Restriction map for
pF1KE0341
(Linear) (Six-base) MAPSORT of: KIEE0341.seq Check: 4841 from: 1 to: 2052
KIEE0341.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 0 1529 2052
Size: 1529 523
Acc65I G'GTAC_C
Cuts at: 0 1853 2052
Size: 1853 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 886 2052
Size: 315 571 1166
Fragments arranged by size:
1166 571 315
AfeI AGC'GCT
Cuts at: 0 71 2052
Size: 71 1981
AgeI A'CCGG_T
Cuts at: 0 1541 2052
Size: 1541 511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1815 1936 2052
Size: 1815 121 116
Fragments arranged by size:
1815 121 116
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1621 1653 2052
Size: 1621 32 399
Fragments arranged by size:
1621 399 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1428 2052
Size: 1428 624
ApaI G_GGCC'C
Cuts at: 0 119 186 2052
Size: 119 67 1866
Fragments arranged by size:
1866 119 67
ApoI r'AATT_y
Cuts at: 0 901 1383 1503 2052
Size: 901 482 120 549
Fragments arranged by size:
901 549 482 120
AvaI C'yCGr_G
Cuts at: 0 36 734 835 1065 2052
Size: 36 698 101 230 987
Fragments arranged by size:
987 698 230 101 36
BanI G'GyrC_C
Cuts at: 0 78 221 1853 1946 2052
Size: 78 143 1632 93 106
Fragments arranged by size:
1632 143 106 93 78
BanII G_rGCy'C
Cuts at: 0 119 186 413 1280 1337 1475 2051 2052
Size: 119 67 227 867 57 138 576 1
Fragments arranged by size:
867 576 227 138 119 67 57 1
BbeI G_GCGC'C
Cuts at: 0 1950 2052
Size: 1950 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 1521 1700 2052
Size: 1521 179 352
Fragments arranged by size:
1521 352 179
BciVI GTATCCnnnnn_n'
Cuts at: 0 795 2052
Size: 795 1257
BfrBI ATG'CAT
Cuts at: 0 1075 2052
Size: 1075 977
BlpI GC'TnA_GC
Cuts at: 0 307 724 2052
Size: 307 417 1328
Fragments arranged by size:
1328 417 307
Bme1580I G_kGCm'C
Cuts at: 0 83 119 186 992 1556 1730 2052
Size: 83 36 67 806 564 174 322
Fragments arranged by size:
806 564 322 174 83 67 36
BmgBI CAC'GTC
Cuts at: 0 444 2052
Size: 444 1608
BmtI G_CTAG'C
Cuts at: 0 1109 2052
Size: 1109 943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 1426 1459 1774 1972 1991 2052
Size: 1258 168 33 315 198 19 61
Fragments arranged by size:
1258 315 198 168 61 33 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 2052
Size: 799 1253
BsaI GGTCTCn'nnnn_
Cuts at: 0 764 2052
Size: 764 1288
BsaHI Gr'CG_yC
Cuts at: 0 362 1526 1947 2052
Size: 362 1164 421 105
Fragments arranged by size:
1164 421 362 105
BsaWI w'CCGG_w
Cuts at: 0 1172 1541 2052
Size: 1172 369 511
Fragments arranged by size:
1172 511 369
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1621 1651 2052
Size: 1621 30 401
Fragments arranged by size:
1621 401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1423 2052
Size: 1423 629
BseYI C'CCAG_C
Cuts at: 0 66 105 112 405 1183 1433 2052
Size: 66 39 7 293 778 250 619
Fragments arranged by size:
778 619 293 250 66 39 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 543 1631 2052
Size: 46 497 1088 421
Fragments arranged by size:
1088 497 421 46
BsiEI CG_ry'CG
Cuts at: 0 1724 2052
Size: 1724 328
BsiHKAI G_wGCw'C
Cuts at: 0 1244 1280 2051 2052
Size: 1244 36 771 1
Fragments arranged by size:
1244 771 36 1
BsmI GAATG_Cn'
Cuts at: 0 1075 2052
Size: 1075 977
Bsp1286I G_dGCh'C
Cuts at: 0 83 119 186 413 992 1244 1280 1337
Size: 83 36 67 227 579 252 36 57
