Restriction map for
pF1KE0335
(Linear) (Six-base) MAPSORT of: KIEE0335.seq Check: 522 from: 1 to: 2742
KIEE0335.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 422 2742
Size: 422 2320
Acc65I G'GTAC_C
Cuts at: 0 1895 2742
Size: 1895 847
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 455 2408 2580 2742
Size: 409 46 1953 172 162
Fragments arranged by size:
1953 409 172 162 46
AfeI AGC'GCT
Cuts at: 0 2017 2742
Size: 2017 725
AflIII A'CryG_T
Cuts at: 0 1708 2061 2742
Size: 1708 353 681
Fragments arranged by size:
1708 681 353
AhdI GACnn_n'nnGTC
Cuts at: 0 429 2742
Size: 429 2313
AleI CACnn'nnGTG
Cuts at: 0 961 2742
Size: 961 1781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 979 1598 2742
Size: 979 619 1144
Fragments arranged by size:
1144 979 619
AlwNI CAG_nnn'CTG
Cuts at: 0 68 892 931 2742
Size: 68 824 39 1811
Fragments arranged by size:
1811 824 68 39
ApaI G_GGCC'C
Cuts at: 0 925 2742
Size: 925 1817
ApaLI G'TGCA_C
Cuts at: 0 964 2742
Size: 964 1778
ApoI r'AATT_y
Cuts at: 0 218 459 592 601 1787 2742
Size: 218 241 133 9 1186 955
Fragments arranged by size:
1186 955 241 218 133 9
BamHI G'GATC_C
Cuts at: 0 680 2742
Size: 680 2062
BanI G'GyrC_C
Cuts at: 0 1741 1895 2742
Size: 1741 154 847
Fragments arranged by size:
1741 847 154
BanII G_rGCy'C
Cuts at: 0 925 1619 1964 2742
Size: 925 694 345 778
Fragments arranged by size:
925 778 694 345
BbsI GAAGACnn'nnnn_
Cuts at: 0 167 1023 1400 2742
Size: 167 856 377 1342
Fragments arranged by size:
1342 856 377 167
BciVI GTATCCnnnnn_n'
Cuts at: 0 757 1084 2742
Size: 757 327 1658
Fragments arranged by size:
1658 757 327
BclI T'GATC_A
Cuts at: 0 2522 2742
Size: 2522 220
BglII A'GATC_T
Cuts at: 0 570 949 2579 2742
Size: 570 379 1630 163
Fragments arranged by size:
1630 570 379 163
Bme1580I G_kGCm'C
Cuts at: 0 925 968 2742
Size: 925 43 1774
Fragments arranged by size:
1774 925 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 835 919 1832 2281 2733 2742
Size: 835 84 913 449 452 9
Fragments arranged by size:
913 835 452 449 84 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2718 2742
Size: 2718 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1925 2713 2742
Size: 1925 788 29
Fragments arranged by size:
1925 788 29
Bpu10I CC'TnA_GC
Cuts at: 0 1273 2686 2742
Size: 1273 1413 56
Fragments arranged by size:
1413 1273 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 377 1426 1686 2742
Size: 377 1049 260 1056
Fragments arranged by size:
1056 1049 377 260
BsaAI yAC'GTr
Cuts at: 0 2064 2663 2742
Size: 2064 599 79
Fragments arranged by size:
2064 599 79
BsaHI Gr'CG_yC
Cuts at: 0 93 2742
Size: 93 2649
BsaWI w'CCGG_w
Cuts at: 0 294 2742
Size: 294 2448
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 794 824 2742
Size: 794 30 1918
Fragments arranged by size:
1918 794 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 260 544 547 1416 2742
Size: 260 284 3 869 1326
Fragments arranged by size:
1326 869 284 260 3
BseYI C'CCAG_C
Cuts at: 0 2148 2557 2742
Size: 2148 409 185
Fragments arranged by size:
2148 409 185
BsiHKAI G_wGCw'C
Cuts at: 0 968 1202 1610 1619 2742
Size: 968 234 408 9 1123
Fragments arranged by size:
1123 968 408 234 9
BsmI GAATG_Cn'
Cuts at: 0 861 1587 2742
Size: 861 726 1155
Fragments arranged by size:
1155 861 726
Bsp1286I G_dGCh'C
Cuts at: 0 925 968 1202 1610 1619 1964 2742
Size: 925 43 234 408 9 345 778
Fragments arranged by size:
925 778 408 345 234 43 9
BspEI T'CCGG_A
Cuts at: 0 294 2742
Size: 294 2448
BspHI T'CATG_A
Cuts at: 0 2081 2742
Size: 2081 661
BsrDI GCAATG_nn'
Cuts at: 0 2711 2742
Size: 2711 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1144 1269 1572 2487 2742
Size: 1144 125 303 915 255
Fragments arranged by size:
1144 915 303 255 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1188 2742
Size: 1188 1554
BstYI r'GATC_y
Cuts at: 0 570 680 949 1501 1826 2282 2579 2742
Size: 570 110 269 552 325 456 297 163
Fragments arranged by size:
570 552 456 325 297 269 163 110
BtgI C'CryG_G
Cuts at: 0 1926 2742
Size: 1926 816
BtsI GCAGTG_nn'
Cuts at: 0 17 1145 2742
Size: 17 1128 1597
Fragments arranged by size:
1597 1128 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2181 2216 2742
Size: 2181 35 526
Fragments arranged by size:
2181 526 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2728 2742
Size: 2728 14
EaeI y'GGCC_r
Cuts at: 0 1414 1929 2451 2742
Size: 1414 515 522 291
Fragments arranged by size:
1414 522 515 291
EarI CTCTTCn'nnn_
Cuts at: 0 257 434 581 1522 2742
Size: 257 177 147 941 1220
Fragments arranged by size:
1220 941 257 177 147
EcoICRI GAG'CTC
Cuts at: 0 1617 2742
Size: 1617 1125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 455 1925 2408 2580 2713 2742
Size: 409 46 1470 483 172 133 29
Fragments arranged by size:
1470 483 409 172 133 46 29
EcoO109I rG'GnC_Cy
Cuts at: 0 1012 2742
Size: 1012 1730
EcoRV GAT'ATC
Cuts at: 0 612 2742
Size: 612 2130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 441 1236 2742
Size: 441 795 1506
Fragments arranged by size:
1506 795 441
HaeII r_GCGC'y
Cuts at: 0 2019 2742
Size: 2019 723
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 523 555 1991 2023 2238 2270
Size: 415 32 76 32 1436 32 215 32
Cuts at: 2270 2742
Size: 472
Fragments arranged by size:
1436 472 415 215 76 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1291 2742
Size: 1291 1451
KpnI G_GTAC'C
Cuts at: 0 1899 2742
Size: 1899 843
MfeI C'AATT_G
Cuts at: 0 1599 2742
Size: 1599 1143
MscI TGG'CCA
Cuts at: 0 1416 1931 2453 2742
Size: 1416 515 522 289
Fragments arranged by size:
1416 522 515 289
MslI CAynn'nnrTG
Cuts at: 0 674 961 2742
Size: 674 287 1781
Fragments arranged by size:
1781 674 287
MspA1I CmG'CkG
Cuts at: 0 1106 2742
Size: 1106 1636
NspI r_CATG'y
Cuts at: 0 694 2148 2742
Size: 694 1454 594
Fragments arranged by size:
1454 694 594
PasI CC'CwG_GG
Cuts at: 0 465 2742
Size: 465 2277
PflMI CCAn_nnn'nTGG
Cuts at: 0 825 2369 2723 2742
Size: 825 1544 354 19
Fragments arranged by size:
1544 825 354 19
PfoI T'CCnGG_A
Cuts at: 0 2214 2742
Size: 2214 528
PmlI CAC'GTG
Cuts at: 0 2064 2742
Size: 2064 678
PpuMI rG'GwC_Cy
Cuts at: 0 1012 2742
Size: 1012 1730
PsiI TTA'TAA
Cuts at: 0 746 2742
Size: 746 1996
PspOMI G'GGCC_C
Cuts at: 0 921 2742
Size: 921 1821
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2032 2064 2742
Size: 2032 32 678
Fragments arranged by size:
2032 678 32
PstI C_TGCA'G
Cuts at: 0 897 1659 2742
Size: 897 762 1083
Fragments arranged by size:
1083 897 762
SacI G_AGCT'C
Cuts at: 0 1619 2742
Size: 1619 1123
ScaI AGT'ACT
Cuts at: 0 1042 2742
Size: 1042 1700
SexAI A'CCwGG_T
Cuts at: 0 169 2742
Size: 169 2573
SfcI C'TryA_G
Cuts at: 0 44 490 836 893 1655 2351 2742
Size: 44 446 346 57 762 696 391
Fragments arranged by size:
762 696 446 391 346 57 44
SnaBI TAC'GTA
Cuts at: 0 2663 2742
Size: 2663 79
StuI AGG'CCT
Cuts at: 0 2088 2742
Size: 2088 654
StyI C'CwwG_G
Cuts at: 0 1451 2294 2742
Size: 1451 843 448
Fragments arranged by size:
1451 843 448
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2419 2742
Size: 2419 323
TatI w'GTAC_w
Cuts at: 0 902 1040 2272 2742
Size: 902 138 1232 470
Fragments arranged by size:
1232 902 470 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 590 622 2053 2085 2254 2286 2742
Size: 590 32 1431 32 169 32 456
Fragments arranged by size:
1431 590 456 169 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2565 2632 2688 2742
Size: 2565 67 56 54
Fragments arranged by size:
2565 67 56 54
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbsI
BciVI BclI BglII Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrDI BstAPI BstXI BstYI
BtgI BtsI CspCI DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HindIII KpnI MfeI
MscI MslI MspA1I NspI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PsrI PstI SacI ScaI SexAI
SfcI SnaBI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AloI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BfrBI
BglI BlpI BmgBI BmtI BpuEI BsaBI BsgI BsiEI
BsiWI BsmBI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII
EagI EciI EcoNI EcoRI FseI FspI FspAI HincII
HpaI KasI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PspXI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI