Restriction map for
pF1KE0351
(Linear) (Six-base) MAPSORT of: KIEE0351.seq Check: 7487 from: 1 to: 2454
KIEE0351.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 885 2296 2454
Size: 885 1411 158
Fragments arranged by size:
1411 885 158
AccI GT'mk_AC
Cuts at: 0 154 2454
Size: 154 2300
Acc65I G'GTAC_C
Cuts at: 0 713 957 2454
Size: 713 244 1497
Fragments arranged by size:
1497 713 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 934 1053 1492 2431 2454
Size: 586 348 119 439 939 23
Fragments arranged by size:
939 586 439 348 119 23
AleI CACnn'nnGTG
Cuts at: 0 523 2454
Size: 523 1931
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 834 2454
Size: 834 1620
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 2454
Size: 394 32 2028
Fragments arranged by size:
2028 394 32
AlwNI CAG_nnn'CTG
Cuts at: 0 613 924 1041 1332 2397 2454
Size: 613 311 117 291 1065 57
Fragments arranged by size:
1065 613 311 291 117 57
ApaI G_GGCC'C
Cuts at: 0 2076 2454
Size: 2076 378
ApaLI G'TGCA_C
Cuts at: 0 838 2454
Size: 838 1616
AvaI C'yCGr_G
Cuts at: 0 473 995 1163 1701 2075 2093 2164 2454
Size: 473 522 168 538 374 18 71 290
Fragments arranged by size:
538 522 473 374 290 168 71 18
BamHI G'GATC_C
Cuts at: 0 1541 2454
Size: 1541 913
BanI G'GyrC_C
Cuts at: 0 662 713 957 991 2380 2454
Size: 662 51 244 34 1389 74
Fragments arranged by size:
1389 662 244 74 51 34
BanII G_rGCy'C
Cuts at: 0 676 1502 1553 1691 1922 2076 2255 2454
Size: 676 826 51 138 231 154 179 199
Fragments arranged by size:
826 676 231 199 179 154 138 51
BbeI G_GCGC'C
Cuts at: 0 666 2454
Size: 666 1788
BbsI GAAGACnn'nnnn_
Cuts at: 0 2333 2454
Size: 2333 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2163 2197 2454
Size: 2163 34 257
Fragments arranged by size:
2163 257 34
BglI GCCn_nnn'nGGC
Cuts at: 0 732 2454
Size: 732 1722
BlpI GC'TnA_GC
Cuts at: 0 268 655 1247 2454
Size: 268 387 592 1207
Fragments arranged by size:
1207 592 387 268
Bme1580I G_kGCm'C
Cuts at: 0 607 728 842 996 2076 2448 2454
Size: 607 121 114 154 1080 372 6
Fragments arranged by size:
1080 607 372 154 121 114 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 283 568 574 660 1162 1210 1255
Size: 106 177 285 6 86 502 48 45
Cuts at: 1255 1315 1435 1599 1741 1801 1810 2239 2454
Size: 60 120 164 142 60 9 429 215
Fragments arranged by size:
502 429 285 215 177 164 142 120
106 86 60 60 48 45 9 6
Bpu10I CC'TnA_GC
Cuts at: 0 880 1496 2454
Size: 880 616 958
Fragments arranged by size:
958 880 616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1407 2454
Size: 790 617 1047
Fragments arranged by size:
1047 790 617
BsaI GGTCTCn'nnnn_
Cuts at: 0 932 2454
Size: 932 1522
BsaAI yAC'GTr
Cuts at: 0 138 1091 2454
Size: 138 953 1363
Fragments arranged by size:
1363 953 138
BsaBI GATnn'nnATC
Cuts at: 0 497 2454
Size: 497 1957
BsaHI Gr'CG_yC
Cuts at: 0 663 1940 2454
Size: 663 1277 514
Fragments arranged by size:
1277 663 514
BsaWI w'CCGG_w
Cuts at: 0 359 2417 2454
Size: 359 2058 37
Fragments arranged by size:
2058 359 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 1576 1606 1714 1744 1950 1980 2454
Size: 25 1551 30 108 30 206 30 474
Fragments arranged by size:
1551 474 206 108 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 409 684 841 880 1001 1551 1701
Size: 406 3 275 157 39 121 550 150
Cuts at: 1701 2454
Size: 753
Fragments arranged by size:
753 550 406 275 157 150 121 39
3
BseYI C'CCAG_C
Cuts at: 0 104 709 1041 1897 2104 2454
Size: 104 605 332 856 207 350
Fragments arranged by size:
856 605 350 332 207 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1291 1816 2167 2386 2454
Size: 881 410 525 351 219 68
Fragments arranged by size:
881 525 410 351 219 68
BsiEI CG_ry'CG
Cuts at: 0 158 2454
Size: 158 2296
BsiHKAI G_wGCw'C
Cuts at: 0 520 676 842 1172 1502 2255 2454
Size: 520 156 166 330 330 753 199
Fragments arranged by size:
753 520 330 330 199 166 156
BsmI GAATG_Cn'
Cuts at: 0 2140 2454
Size: 2140 314
Bsp1286I G_dGCh'C
Cuts at: 0 520 607 676 728 842 996 1172 1502
Size: 520 87 69 52 114 154 176 330
Cuts at: 1502 1553 1691 1922 2076 2255 2448 2454
Size: 51 138 231 154 179 193 6
Fragments arranged by size:
520 330 231 193 179 176 154 154
138 114 87 69 52 51 6
BspEI T'CCGG_A
Cuts at: 0 359 2454
Size: 359 2095
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 107 885 950 1610 2296 2454
Size: 67 40 778 65 660 686 158
Fragments arranged by size:
778 686 660 158 67 65 40
BsrBI CCG'CTC
Cuts at: 0 659 2454
Size: 659 1795
BsrDI GCAATG_nn'
Cuts at: 0 690 2454
Size: 690 1764
BssSI C'ACGA_G
Cuts at: 0 1728 2454
Size: 1728 726
BstAPI GCAn_nnn'nTGC
Cuts at: 0 924 2454
Size: 924 1530
BstYI r'GATC_y
Cuts at: 0 1541 1556 2271 2454
Size: 1541 15 715 183
Fragments arranged by size:
1541 715 183 15
Bsu36I CC'TnA_GG
Cuts at: 0 667 730 824 2111 2398 2454
Size: 667 63 94 1287 287 56
Fragments arranged by size:
1287 667 287 94 63 56
BtsI GCAGTG_nn'
Cuts at: 0 2044 2454
Size: 2044 410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1251 1286 2454
Size: 1251 35 1168
Fragments arranged by size:
1251 1168 35
DraI TTT'AAA
Cuts at: 0 1767 2454
Size: 1767 687
EaeI y'GGCC_r
Cuts at: 0 30 833 902 977 1017 1175 1311 1981
Size: 30 803 69 75 40 158 136 670
Cuts at: 1981 2262 2454
Size: 281 192
Fragments arranged by size:
803 670 281 192 158 136 75 69
40 30
EarI CTCTTCn'nnn_
Cuts at: 0 1507 2149 2454
Size: 1507 642 305
Fragments arranged by size:
1507 642 305
EcoICRI GAG'CTC
Cuts at: 0 674 1500 2253 2454
Size: 674 826 753 201
Fragments arranged by size:
826 753 674 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 283 568 574 586 660 934 1053
Size: 106 177 285 6 12 74 274 119
Cuts at: 1053 1162 1210 1255 1315 1435 1492 1599 1741
Size: 109 48 45 60 120 57 107 142
Cuts at: 1741 1801 1810 2239 2431 2454
Size: 60 9 429 192 23
Fragments arranged by size:
429 285 274 192 177 142 120 119
109 107 106 74 60 60 57 48
45 23 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 916 946 2454
Size: 916 30 1508
Fragments arranged by size:
1508 916 30
EcoO109I rG'GnC_Cy
Cuts at: 0 81 572 734 909 921 1116 1137 1147
Size: 81 491 162 175 12 195 21 10
Cuts at: 1147 1481 1706 1787 1821 2169 2394 2454
Size: 334 225 81 34 348 225 60
Fragments arranged by size:
491 348 334 225 225 195 175 162
81 81 60 34 21 12 10
HaeII r_GCGC'y
Cuts at: 0 666 1212 1288 1753 2340 2454
Size: 666 546 76 465 587 114
Fragments arranged by size:
666 587 546 465 114 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 271 303 2454
Size: 27 244 32 2151
Fragments arranged by size:
2151 244 32 27
HincII GTy'rAC
Cuts at: 0 155 2454
Size: 155 2299
KasI G'GCGC_C
Cuts at: 0 662 2454
Size: 662 1792
KpnI G_GTAC'C
Cuts at: 0 717 961 2454
Size: 717 244 1493
Fragments arranged by size:
1493 717 244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2330 2454
Size: 2330 124
MscI TGG'CCA
Cuts at: 0 32 904 979 1177 2264 2454
Size: 32 872 75 198 1087 190
Fragments arranged by size:
1087 872 198 190 75 32
MslI CAynn'nnrTG
Cuts at: 0 523 2454
Size: 523 1931
MspA1I CmG'CkG
Cuts at: 0 150 523 709 930 1830 1905 2193 2454
Size: 150 373 186 221 900 75 288 261
Fragments arranged by size:
900 373 288 261 221 186 150 75
NarI GG'CG_CC
Cuts at: 0 663 2454
Size: 663 1791
PasI CC'CwG_GG
Cuts at: 0 384 2454
Size: 384 2070
PflMI CCAn_nnn'nTGG
Cuts at: 0 633 1026 1320 2454
Size: 633 393 294 1134
Fragments arranged by size:
1134 633 393 294
PfoI T'CCnGG_A
Cuts at: 0 83 1544 1642 2454
Size: 83 1461 98 812
Fragments arranged by size:
1461 812 98 83
PpuMI rG'GwC_Cy
Cuts at: 0 81 572 1116 1137 1147 1706 2454
Size: 81 491 544 21 10 559 748
Fragments arranged by size:
748 559 544 491 81 21 10
PshAI GACnn'nnGTC
Cuts at: 0 200 2454
Size: 200 2254
PspOMI G'GGCC_C
Cuts at: 0 2072 2454
Size: 2072 382
PstI C_TGCA'G
Cuts at: 0 803 929 947 1739 2051 2393 2454
Size: 803 126 18 792 312 342 61
Fragments arranged by size:
803 792 342 312 126 61 18
PvuII CAG'CTG
Cuts at: 0 150 709 930 1830 1905 2454
Size: 150 559 221 900 75 549
Fragments arranged by size:
900 559 549 221 150 75
SacI G_AGCT'C
Cuts at: 0 676 1502 2255 2454
Size: 676 826 753 199
Fragments arranged by size:
826 753 676 199
SalI G'TCGA_C
Cuts at: 0 153 2454
Size: 153 2301
SanDI GG'GwC_CC
Cuts at: 0 1116 2454
Size: 1116 1338
SapI GCTCTTCn'nnn_
Cuts at: 0 1507 2454
Size: 1507 947
SbfI CC_TGCA'GG
Cuts at: 0 947 2393 2454
Size: 947 1446 61
Fragments arranged by size:
1446 947 61
SfcI C'TryA_G
Cuts at: 0 145 799 925 943 1735 1845 2047 2389
Size: 145 654 126 18 792 110 202 342
Cuts at: 2389 2454
Size: 65
Fragments arranged by size:
792 654 342 202 145 126 110 65
18
SfoI GGC'GCC
Cuts at: 0 664 2454
Size: 664 1790
SmaI CCC'GGG
Cuts at: 0 997 1165 2077 2095 2454
Size: 997 168 912 18 359
Fragments arranged by size:
997 912 359 168 18
SmlI C'TyrA_G
Cuts at: 0 805 1386 2454
Size: 805 581 1068
Fragments arranged by size:
1068 805 581
SrfI GCCC'GGGC
Cuts at: 0 997 2454
Size: 997 1457
StuI AGG'CCT
Cuts at: 0 52 223 874 1726 2436 2454
Size: 52 171 651 852 710 18
Fragments arranged by size:
852 710 651 171 52 18
StyI C'CwwG_G
Cuts at: 0 905 1913 2128 2440 2454
Size: 905 1008 215 312 14
Fragments arranged by size:
1008 905 312 215 14
TatI w'GTAC_w
Cuts at: 0 2063 2454
Size: 2063 391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 189 221 1108 1140 1714 1746 1950 1982
Size: 189 32 887 32 574 32 204 32
Cuts at: 1982 2454
Size: 472
Fragments arranged by size:
887 574 472 204 189 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 289 2454
Size: 289 2165
XbaI T'CTAG_A
Cuts at: 0 2424 2454
Size: 2424 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1185 1269 2454
Size: 1185 84 1185
Fragments arranged by size:
1185 1185 84
XmaI C'CCGG_G
Cuts at: 0 995 1163 2075 2093 2454
Size: 995 168 912 18 361
Fragments arranged by size:
995 912 361 168 18
XmnI GAAnn'nnTTC
Cuts at: 0 367 2454
Size: 367 2087
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BcgI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BssSI BstAPI BstYI Bsu36I BtsI CspCI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NarI PasI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SalI SanDI SapI SbfI SfcI SfoI SmaI SmlI
SrfI StuI StyI TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI BsiWI BsmBI
BspHI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I
BtgI BtgZI ClaI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI TaqII
XhoI ZraI