Cuts at: 1337 1475 1556 1730 2051 2052
Size: 138 81 174 321 1
Fragments arranged by size:
579 321 252 227 174 138 83 81
67 57 36 36 1
BsrBI CCG'CTC
Cuts at: 0 180 731 2052
Size: 180 551 1321
Fragments arranged by size:
1321 551 180
BsrFI r'CCGG_y
Cuts at: 0 1229 1541 2052
Size: 1229 312 511
Fragments arranged by size:
1229 511 312
BssHII G'CGCG_C
Cuts at: 0 150 2052
Size: 150 1902
BssSI C'ACGA_G
Cuts at: 0 1873 2052
Size: 1873 179
BstXI CCAn_nnnn'nTGG
Cuts at: 0 766 1972 2052
Size: 766 1206 80
Fragments arranged by size:
1206 766 80
BstYI r'GATC_y
Cuts at: 0 852 2052
Size: 852 1200
Bsu36I CC'TnA_GG
Cuts at: 0 623 1858 2052
Size: 623 1235 194
Fragments arranged by size:
1235 623 194
BtgI C'CryG_G
Cuts at: 0 119 187 217 614 1197 1388 1670 2052
Size: 119 68 30 397 583 191 282 382
Fragments arranged by size:
583 397 382 282 191 119 68 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 420 2052
Size: 39 381 1632
Fragments arranged by size:
1632 381 39
BtsI GCAGTG_nn'
Cuts at: 0 176 2052
Size: 176 1876
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1377 1412 2052
Size: 1377 35 640
Fragments arranged by size:
1377 640 35
DraIII CAC_nnn'GTG
Cuts at: 0 1164 1689 1905 2052
Size: 1164 525 216 147
Fragments arranged by size:
1164 525 216 147
DrdI GACnn_nn'nnGTC
Cuts at: 0 1525 2052
Size: 1525 527
EaeI y'GGCC_r
Cuts at: 0 50 477 857 1358 1673 2052
Size: 50 427 380 501 315 379
Fragments arranged by size:
501 427 380 379 315 50
EarI CTCTTCn'nnn_
Cuts at: 0 334 1480 2052
Size: 334 1146 572
Fragments arranged by size:
1146 572 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 274 1630 2052
Size: 274 1356 422
Fragments arranged by size:
1356 422 274
EcoICRI GAG'CTC
Cuts at: 0 1278 2049 2052
Size: 1278 771 3
Fragments arranged by size:
1278 771 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 886 1258 1426 1459 1774 1972 1991
Size: 315 571 372 168 33 315 198 19
Cuts at: 1991 2052
Size: 61
Fragments arranged by size:
571 372 315 315 198 168 61 33
19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 955 1375 2052
Size: 955 420 677
Fragments arranged by size:
955 677 420
EcoO109I rG'GnC_Cy
Cuts at: 0 31 183 2011 2052
Size: 31 152 1828 41
Fragments arranged by size:
1828 152 41 31
EcoRI G'AATT_C
Cuts at: 0 1383 2052
Size: 1383 669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1545 2052
Size: 1545 507
HaeII r_GCGC'y
Cuts at: 0 73 113 1749 1950 2052
Size: 73 40 1636 201 102
Fragments arranged by size:
1636 201 102 73 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 595 627 642 674 1528 1560
Size: 283 32 280 32 15 32 854 32
Cuts at: 1560 2052
Size: 492
Fragments arranged by size:
854 492 283 280 32 32 32 32
15
HincII GTy'rAC
Cuts at: 0 570 2052
Size: 570 1482
HindIII A'AGCT_T
Cuts at: 0 1782 2052
Size: 1782 270
KasI G'GCGC_C
Cuts at: 0 1946 2052
Size: 1946 106
KpnI G_GTAC'C
Cuts at: 0 1857 2052
Size: 1857 195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 2052
Size: 747 1305
MscI TGG'CCA
Cuts at: 0 52 479 859 2052
Size: 52 427 380 1193
Fragments arranged by size:
1193 427 380 52
MslI CAynn'nnrTG
Cuts at: 0 985 1423 1486 2052
Size: 985 438 63 566
Fragments arranged by size:
985 566 438 63
MspA1I CmG'CkG
Cuts at: 0 13 121 189 219 1010 1095 1293 1437
Size: 13 108 68 30 791 85 198 144
Cuts at: 1437 2052
Size: 615
Fragments arranged by size:
791 615 198 144 108 85 68 30
13
NarI GG'CG_CC
Cuts at: 0 1947 2052
Size: 1947 105
NdeI CA'TA_TG
Cuts at: 0 719 2052
Size: 719 1333
NheI G'CTAG_C
Cuts at: 0 1105 2052
Size: 1105 947
NsiI A_TGCA'T
Cuts at: 0 1077 2052
Size: 1077 975
PflMI CCAn_nnn'nTGG
Cuts at: 0 1164 2052
Size: 1164 888
PfoI T'CCnGG_A
Cuts at: 0 908 1235 1751 2052
Size: 908 327 516 301
Fragments arranged by size:
908 516 327 301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1621 1653 2052
Size: 1621 32 399
Fragments arranged by size:
1621 399 32
PpuMI rG'GwC_Cy
Cuts at: 0 31 2052
Size: 31 2021
PspOMI G'GGCC_C
Cuts at: 0 115 182 2052
Size: 115 67 1870
Fragments arranged by size:
1870 115 67
PspXI vC'TCGA_Gb
Cuts at: 0 835 2052
Size: 835 1217
PstI C_TGCA'G
Cuts at: 0 131 149 2052
Size: 131 18 1903
Fragments arranged by size:
1903 131 18
PvuI CG_AT'CG
Cuts at: 0 1724 2052
Size: 1724 328
PvuII CAG'CTG
Cuts at: 0 1293 1437 2052
Size: 1293 144 615
Fragments arranged by size:
1293 615 144
SacI G_AGCT'C
Cuts at: 0 1280 2051 2052
Size: 1280 771 1
Fragments arranged by size:
1280 771 1
SacII CC_GC'GG
Cuts at: 0 122 190 220 2052
Size: 122 68 30 1832
Fragments arranged by size:
1832 122 68 30
SanDI GG'GwC_CC
Cuts at: 0 31 2052
Size: 31 2021
SapI GCTCTTCn'nnn_
Cuts at: 0 334 1480 2052
Size: 334 1146 572
Fragments arranged by size:
1146 572 334
SexAI A'CCwGG_T
Cuts at: 0 563 2052
Size: 563 1489
SfcI C'TryA_G
Cuts at: 0 127 145 1605 2052
Size: 127 18 1460 447
Fragments arranged by size:
1460 447 127 18
SfoI GGC'GCC
Cuts at: 0 1948 2052
Size: 1948 104
SmaI CCC'GGG
Cuts at: 0 38 736 2052
Size: 38 698 1316
Fragments arranged by size:
1316 698 38
SmlI C'TyrA_G
Cuts at: 0 814 835 2052
Size: 814 21 1217
Fragments arranged by size:
1217 814 21
StuI AGG'CCT
Cuts at: 0 469 2052
Size: 469 1583
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 376 476 688 772 2052
Size: 80 296 100 212 84 1280
Fragments arranged by size:
1280 296 212 100 84 80
TatI w'GTAC_w
Cuts at: 0 422 2052
Size: 422 1630
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1161 1425 2052
Size: 1161 264 627
Fragments arranged by size:
1161 627 264
XhoI C'TCGA_G
Cuts at: 0 835 2052
Size: 835 1217
XmaI C'CCGG_G
Cuts at: 0 36 734 2052
Size: 36 698 1318
Fragments arranged by size:
1318 698 36
XmnI GAAnn'nnTTC
Cuts at: 0 904 2052
Size: 904 1148
ZraI GAC'GTC
Cuts at: 0 1527 2052
Size: 1527 525
Enzymes that do cut:
AatII Acc65I AcuI AfeI AgeI AlfI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BciVI
BfrBI BlpI Bme1580I BmgBI BmtI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BsrBI BsrFI BssHII BssSI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NarI NdeI NheI NsiI PflMI PfoI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII SacI
SacII SanDI SapI SexAI SfcI SfoI SmaI SmlI
StuI TaqII TatI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AflIII AhdI AleI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BclI BglI BglII BmrI BplI Bpu10I BsaAI BsaBI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI BstAPI
BstBI BstEII BstZ17I ClaI DraI EagI EcoRV FseI
FspI FspAI HpaI MfeI MluI NaeI NcoI NgoMIV
NotI NruI NspI PacI PasI PciI PmeI PmlI
PshAI PsiI PsrI RsrII SalI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI
SwaI TstI Tth111I XbaI