
Cuts at: 0 1184 1854
Size: 1184 670
HpaI GTT'AAC
Cuts at: 0 252 1854
Size: 252 1602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1854
Size: 178 1676
MscI TGG'CCA
Cuts at: 0 1729 1854
Size: 1729 125
MslI CAynn'nnrTG
Cuts at: 0 710 1854
Size: 710 1144
MspA1I CmG'CkG
Cuts at: 0 130 1125 1192 1854
Size: 130 995 67 662
Fragments arranged by size:
995 662 130 67
NsiI A_TGCA'T
Cuts at: 0 915 1854
Size: 915 939
PflMI CCAn_nnn'nTGG
Cuts at: 0 1775 1854
Size: 1775 79
PmlI CAC'GTG
Cuts at: 0 1743 1854
Size: 1743 111
PspOMI G'GGCC_C
Cuts at: 0 511 1854
Size: 511 1343
PstI C_TGCA'G
Cuts at: 0 1229 1639 1854
Size: 1229 410 215
Fragments arranged by size:
1229 410 215
PvuII CAG'CTG
Cuts at: 0 130 1192 1854
Size: 130 1062 662
Fragments arranged by size:
1062 662 130
SacI G_AGCT'C
Cuts at: 0 473 1768 1854
Size: 473 1295 86
Fragments arranged by size:
1295 473 86
SapI GCTCTTCn'nnn_
Cuts at: 0 1316 1854
Size: 1316 538
SfcI C'TryA_G
Cuts at: 0 506 1225 1635 1854
Size: 506 719 410 219
Fragments arranged by size:
719 506 410 219
SmaI CCC'GGG
Cuts at: 0 106 1854
Size: 106 1748
SmlI C'TyrA_G
Cuts at: 0 668 1181 1759 1854
Size: 668 513 578 95
Fragments arranged by size:
668 578 513 95
SspI AAT'ATT
Cuts at: 0 341 856 1138 1163 1854
Size: 341 515 282 25 691
Fragments arranged by size:
691 515 341 282 25
StyI C'CwwG_G
Cuts at: 0 1018 1854
Size: 1018 836
TatI w'GTAC_w
Cuts at: 0 518 1119 1829 1854
Size: 518 601 710 25
Fragments arranged by size:
710 601 518 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 758 790 1854
Size: 758 32 1064
Fragments arranged by size:
1064 758 32
XmaI C'CCGG_G
Cuts at: 0 104 1854
Size: 104 1750
XmnI GAAnn'nnTTC
Cuts at: 0 83 95 200 1854
Size: 83 12 105 1654
Fragments arranged by size:
1654 105 83 12
Enzymes that do cut:
AccI AcuI ApaI ApoI AvaI BamHI BanI BanII
BbsI BciVI BclI BfrBI Bme1580I BmgBI BmrI BpmI
BpuEI BsaI BsaAI BseRI BsiHKAI BsmI Bsp1286I BspMI
BsrGI BstYI BstZ17I DraI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRV FalI Hin4I HincII HindIII HpaI MmeI
MscI MslI MspA1I NsiI PflMI PmlI PspOMI PstI
PvuII SacI SapI SfcI SmaI SmlI SspI StyI
TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BglI BglII
BlpI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoRI FseI FspI FspAI HaeII
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI ZraI