Restriction map for
pF1KE0325
(Linear) (Six-base) MAPSORT of: KIEE0325.seq Check: 203 from: 1 to: 1512
KIEE0325.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 353 1512
Size: 353 1159
AatII G_ACGT'C
Cuts at: 0 1091 1512
Size: 1091 421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 797 1512
Size: 741 56 715
Fragments arranged by size:
741 715 56
AlwNI CAG_nnn'CTG
Cuts at: 0 1185 1512
Size: 1185 327
ApaI G_GGCC'C
Cuts at: 0 112 1512
Size: 112 1400
AvaI C'yCGr_G
Cuts at: 0 901 1512
Size: 901 611
BanI G'GyrC_C
Cuts at: 0 6 36 1306 1512
Size: 6 30 1270 206
Fragments arranged by size:
1270 206 30 6
BanII G_rGCy'C
Cuts at: 0 112 929 1512
Size: 112 817 583
Fragments arranged by size:
817 583 112
BbeI G_GCGC'C
Cuts at: 0 10 1512
Size: 10 1502
BbsI GAAGACnn'nnnn_
Cuts at: 0 199 1512
Size: 199 1313
BbvCI CC'TCA_GC
Cuts at: 0 223 1512
Size: 223 1289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 128 1512
Size: 94 34 1384
Fragments arranged by size:
1384 94 34
Bme1580I G_kGCm'C
Cuts at: 0 41 112 716 1145 1512
Size: 41 71 604 429 367
Fragments arranged by size:
604 429 367 71 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 1485 1512
Size: 1485 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 561 1512
Size: 561 951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 436 553 630 1512
Size: 312 124 117 77 882
Fragments arranged by size:
882 312 124 117 77
Bpu10I CC'TnA_GC
Cuts at: 0 223 1512
Size: 223 1289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 913 1476 1512
Size: 337 576 563 36
Fragments arranged by size:
576 563 337 36
BsaAI yAC'GTr
Cuts at: 0 711 765 1512
Size: 711 54 747
Fragments arranged by size:
747 711 54
BsaHI Gr'CG_yC
Cuts at: 0 7 1088 1512
Size: 7 1081 424
Fragments arranged by size:
1081 424 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 550 1512
Size: 550 962
BseYI C'CCAG_C
Cuts at: 0 157 393 829 1512
Size: 157 236 436 683
Fragments arranged by size:
683 436 236 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 807 808 1042 1512
Size: 98 709 1 234 470
Fragments arranged by size:
709 470 234 98 1
BsiHKAI G_wGCw'C
Cuts at: 0 929 1512
Size: 929 583
Bsp1286I G_dGCh'C
Cuts at: 0 41 112 716 929 1145 1512
Size: 41 71 604 213 216 367
Fragments arranged by size:
604 367 216 213 71 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 786 1265 1512
Size: 353 433 479 247
Fragments arranged by size:
479 433 353 247
BsrDI GCAATG_nn'
Cuts at: 0 234 1512
Size: 234 1278
BsrFI r'CCGG_y
Cuts at: 0 1493 1512
Size: 1493 19
BsrGI T'GTAC_A
Cuts at: 0 482 986 1512
Size: 482 504 526
Fragments arranged by size:
526 504 482
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1284 1407 1512
Size: 1284 123 105
Fragments arranged by size:
1284 123 105
BstYI r'GATC_y
Cuts at: 0 1390 1512
Size: 1390 122
Bsu36I CC'TnA_GG
Cuts at: 0 360 806 1482 1512
Size: 360 446 676 30
Fragments arranged by size:
676 446 360 30
BtgI C'CryG_G
Cuts at: 0 83 269 1370 1512
Size: 83 186 1101 142
Fragments arranged by size:
1101 186 142 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 960 1512
Size: 960 552
BtsI GCAGTG_nn'
Cuts at: 0 721 1512
Size: 721 791
EaeI y'GGCC_r
Cuts at: 0 272 440 1112 1512
Size: 272 168 672 400
Fragments arranged by size:
672 400 272 168
EarI CTCTTCn'nnn_
Cuts at: 0 1018 1512
Size: 1018 494
EcoICRI GAG'CTC
Cuts at: 0 927 1512
Size: 927 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 436 553 630 741 797 1512
Size: 312 124 117 77 111 56 715
Fragments arranged by size:
715 312 124 117 111 77 56
EcoO109I rG'GnC_Cy
Cuts at: 0 108 109 413 933 1512
Size: 108 1 304 520 579
Fragments arranged by size:
579 520 304 108 1
HaeII r_GCGC'y
Cuts at: 0 10 1512
Size: 10 1502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1465 1497 1512
Size: 1465 32 15
Fragments arranged by size:
1465 32 15
HincII GTy'rAC
Cuts at: 0 308 1512
Size: 308 1204
KasI G'GCGC_C
Cuts at: 0 6 1512
Size: 6 1506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 1028 1512
Size: 989 39 484
Fragments arranged by size:
989 484 39
MscI TGG'CCA
Cuts at: 0 274 442 1512
Size: 274 168 1070
Fragments arranged by size:
1070 274 168
MslI CAynn'nnrTG
Cuts at: 0 82 1512
Size: 82 1430
MspA1I CmG'CkG
Cuts at: 0 245 313 393 650 792 1208 1512
Size: 245 68 80 257 142 416 304
Fragments arranged by size:
416 304 257 245 142 80 68
NaeI GCC'GGC
Cuts at: 0 1495 1512
Size: 1495 17
NarI GG'CG_CC
Cuts at: 0 7 1512
Size: 7 1505
NcoI C'CATG_G
Cuts at: 0 83 269 1512
Size: 83 186 1243
Fragments arranged by size:
1243 186 83
NgoMIV G'CCGG_C
Cuts at: 0 1493 1512
Size: 1493 19
NspI r_CATG'y
Cuts at: 0 1222 1512
Size: 1222 290
PmlI CAC'GTG
Cuts at: 0 711 765 1512
Size: 711 54 747
Fragments arranged by size:
747 711 54
PpuMI rG'GwC_Cy
Cuts at: 0 413 933 1512
Size: 413 520 579
Fragments arranged by size:
579 520 413
PshAI GACnn'nnGTC
Cuts at: 0 313 1126 1512
Size: 313 813 386
Fragments arranged by size:
813 386 313
PspOMI G'GGCC_C
Cuts at: 0 108 1512
Size: 108 1404
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 553 585 1512
Size: 553 32 927
Fragments arranged by size:
927 553 32
PstI C_TGCA'G
Cuts at: 0 17 797 1512
Size: 17 780 715
Fragments arranged by size:
780 715 17
PvuII CAG'CTG
Cuts at: 0 313 393 792 1208 1512
Size: 313 80 399 416 304
Fragments arranged by size:
416 399 313 304 80
SacI G_AGCT'C
Cuts at: 0 929 1512
Size: 929 583
SanDI GG'GwC_CC
Cuts at: 0 413 1512
Size: 413 1099
SexAI A'CCwGG_T
Cuts at: 0 572 1512
Size: 572 940
SfcI C'TryA_G
Cuts at: 0 13 145 793 1065 1512
Size: 13 132 648 272 447
Fragments arranged by size:
648 447 272 132 13
SfoI GGC'GCC
Cuts at: 0 8 1512
Size: 8 1504
SmlI C'TyrA_G
Cuts at: 0 352 928 1455 1512
Size: 352 576 527 57
Fragments arranged by size:
576 527 352 57
StyI C'CwwG_G
Cuts at: 0 83 269 1512
Size: 83 186 1243
Fragments arranged by size:
1243 186 83
TatI w'GTAC_w
Cuts at: 0 482 986 995 1512
Size: 482 504 9 517
Fragments arranged by size:
517 504 482 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 873 1512
Size: 873 639
ZraI GAC'GTC
Cuts at: 0 1089 1512
Size: 1089 423
Enzymes that do cut:
AarI AatII AcuI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PmlI PpuMI PshAI
PspOMI PsrI PstI PvuII SacI SanDI SexAI SfcI
SfoI SmlI StyI TatI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BsaI BsaBI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PpiI PsiI PspXI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI