Restriction map for
pF1KE0336
(Linear) (Six-base) MAPSORT of: KIEE0336.seq Check: 2935 from: 1 to: 1464
KIEE0336.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1427 1464
Size: 1427 37
AatII G_ACGT'C
Cuts at: 0 1166 1464
Size: 1166 298
AccI GT'mk_AC
Cuts at: 0 851 1464
Size: 851 613
AleI CACnn'nnGTG
Cuts at: 0 94 1375 1464
Size: 94 1281 89
Fragments arranged by size:
1281 94 89
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 1464
Size: 739 32 693
Fragments arranged by size:
739 693 32
AlwNI CAG_nnn'CTG
Cuts at: 0 980 1134 1232 1464
Size: 980 154 98 232
Fragments arranged by size:
980 232 154 98
ApaI G_GGCC'C
Cuts at: 0 510 1464
Size: 510 954
AvaI C'yCGr_G
Cuts at: 0 205 1464
Size: 205 1259
BamHI G'GATC_C
Cuts at: 0 650 1464
Size: 650 814
BanI G'GyrC_C
Cuts at: 0 1146 1464
Size: 1146 318
BanII G_rGCy'C
Cuts at: 0 510 627 1319 1464
Size: 510 117 692 145
Fragments arranged by size:
692 510 145 117
BglI GCCn_nnn'nGGC
Cuts at: 0 376 484 643 1464
Size: 376 108 159 821
Fragments arranged by size:
821 376 159 108
Bme1580I G_kGCm'C
Cuts at: 0 510 1151 1464
Size: 510 641 313
Fragments arranged by size:
641 510 313
BmgBI CAC'GTC
Cuts at: 0 1182 1464
Size: 1182 282
BmrI ACTGGGnnnn_n'
Cuts at: 0 771 895 1464
Size: 771 124 569
Fragments arranged by size:
771 569 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 216 1254 1462 1464
Size: 175 41 1038 208 2
Fragments arranged by size:
1038 208 175 41 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1464
Size: 193 1271
BsaI GGTCTCn'nnnn_
Cuts at: 0 1037 1464
Size: 1037 427
BsaAI yAC'GTr
Cuts at: 0 249 1464
Size: 249 1215
BsaHI Gr'CG_yC
Cuts at: 0 1118 1163 1464
Size: 1118 45 301
Fragments arranged by size:
1118 301 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 739 769 1464
Size: 739 30 695
Fragments arranged by size:
739 695 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 314 1464
Size: 115 199 1150
Fragments arranged by size:
1150 199 115
BseYI C'CCAG_C
Cuts at: 0 39 392 1464
Size: 39 353 1072
Fragments arranged by size:
1072 353 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1120 1156 1279 1464
Size: 414 706 36 123 185
Fragments arranged by size:
706 414 185 123 36
BsiEI CG_ry'CG
Cuts at: 0 1459 1464
Size: 1459 5
BsmI GAATG_Cn'
Cuts at: 0 1068 1464
Size: 1068 396
BsmBI CGTCTCn'nnnn_
Cuts at: 0 348 1464
Size: 348 1116
Bsp1286I G_dGCh'C
Cuts at: 0 510 627 1151 1319 1464
Size: 510 117 524 168 145
Fragments arranged by size:
524 510 168 145 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 140 998 1427 1464
Size: 122 18 858 429 37
Fragments arranged by size:
858 429 122 37 18
BsrDI GCAATG_nn'
Cuts at: 0 1130 1464
Size: 1130 334
BsrGI T'GTAC_A
Cuts at: 0 1019 1464
Size: 1019 445
BssSI C'ACGA_G
Cuts at: 0 499 1054 1464
Size: 499 555 410
Fragments arranged by size:
555 499 410
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1134 1464
Size: 1134 330
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 1362 1464
Size: 453 909 102
Fragments arranged by size:
909 453 102
BstYI r'GATC_y
Cuts at: 0 650 1464
Size: 650 814
BtgI C'CryG_G
Cuts at: 0 239 371 807 1079 1464
Size: 239 132 436 272 385
Fragments arranged by size:
436 385 272 239 132
BtsI GCAGTG_nn'
Cuts at: 0 230 439 1464
Size: 230 209 1025
Fragments arranged by size:
1025 230 209
DraIII CAC_nnn'GTG
Cuts at: 0 1056 1464
Size: 1056 408
DrdI GACnn_nn'nnGTC
Cuts at: 0 1203 1464
Size: 1203 261
EaeI y'GGCC_r
Cuts at: 0 53 377 401 709 1352 1456 1464
Size: 53 324 24 308 643 104 8
Fragments arranged by size:
643 324 308 104 53 24 8
EagI C'GGCC_G
Cuts at: 0 1456 1464
Size: 1456 8
EarI CTCTTCn'nnn_
Cuts at: 0 835 1464
Size: 835 629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 216 1254 1462 1464
Size: 175 41 1038 208 2
Fragments arranged by size:
1038 208 175 41 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1300 1464
Size: 1300 164
EcoO109I rG'GnC_Cy
Cuts at: 0 767 1347 1464
Size: 767 580 117
Fragments arranged by size:
767 580 117
HaeII r_GCGC'y
Cuts at: 0 1232 1464
Size: 1232 232
HincII GTy'rAC
Cuts at: 0 1224 1464
Size: 1224 240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1464
Size: 782 682
MscI TGG'CCA
Cuts at: 0 379 1354 1464
Size: 379 975 110
Fragments arranged by size:
975 379 110
MslI CAynn'nnrTG
Cuts at: 0 94 541 1180 1297 1375 1464
Size: 94 447 639 117 78 89
Fragments arranged by size:
639 447 117 94 89 78
MspA1I CmG'CkG
Cuts at: 0 241 777 809 1143 1464
Size: 241 536 32 334 321
Fragments arranged by size:
536 334 321 241 32
NcoI C'CATG_G
Cuts at: 0 371 1464
Size: 371 1093
NspI r_CATG'y
Cuts at: 0 1278 1464
Size: 1278 186
PasI CC'CwG_GG
Cuts at: 0 840 1312 1464
Size: 840 472 152
Fragments arranged by size:
840 472 152
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 1464
Size: 739 32 693
Fragments arranged by size:
739 693 32
PpuMI rG'GwC_Cy
Cuts at: 0 767 1347 1464
Size: 767 580 117
Fragments arranged by size:
767 580 117
PshAI GACnn'nnGTC
Cuts at: 0 1162 1464
Size: 1162 302
PspOMI G'GGCC_C
Cuts at: 0 506 1464
Size: 506 958
PstI C_TGCA'G
Cuts at: 0 119 823 1424 1464
Size: 119 704 601 40
Fragments arranged by size:
704 601 119 40
PvuII CAG'CTG
Cuts at: 0 1143 1464
Size: 1143 321
SacII CC_GC'GG
Cuts at: 0 242 810 1464
Size: 242 568 654
Fragments arranged by size:
654 568 242
SapI GCTCTTCn'nnn_
Cuts at: 0 835 1464
Size: 835 629
SbfI CC_TGCA'GG
Cuts at: 0 119 1464
Size: 119 1345
SexAI A'CCwGG_T
Cuts at: 0 71 1464
Size: 71 1393
SfcI C'TryA_G
Cuts at: 0 115 464 614 819 1329 1420 1464
Size: 115 349 150 205 510 91 44
Fragments arranged by size:
510 349 205 150 115 91 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 376 484 1464
Size: 376 108 980
Fragments arranged by size:
980 376 108
SmlI C'TyrA_G
Cuts at: 0 172 1464
Size: 172 1292
SphI G_CATG'C
Cuts at: 0 1278 1464
Size: 1278 186
StyI C'CwwG_G
Cuts at: 0 371 593 1464
Size: 371 222 871
Fragments arranged by size:
871 371 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 384 1464
Size: 384 1080
TatI w'GTAC_w
Cuts at: 0 1019 1464
Size: 1019 445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 302 334 1040 1072 1331 1359 1363 1391
Size: 302 32 706 32 259 28 4 28
Cuts at: 1391 1464
Size: 73
Fragments arranged by size:
706 302 259 73 32 32 28 28
4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 1464
Size: 69 1395
XmnI GAAnn'nnTTC
Cuts at: 0 609 1464
Size: 609 855
ZraI GAC'GTC
Cuts at: 0 1164 1464
Size: 1164 300
Enzymes that do cut:
AarI AatII AccI AleI AloI AlwNI ApaI AvaI
BamHI BanI BanII BglI Bme1580I BmgBI BmrI BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI
BstAPI BstXI BstYI BtgI BtsI DraIII DrdI EaeI
EagI EarI Eco57MI EcoNI EcoO109I HaeII HincII MmeI
MscI MslI MspA1I NcoI NspI PasI PpiI PpuMI
PshAI PspOMI PstI PvuII SacII SapI SbfI SexAI
SfcI SfiI SmlI SphI StyI TaqII TatI TstI
XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI Bpu10I BsaBI BsaWI BsiHKAI BsiWI
BspEI BspHI BsrBI BsrFI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI ScaI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI