Restriction map for
pF1KE0326
(Linear) (Six-base) MAPSORT of: KIEE0326.seq Check: 3928 from: 1 to: 1917
KIEE0326.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1695 1917
Size: 1695 222
AccI GT'mk_AC
Cuts at: 0 1475 1917
Size: 1475 442
Acc65I G'GTAC_C
Cuts at: 0 167 1917
Size: 167 1750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 452 688 1699 1917
Size: 179 273 236 1011 218
Fragments arranged by size:
1011 273 236 218 179
AlwNI CAG_nnn'CTG
Cuts at: 0 285 1632 1680 1917
Size: 285 1347 48 237
Fragments arranged by size:
1347 285 237 48
ApaI G_GGCC'C
Cuts at: 0 798 1917
Size: 798 1119
AvaI C'yCGr_G
Cuts at: 0 520 1238 1917
Size: 520 718 679
Fragments arranged by size:
718 679 520
BanI G'GyrC_C
Cuts at: 0 167 530 784 1089 1917
Size: 167 363 254 305 828
Fragments arranged by size:
828 363 305 254 167
BanII G_rGCy'C
Cuts at: 0 516 798 884 1084 1547 1917
Size: 516 282 86 200 463 370
Fragments arranged by size:
516 463 370 282 200 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 831 958 1917
Size: 831 127 959
Fragments arranged by size:
959 831 127
BbvCI CC'TCA_GC
Cuts at: 0 212 694 889 1917
Size: 212 482 195 1028
Fragments arranged by size:
1028 482 212 195
BfrBI ATG'CAT
Cuts at: 0 1808 1917
Size: 1808 109
BglI GCCn_nnn'nGGC
Cuts at: 0 372 793 869 1917
Size: 372 421 76 1048
Fragments arranged by size:
1048 421 372 76
Bme1580I G_kGCm'C
Cuts at: 0 495 656 798 1094 1917
Size: 495 161 142 296 823
Fragments arranged by size:
823 495 296 161 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 411 1029 1447 1502 1767 1917
Size: 411 618 418 55 265 150
Fragments arranged by size:
618 418 411 265 150 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1395 1917
Size: 1395 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 262 460 490 766 898 1039 1157
Size: 25 237 198 30 276 132 141 118
Cuts at: 1157 1917
Size: 760
Fragments arranged by size:
760 276 237 198 141 132 118 30
25
Bpu10I CC'TnA_GC
Cuts at: 0 212 694 889 1481 1917
Size: 212 482 195 592 436
Fragments arranged by size:
592 482 436 212 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1404 1917
Size: 697 707 513
Fragments arranged by size:
707 697 513
BsaI GGTCTCn'nnnn_
Cuts at: 0 1318 1917
Size: 1318 599
BsaAI yAC'GTr
Cuts at: 0 469 1917
Size: 469 1448
BsaWI w'CCGG_w
Cuts at: 0 927 1917
Size: 927 990
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 458 488 1088 1118 1425 1455 1917
Size: 458 30 600 30 307 30 462
Fragments arranged by size:
600 462 458 307 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 877 1917
Size: 454 423 1040
Fragments arranged by size:
1040 454 423
BseYI C'CCAG_C
Cuts at: 0 66 780 804 1332 1917
Size: 66 714 24 528 585
Fragments arranged by size:
714 585 528 66 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1571 1707 1917
Size: 103 1468 136 210
Fragments arranged by size:
1468 210 136 103
BsiHKAI G_wGCw'C
Cuts at: 0 836 1917
Size: 836 1081
Bsp1286I G_dGCh'C
Cuts at: 0 495 516 656 798 836 884 1084 1094
Size: 495 21 140 142 38 48 200 10
Cuts at: 1094 1547 1917
Size: 453 370
Fragments arranged by size:
495 453 370 200 142 140 48 38
21 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 989 1640 1695 1856 1917
Size: 989 651 55 161 61
Fragments arranged by size:
989 651 161 61 55
BsrDI GCAATG_nn'
Cuts at: 0 1316 1917
Size: 1316 601
BsrFI r'CCGG_y
Cuts at: 0 1616 1917
Size: 1616 301
BsrGI T'GTAC_A
Cuts at: 0 630 1917
Size: 630 1287
BssSI C'ACGA_G
Cuts at: 0 682 1917
Size: 682 1235
BstAPI GCAn_nnn'nTGC
Cuts at: 0 490 1917
Size: 490 1427
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1135 1534 1596 1774 1917
Size: 1135 399 62 178 143
Fragments arranged by size:
1135 399 178 143 62
Bsu36I CC'TnA_GG
Cuts at: 0 7 557 1300 1917
Size: 7 550 743 617
Fragments arranged by size:
743 617 550 7
BtgI C'CryG_G
Cuts at: 0 293 1109 1589 1917
Size: 293 816 480 328
Fragments arranged by size:
816 480 328 293
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 106 1917
Size: 106 1811
BtsI GCAGTG_nn'
Cuts at: 0 265 852 1614 1885 1917
Size: 265 587 762 271 32
Fragments arranged by size:
762 587 271 265 32
DraIII CAC_nnn'GTG
Cuts at: 0 490 1917
Size: 490 1427
DrdI GACnn_nn'nnGTC
Cuts at: 0 1442 1917
Size: 1442 475
EaeI y'GGCC_r
Cuts at: 0 290 737 1106 1466 1739 1917
Size: 290 447 369 360 273 178
Fragments arranged by size:
447 369 360 290 273 178
EarI CTCTTCn'nnn_
Cuts at: 0 221 431 1917
Size: 221 210 1486
Fragments arranged by size:
1486 221 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 179 262 452 460 490 688 766
Size: 25 154 83 190 8 30 198 78
Cuts at: 766 898 1039 1157 1699 1917
Size: 132 141 118 542 218
Fragments arranged by size:
542 218 198 190 154 141 132 118
83 78 30 25 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 478 1285 1917
Size: 478 807 632
Fragments arranged by size:
807 632 478
EcoO109I rG'GnC_Cy
Cuts at: 0 246 659 794 978 1050 1917
Size: 246 413 135 184 72 867
Fragments arranged by size:
867 413 246 184 135 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1529 1561 1917
Size: 595 32 902 32 356
Fragments arranged by size:
902 595 356 32 32
HincII GTy'rAC
Cuts at: 0 446 1917
Size: 446 1471
KpnI G_GTAC'C
Cuts at: 0 171 1917
Size: 171 1746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1426 1917
Size: 977 449 491
Fragments arranged by size:
977 491 449
MscI TGG'CCA
Cuts at: 0 739 1108 1468 1741 1917
Size: 739 369 360 273 176
Fragments arranged by size:
739 369 360 273 176
MslI CAynn'nnrTG
Cuts at: 0 682 1917
Size: 682 1235
MspA1I CmG'CkG
Cuts at: 0 282 1425 1917
Size: 282 1143 492
Fragments arranged by size:
1143 492 282
NaeI GCC'GGC
Cuts at: 0 1618 1917
Size: 1618 299
NcoI C'CATG_G
Cuts at: 0 1109 1589 1917
Size: 1109 480 328
Fragments arranged by size:
1109 480 328
NgoMIV G'CCGG_C
Cuts at: 0 1616 1917
Size: 1616 301
NsiI A_TGCA'T
Cuts at: 0 1810 1917
Size: 1810 107
NspI r_CATG'y
Cuts at: 0 1042 1917
Size: 1042 875
PasI CC'CwG_GG
Cuts at: 0 656 1917
Size: 656 1261
PfoI T'CCnGG_A
Cuts at: 0 476 1766 1917
Size: 476 1290 151
Fragments arranged by size:
1290 476 151
PmlI CAC'GTG
Cuts at: 0 469 1917
Size: 469 1448
PpuMI rG'GwC_Cy
Cuts at: 0 978 1917
Size: 978 939
PspOMI G'GGCC_C
Cuts at: 0 794 1917
Size: 794 1123
PstI C_TGCA'G
Cuts at: 0 548 986 1637 1917
Size: 548 438 651 280
Fragments arranged by size:
651 548 438 280
PvuII CAG'CTG
Cuts at: 0 282 1425 1917
Size: 282 1143 492
Fragments arranged by size:
1143 492 282
SfcI C'TryA_G
Cuts at: 0 544 982 1633 1917
Size: 544 438 651 284
Fragments arranged by size:
651 544 438 284
SmlI C'TyrA_G
Cuts at: 0 712 1383 1917
Size: 712 671 534
Fragments arranged by size:
712 671 534
StuI AGG'CCT
Cuts at: 0 607 1198 1375 1736 1917
Size: 607 591 177 361 181
Fragments arranged by size:
607 591 361 181 177
StyI C'CwwG_G
Cuts at: 0 367 1109 1589 1917
Size: 367 742 480 328
Fragments arranged by size:
742 480 367 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 548 772 881 1917
Size: 548 224 109 1036
Fragments arranged by size:
1036 548 224 109
TatI w'GTAC_w
Cuts at: 0 230 630 1917
Size: 230 400 1287
Fragments arranged by size:
1287 400 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 458 490 1088 1120 1917
Size: 458 32 598 32 797
Fragments arranged by size:
797 598 458 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 398 1795 1917
Size: 171 227 1397 122
Fragments arranged by size:
1397 227 171 122
XmnI GAAnn'nnTTC
Cuts at: 0 955 1226 1917
Size: 955 271 691
Fragments arranged by size:
955 691 271
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BfrBI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PfoI PmlI PpuMI PspOMI PstI
PvuII SfcI SmlI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BglII BlpI
BmgBI BmtI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BssHII BstBI BstEII BstYI BstZ17I
ClaI CspCI DraI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI NarI NdeI NheI NotI NruI PacI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI