Restriction map for
pF1KE0354
(Linear) (Six-base) MAPSORT of: KIEE0354.seq Check: 1904 from: 1 to: 2157
KIEE0354.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2071 2157
Size: 2071 86
AatII G_ACGT'C
Cuts at: 0 455 2157
Size: 455 1702
AflIII A'CryG_T
Cuts at: 0 287 776 1352 2157
Size: 287 489 576 805
Fragments arranged by size:
805 576 489 287
AhdI GACnn_n'nnGTC
Cuts at: 0 1614 2157
Size: 1614 543
AleI CACnn'nnGTG
Cuts at: 0 286 823 1579 2157
Size: 286 537 756 578
Fragments arranged by size:
756 578 537 286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 943 975 2157
Size: 943 32 1182
Fragments arranged by size:
1182 943 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1674 1787 1800 2091 2157
Size: 1674 113 13 291 66
Fragments arranged by size:
1674 291 113 66 13
ApaI G_GGCC'C
Cuts at: 0 534 1039 1208 1243 1630 1752 2157
Size: 534 505 169 35 387 122 405
Fragments arranged by size:
534 505 405 387 169 122 35
ApaLI G'TGCA_C
Cuts at: 0 1269 2157
Size: 1269 888
BamHI G'GATC_C
Cuts at: 0 128 2157
Size: 128 2029
BanI G'GyrC_C
Cuts at: 0 65 715 1307 1506 2157
Size: 65 650 592 199 651
Fragments arranged by size:
651 650 592 199 65
BanII G_rGCy'C
Cuts at: 0 534 1024 1039 1208 1243 1630 1752 1781
Size: 534 490 15 169 35 387 122 29
Cuts at: 1781 2157
Size: 376
Fragments arranged by size:
534 490 387 376 169 122 35 29
15
BbeI G_GCGC'C
Cuts at: 0 69 719 1311 2157
Size: 69 650 592 846
Fragments arranged by size:
846 650 592 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 405 1548 2157
Size: 275 130 1143 609
Fragments arranged by size:
1143 609 275 130
BbvCI CC'TCA_GC
Cuts at: 0 1447 1933 2157
Size: 1447 486 224
Fragments arranged by size:
1447 486 224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 95 2157
Size: 61 34 2062
Fragments arranged by size:
2062 61 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 359 2157
Size: 359 1798
BclI T'GATC_A
Cuts at: 0 1598 2157
Size: 1598 559
BglII A'GATC_T
Cuts at: 0 1007 2157
Size: 1007 1150
BlpI GC'TnA_GC
Cuts at: 0 248 1018 2157
Size: 248 770 1139
Fragments arranged by size:
1139 770 248
Bme1580I G_kGCm'C
Cuts at: 0 534 796 1039 1208 1236 1243 1273 1630
Size: 534 262 243 169 28 7 30 357
Cuts at: 1630 1752 2157
Size: 122 405
Fragments arranged by size:
534 405 357 262 243 169 122 30
28 7
BmgBI CAC'GTC
Cuts at: 0 288 1065 1464 1596 2157
Size: 288 777 399 132 561
Fragments arranged by size:
777 561 399 288 132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1923 2157
Size: 1923 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 900 1189 1606 1663 1696 1700 1756
Size: 562 338 289 417 57 33 4 56
Cuts at: 1756 1888 2157
Size: 132 269
Fragments arranged by size:
562 417 338 289 269 132 57 56
33 4
Bpu10I CC'TnA_GC
Cuts at: 0 1447 1780 1933 2157
Size: 1447 333 153 224
Fragments arranged by size:
1447 333 224 153
BsaAI yAC'GTr
Cuts at: 0 1353 2157
Size: 1353 804
BsaHI Gr'CG_yC
Cuts at: 0 66 410 452 716 1308 2157
Size: 66 344 42 264 592 849
Fragments arranged by size:
849 592 344 264 66 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 291 321 1636 1648 1666 1678 2157
Size: 291 30 1315 12 18 12 479
Fragments arranged by size:
1315 479 291 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 976 1195 1384 1699 2157
Size: 58 918 219 189 315 458
Fragments arranged by size:
