Restriction map for
pF1KE0350
(Linear) (Six-base) MAPSORT of: KIEE0350.seq Check: 9277 from: 1 to: 2241
KIEE0350.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 1939 2241
Size: 1939 302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 101 811 1897 2241
Size: 44 57 710 1086 344
Fragments arranged by size:
1086 710 344 57 44
AflIII A'CryG_T
Cuts at: 0 1562 1640 2241
Size: 1562 78 601
Fragments arranged by size:
1562 601 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 939 2241
Size: 939 1302
ApoI r'AATT_y
Cuts at: 0 732 1407 1788 1835 2072 2241
Size: 732 675 381 47 237 169
Fragments arranged by size:
732 675 381 237 169 47
AseI AT'TA_AT
Cuts at: 0 1193 2241
Size: 1193 1048
BanI G'GyrC_C
Cuts at: 0 785 2241
Size: 785 1456
BanII G_rGCy'C
Cuts at: 0 1271 1734 1979 2241
Size: 1271 463 245 262
Fragments arranged by size:
1271 463 262 245
BbeI G_GCGC'C
Cuts at: 0 789 2241
Size: 789 1452
BbsI GAAGACnn'nnnn_
Cuts at: 0 244 1183 1494 2004 2241
Size: 244 939 311 510 237
Fragments arranged by size:
939 510 311 244 237
BbvCI CC'TCA_GC
Cuts at: 0 2219 2241
Size: 2219 22
BfrBI ATG'CAT
Cuts at: 0 434 1050 2241
Size: 434 616 1191
Fragments arranged by size:
1191 616 434
BglII A'GATC_T
Cuts at: 0 134 2241
Size: 134 2107
BlpI GC'TnA_GC
Cuts at: 0 1265 2020 2241
Size: 1265 755 221
Fragments arranged by size:
1265 755 221
Bme1580I G_kGCm'C
Cuts at: 0 429 2241
Size: 429 1812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1456 1748 2241
Size: 202 1254 292 493
Fragments arranged by size:
1254 493 292 202
Bpu10I CC'TnA_GC
Cuts at: 0 686 2219 2241
Size: 686 1533 22
Fragments arranged by size:
1533 686 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1492 2137 2241
Size: 740 752 645 104
Fragments arranged by size:
752 740 645 104
BsaAI yAC'GTr
Cuts at: 0 439 2241
Size: 439 1802
BsaHI Gr'CG_yC
Cuts at: 0 786 1095 2241
Size: 786 309 1146
Fragments arranged by size:
1146 786 309
BsaWI w'CCGG_w
Cuts at: 0 2144 2241
Size: 2144 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 1237 1267 1552 1582 2241
Size: 175 30 1032 30 285 30 659
Fragments arranged by size:
1032 659 285 175 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 545 792 1308 1560 2098 2241
Size: 545 247 516 252 538 143
Fragments arranged by size:
545 538 516 252 247 143
BseYI C'CCAG_C
Cuts at: 0 18 2241
Size: 18 2223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1988 2241
Size: 373 1615 253
Fragments arranged by size:
1615 373 253
BsiHKAI G_wGCw'C
Cuts at: 0 1664 2026 2241
Size: 1664 362 215
Fragments arranged by size:
1664 362 215
BsmI GAATG_Cn'
Cuts at: 0 1753 2241
Size: 1753 488
Bsp1286I G_dGCh'C
Cuts at: 0 429 1271 1664 1734 1979 2026 2241
Size: 429 842 393 70 245 47 215
Fragments arranged by size:
842 429 393 245 215 70 47
BspEI T'CCGG_A
Cuts at: 0 2144 2241
Size: 2144 97
BspHI T'CATG_A
Cuts at: 0 153 1745 1757 2079 2241
Size: 153 1592 12 322 162
Fragments arranged by size:
1592 322 162 153 12
BsrDI GCAATG_nn'
Cuts at: 0 53 2241
Size: 53 2188
BsrFI r'CCGG_y
Cuts at: 0 782 2241
Size: 782 1459
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1346 2241
Size: 1346 895
BstYI r'GATC_y
Cuts at: 0 134 1382 2241
Size: 134 1248 859
Fragments arranged by size:
1248 859 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1827 2241
Size: 1827 414
BtsI GCAGTG_nn'
Cuts at: 0 1344 2071 2241
Size: 1344 727 170
Fragments arranged by size:
1344 727 170
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1536 1571 2241
Size: 1536 35 670
Fragments arranged by size:
1536 670 35
DraI TTT'AAA
Cuts at: 0 1793 2241
Size: 1793 448
DraIII CAC_nnn'GTG
Cuts at: 0 265 2241
Size: 265 1976
DrdI GACnn_nn'nnGTC
Cuts at: 0 988 2241
Size: 988 1253
EaeI y'GGCC_r
Cuts at: 0 1287 1557 2241
Size: 1287 270 684
Fragments arranged by size:
1287 684 270
EarI CTCTTCn'nnn_
Cuts at: 0 20 2241
Size: 20 2221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 101 202 811 1456 1748 1897 2241
Size: 44 57 101 609 645 292 149 344
Fragments arranged by size:
645 609 344 292 149 101 57 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1243 1675 2241
Size: 1243 432 566
Fragments arranged by size:
1243 566 432
EcoO109I rG'GnC_Cy
Cuts at: 0 576 760 1164 2125 2241
Size: 576 184 404 961 116
Fragments arranged by size:
961 576 404 184 116
EcoRV GAT'ATC
Cuts at: 0 1773 2142 2241
Size: 1773 369 99
Fragments arranged by size:
1773 369 99
FspI TGC'GCA
Cuts at: 0 52 2241
Size: 52 2189
HaeII r_GCGC'y
Cuts at: 0 789 2241
Size: 789 1452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 175 207 358 390 525 557
Size: 135 32 8 32 151 32 135 32
Cuts at: 557 1528 1560 1759 1791 2241
Size: 971 32 199 32 450
Fragments arranged by size:
971 450 199 151 135 135 32 32
32 32 32 32 8
KasI G'GCGC_C
Cuts at: 0 785 2241
Size: 785 1456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1731 2241
Size: 343 1388 510
Fragments arranged by size:
1388 510 343
MscI TGG'CCA
Cuts at: 0 1559 2241
Size: 1559 682
MslI CAynn'nnrTG
Cuts at: 0 296 911 1055 1453 1657 1846 2241
Size: 296 615 144 398 204 189 395
Fragments arranged by size:
615 398 395 296 204 189 144
MspA1I CmG'CkG
Cuts at: 0 22 2241
Size: 22 2219
NarI GG'CG_CC
Cuts at: 0 786 2241
Size: 786 1455
NsiI A_TGCA'T
Cuts at: 0 436 1052 2241
Size: 436 616 1189
Fragments arranged by size:
1189 616 436
NspI r_CATG'y
Cuts at: 0 453 1264 1566 1644 2241
Size: 453 811 302 78 597
Fragments arranged by size:
811 597 453 302 78
PciI A'CATG_T
Cuts at: 0 1562 1640 2241
Size: 1562 78 601
Fragments arranged by size:
1562 601 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 1423 2241
Size: 1423 818
PpuMI rG'GwC_Cy
Cuts at: 0 576 760 1164 2241
Size: 576 184 404 1077
Fragments arranged by size:
1077 576 404 184
PsiI TTA'TAA
Cuts at: 0 884 2241
Size: 884 1357
PstI C_TGCA'G
Cuts at: 0 892 2241
Size: 892 1349
PvuII CAG'CTG
Cuts at: 0 22 2241
Size: 22 2219
ScaI AGT'ACT
Cuts at: 0 1816 2241
Size: 1816 425
SexAI A'CCwGG_T
Cuts at: 0 1238 2191 2241
Size: 1238 953 50
Fragments arranged by size:
1238 953 50
SfcI C'TryA_G
Cuts at: 0 888 993 1142 1810 1940 2241
Size: 888 105 149 668 130 301
Fragments arranged by size:
888 668 301 149 130 105
SfoI GGC'GCC
Cuts at: 0 787 2241
Size: 787 1454
SgrAI Cr'CCGG_yG
Cuts at: 0 782 2241
Size: 782 1459
SmlI C'TyrA_G
Cuts at: 0 755 1507 2116 2241
Size: 755 752 609 125
Fragments arranged by size:
755 752 609 125
SphI G_CATG'C
Cuts at: 0 1264 2241
Size: 1264 977
SspI AAT'ATT
Cuts at: 0 1204 1443 2241
Size: 1204 239 798
Fragments arranged by size:
1204 798 239
StyI C'CwwG_G
Cuts at: 0 228 1970 2241
Size: 228 1742 271
Fragments arranged by size:
1742 271 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 919 1753 2241
Size: 919 834 488
Fragments arranged by size:
919 834 488
TatI w'GTAC_w
Cuts at: 0 1814 2241
Size: 1814 427
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1552 1584 2241
Size: 158 32 1362 32 657
Fragments arranged by size:
1362 657 158 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 189 2241
Size: 189 2052
XbaI T'CTAG_A
Cuts at: 0 1878 2241
Size: 1878 363
XmnI GAAnn'nnTTC
Cuts at: 0 1444 2241
Size: 1444 797
Enzymes that do cut:
AccI AcuI AflIII AlfI ApoI AseI BanI BanII
BbeI BbsI BbvCI BfrBI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI
BstAPI BstYI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NsiI
NspI PciI PflMI PpuMI PsiI PstI PvuII ScaI
SexAI SfcI SfoI SgrAI SmlI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BcgI BciVI BclI BglI BmgBI
BmrI BmtI BplI BsaI BsaBI BsiEI BsiWI BsmBI
BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI ClaI EagI EciI EcoICRI EcoRI
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PfoI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SgfI SmaI SnaBI SpeI SrfI
StuI SwaI XcmI XhoI XmaI ZraI