918 458 315 219 189 58
BseYI C'CCAG_C
Cuts at: 0 49 186 899 1032 1039 1085 1217 1242
Size: 49 137 713 133 7 46 132 25
Cuts at: 1242 1377 1647 2157
Size: 135 270 510
Fragments arranged by size:
713 510 270 137 135 133 132 49
46 25 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 2157
Size: 1024 1133
BsiEI CG_ry'CG
Cuts at: 0 1437 1580 2157
Size: 1437 143 577
Fragments arranged by size:
1437 577 143
BsiHKAI G_wGCw'C
Cuts at: 0 644 1273 1516 2157
Size: 644 629 243 641
Fragments arranged by size:
644 641 629 243
BsmI GAATG_Cn'
Cuts at: 0 1953 2157
Size: 1953 204
Bsp1286I G_dGCh'C
Cuts at: 0 534 644 796 1024 1039 1208 1236 1243
Size: 534 110 152 228 15 169 28 7
Cuts at: 1243 1273 1516 1630 1752 1781 2157
Size: 30 243 114 122 29 376
Fragments arranged by size:
534 376 243 228 169 152 122 114
110 30 29 28 15 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1619 1643 2071 2157
Size: 1619 24 428 86
Fragments arranged by size:
1619 428 86 24
BsrBI CCG'CTC
Cuts at: 0 1439 2034 2157
Size: 1439 595 123
Fragments arranged by size:
1439 595 123
BsrDI GCAATG_nn'
Cuts at: 0 1727 2157
Size: 1727 430
BsrFI r'CCGG_y
Cuts at: 0 1301 1525 2157
Size: 1301 224 632
Fragments arranged by size:
1301 632 224
BssHII G'CGCG_C
Cuts at: 0 1428 2157
Size: 1428 729
BssSI C'ACGA_G
Cuts at: 0 34 709 2157
Size: 34 675 1448
Fragments arranged by size:
1448 675 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1800 1948 2072 2157
Size: 1800 148 124 85
Fragments arranged by size:
1800 148 124 85
BstEII G'GTnAC_C
Cuts at: 0 21 2157
Size: 21 2136
BstYI r'GATC_y
Cuts at: 0 128 1007 2157
Size: 128 879 1150
Fragments arranged by size:
1150 879 128
BtgI C'CryG_G
Cuts at: 0 564 850 1580 2157
Size: 564 286 730 577
Fragments arranged by size:
730 577 564 286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 941 2157
Size: 941 1216
BtsI GCAGTG_nn'
Cuts at: 0 18 719 1810 2076 2157
Size: 18 701 1091 266 81
Fragments arranged by size:
1091 701 266 81 18
DraIII CAC_nnn'GTG
Cuts at: 0 804 920 2157
Size: 804 116 1237
Fragments arranged by size:
1237 804 116
EaeI y'GGCC_r
Cuts at: 0 438 591 695 1577 2144 2157
Size: 438 153 104 882 567 13
Fragments arranged by size:
882 567 438 153 104 13
EagI C'GGCC_G
Cuts at: 0 1577 2157
Size: 1577 580
EarI CTCTTCn'nnn_
Cuts at: 0 749 2157
Size: 749 1408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 900 1189 1606 1663 1696 1700 1756
Size: 562 338 289 417 57 33 4 56
Cuts at: 1756 1888 2157
Size: 132 269
Fragments arranged by size:
562 417 338 289 269 132 57 56
33 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 544 697 766 2157
Size: 544 153 69 1391
Fragments arranged by size:
1391 544 153 69
EcoO109I rG'GnC_Cy
Cuts at: 0 230 531 759 875 1036 1204 1205 1239
Size: 230 301 228 116 161 168 1 34
Cuts at: 1239 1323 1626 1627 1748 1784 2157
Size: 84 303 1 121 36 373
Fragments arranged by size:
373 303 301 230 228 168 161 121
116 84 36 34 1 1
HaeII r_GCGC'y
Cuts at: 0 69 719 1311 1454 1569 2157
Size: 69 650 592 143 115 588
Fragments arranged by size:
650 592 588 143 115 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1131 1163 1600 1632 2157
Size: 1131 32 437 32 525
Fragments arranged by size:
1131 525 437 32 32
KasI G'GCGC_C
Cuts at: 0 65 715 1307 2157
Size: 65 650 592 850
Fragments arranged by size:
850 650 592 65
MluI A'CGCG_T
Cuts at: 0 776 2157
Size: 776 1381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1634 2157
Size: 36 1598 523
Fragments arranged by size:
1598 523 36
MscI TGG'CCA
Cuts at: 0 697 2146 2157
Size: 697 1449 11
Fragments arranged by size:
1449 697 11
MslI CAynn'nnrTG
Cuts at: 0 286 599 823 1579 2093 2157
Size: 286 313 224 756 514 64
Fragments arranged by size:
756 514 313 286 224 64
MspA1I CmG'CkG
Cuts at: 0 186 443 516 890 1085 1475 1662 1797
Size: 186 257 73 374 195 390 187 135
Cuts at: 1797 1977 2157
Size: 180 180
Fragments arranged by size:
390 374 257 195 187 186 180 180
135 73
NaeI GCC'GGC
Cuts at: 0 1527 2157
Size: 1527 630
NarI GG'CG_CC
Cuts at: 0 66 716 1308 2157
Size: 66 650 592 849
Fragments arranged by size:
849 650 592 66
NcoI C'CATG_G
Cuts at: 0 850 2157
Size: 850 1307
NgoMIV G'CCGG_C
Cuts at: 0 1525 2157
Size: 1525 632
PasI CC'CwG_GG
Cuts at: 0 1208 2157
Size: 1208 949
PflMI CCAn_nnn'nTGG
Cuts at: 0 36 693 1500 2091 2157
Size: 36 657 807 591 66
Fragments arranged by size:
807 657 591 66 36
PfoI T'CCnGG_A
Cuts at: 0 378 539 1865 2157
Size: 378 161 1326 292
Fragments arranged by size:
1326 378 292 161
PmlI CAC'GTG
Cuts at: 0 1353 2157
Size: 1353 804
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 2157
Size: 241 32 1884
Fragments arranged by size:
1884 241 32
PpuMI rG'GwC_Cy
Cuts at: 0 1784 2157
Size: 1784 373
PshAI GACnn'nnGTC
Cuts at: 0 290 2157
Size: 290 1867
PspOMI G'GGCC_C
Cuts at: 0 530 1035 1204 1239 1626 1748 2157
Size: 530 505 169 35 387 122 409
Fragments arranged by size:
530 505 409 387 169 122 35
PstI C_TGCA'G
Cuts at: 0 74 1230 1616 1805 2083 2157
Size: 74 1156 386 189 278 74
Fragments arranged by size:
1156 386 278 189 74 74
PvuII CAG'CTG
Cuts at: 0 186 890 1662 1797 1977 2157
Size: 186 704 772 135 180 180
Fragments arranged by size:
772 704 186 180 180 135
SexAI A'CCwGG_T
Cuts at: 0 1499 2157
Size: 1499 658
SfcI C'TryA_G
Cuts at: 0 70 1226 1612 1801 2079 2157
Size: 70 1156 386 189 278 78
Fragments arranged by size:
1156 386 278 189 78 70
SfoI GGC'GCC
Cuts at: 0 67 717 1309 2157
Size: 67 650 592 848
Fragments arranged by size:
848 650 592 67
SgrAI Cr'CCGG_yG
Cuts at: 0 1525 2157
Size: 1525 632
StuI AGG'CCT
Cuts at: 0 550 1717 2157
Size: 550 1167 440
Fragments arranged by size:
1167 550 440
StyI C'CwwG_G
Cuts at: 0 171 698 821 850 1077 1319 2157
Size: 171 527 123 29 227 242 838
Fragments arranged by size:
838 527 242 227 171 123 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1077 2157
Size: 1077 1080
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 766 798 2157
Size: 766 32 1359
Fragments arranged by size:
1359 766 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 2092 2157
Size: 33 2059 65
Fragments arranged by size:
2059 65 33
XmnI GAAnn'nnTTC
Cuts at: 0 523 2157
Size: 523 1634
ZraI GAC'GTC
Cuts at: 0 453 2157
Size: 453 1704
Enzymes that do cut:
AarI AatII AflIII AhdI AleI AloI AlwNI ApaI
ApaLI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI DraIII
EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuII SexAI SfcI SfoI SgrAI
StuI StyI TaqII TstI XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AlfI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BfrBI
BglI BmrI BmtI BpuEI BsaI BsaBI BsaWI BsiWI
BsmBI BspEI BspHI BsrGI BstBI BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmaI