Restriction map for
pF1KE0358
(Linear) (Six-base) MAPSORT of: KIEE0358.seq Check: 1130 from: 1 to: 2262
KIEE0358.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 578 1043 1327 2262
Size: 381 197 465 284 935
Fragments arranged by size:
935 465 381 284 197
AflII C'TTAA_G
Cuts at: 0 1732 2262
Size: 1732 530
AflIII A'CryG_T
Cuts at: 0 373 894 1297 1992 2262
Size: 373 521 403 695 270
Fragments arranged by size:
695 521 403 373 270
AleI CACnn'nnGTG
Cuts at: 0 1298 2262
Size: 1298 964
ApaLI G'TGCA_C
Cuts at: 0 1416 2262
Size: 1416 846
ApoI r'AATT_y
Cuts at: 0 674 905 1105 1603 2192 2262
Size: 674 231 200 498 589 70
Fragments arranged by size:
674 589 498 231 200 70
AvaI C'yCGr_G
Cuts at: 0 7 2262
Size: 7 2255
AvrII C'CTAG_G
Cuts at: 0 240 2262
Size: 240 2022
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2136 2169 2262
Size: 2136 33 93
Fragments arranged by size:
2136 93 33
BamHI G'GATC_C
Cuts at: 0 331 1737 2262
Size: 331 1406 525
Fragments arranged by size:
1406 525 331
BanI G'GyrC_C
Cuts at: 0 483 493 1965 2262
Size: 483 10 1472 297
Fragments arranged by size:
1472 483 297 10
BanII G_rGCy'C
Cuts at: 0 2110 2262
Size: 2110 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 569 1721 2262
Size: 569 1152 541
Fragments arranged by size:
1152 569 541
BciVI GTATCCnnnnn_n'
Cuts at: 0 1603 2137 2262
Size: 1603 534 125
Fragments arranged by size:
1603 534 125
BclI T'GATC_A
Cuts at: 0 855 2262
Size: 855 1407
BfrBI ATG'CAT
Cuts at: 0 1166 2262
Size: 1166 1096
BglII A'GATC_T
Cuts at: 0 1673 2262
Size: 1673 589
Bme1580I G_kGCm'C
Cuts at: 0 1420 2262
Size: 1420 842
BmrI ACTGGGnnnn_n'
Cuts at: 0 1018 2262
Size: 1018 1244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1716 2262
Size: 1716 546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 1924 2262
Size: 1706 218 338
Fragments arranged by size:
1706 338 218
Bpu10I CC'TnA_GC
Cuts at: 0 1262 2262
Size: 1262 1000
BsaAI yAC'GTr
Cuts at: 0 374 2262
Size: 374 1888
BsaBI GATnn'nnATC
Cuts at: 0 854 2246 2262
Size: 854 1392 16
Fragments arranged by size:
1392 854 16
BsaHI Gr'CG_yC
Cuts at: 0 1813 2262
Size: 1813 449
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1663 2262
Size: 1663 599
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1346 1665 2262
Size: 1082 264 319 597
Fragments arranged by size:
1082 597 319 264
BsiHKAI G_wGCw'C
Cuts at: 0 1420 1534 2262
Size: 1420 114 728
Fragments arranged by size:
1420 728 114
BsmI GAATG_Cn'
Cuts at: 0 1091 1342 1755 2262
Size: 1091 251 413 507
Fragments arranged by size:
1091 507 413 251
Bsp1286I G_dGCh'C
Cuts at: 0 1420 1534 2110 2262
Size: 1420 114 576 152
Fragments arranged by size:
1420 576 152 114
BspHI T'CATG_A
Cuts at: 0 1197 1566 2262
Size: 1197 369 696
Fragments arranged by size:
1197 696 369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2220 2262
Size: 2220 42
BsrDI GCAATG_nn'
Cuts at: 0 1917 2262
Size: 1917 345
BsrFI r'CCGG_y
Cuts at: 0 1383 2262
Size: 1383 879
BsrGI T'GTAC_A
Cuts at: 0 508 2262
Size: 508 1754
BssSI C'ACGA_G
Cuts at: 0 1517 2262
Size: 1517 745
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1213 2262
Size: 1213 1049
BstYI r'GATC_y
Cuts at: 0 226 331 830 960 1673 1737 2241 2262
Size: 226 105 499 130 713 64 504 21
Fragments arranged by size:
713 504 499 226 130 105 64 21
Bsu36I CC'TnA_GG
Cuts at: 0 41 478 1442 2262
Size: 41 437 964 820
Fragments arranged by size:
964 820 437 41
BtgI C'CryG_G
Cuts at: 0 1932 2262
Size: 1932 330
EaeI y'GGCC_r
Cuts at: 0 1422 1929 2262
Size: 1422 507 333
Fragments arranged by size:
1422 507 333
EarI CTCTTCn'nnn_
Cuts at: 0 1235 2262
Size: 1235 1027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 578 1043 1327 1706 1924 2262
Size: 381 197 465 284 379 218 338
Fragments arranged by size:
465 381 379 338 284 218 197
EcoRV GAT'ATC
Cuts at: 0 1572 2262
Size: 1572 690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 297 329 538 570 1516 1548 2262
Size: 297 32 209 32 946 32 714
Fragments arranged by size:
946 714 297 209 32 32 32
HincII GTy'rAC
Cuts at: 0 79 699 2262
Size: 79 620 1563
Fragments arranged by size:
1563 620 79
HindIII A'AGCT_T
Cuts at: 0 100 2262
Size: 100 2162
MfeI C'AATT_G
Cuts at: 0 1893 2262
Size: 1893 369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1208 1537 2262
Size: 1208 329 725
Fragments arranged by size:
1208 725 329
MscI TGG'CCA
Cuts at: 0 1424 1931 2262
Size: 1424 507 331
Fragments arranged by size:
1424 507 331
MslI CAynn'nnrTG
Cuts at: 0 137 143 347 727 1296 1298 2262
Size: 137 6 204 380 569 2 964
Fragments arranged by size:
964 569 380 204 137 6 2
MspA1I CmG'CkG
Cuts at: 0 358 2262
Size: 358 1904
NdeI CA'TA_TG
Cuts at: 0 1375 2262
Size: 1375 887
NsiI A_TGCA'T
Cuts at: 0 1168 2262
Size: 1168 1094
NspI r_CATG'y
Cuts at: 0 898 1166 1170 1301 1996 2262
Size: 898 268 4 131 695 266
Fragments arranged by size:
898 695 268 266 131 4
PasI CC'CwG_GG
Cuts at: 0 2124 2262
Size: 2124 138
PciI A'CATG_T
Cuts at: 0 894 1297 1992 2262
Size: 894 403 695 270
Fragments arranged by size:
894 695 403 270
PsiI TTA'TAA
Cuts at: 0 710 1154 2262
Size: 710 444 1108
Fragments arranged by size:
1108 710 444
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2128 2160 2262
Size: 2128 32 102
Fragments arranged by size:
2128 102 32
PvuII CAG'CTG
Cuts at: 0 358 2262
Size: 358 1904
ScaI AGT'ACT
Cuts at: 0 1141 2262
Size: 1141 1121
SfcI C'TryA_G
Cuts at: 0 1793 1970 2114 2262
Size: 1793 177 144 148
Fragments arranged by size:
1793 177 148 144
SmaI CCC'GGG
Cuts at: 0 9 2262
Size: 9 2253
SmlI C'TyrA_G
Cuts at: 0 1732 2262
Size: 1732 530
SphI G_CATG'C
Cuts at: 0 1170 2262
Size: 1170 1092
StyI C'CwwG_G
Cuts at: 0 63 240 327 1020 1707 2262
Size: 63 177 87 693 687 555
Fragments arranged by size:
693 687 555 177 87 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 2262
Size: 151 2111
TatI w'GTAC_w
Cuts at: 0 339 508 1139 1270 1302 2262
Size: 339 169 631 131 32 960
Fragments arranged by size:
960 631 339 169 131 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 282 314 1055 1087 2262
Size: 282 32 741 32 1175
Fragments arranged by size:
1175 741 282 32 32
XmaI C'CCGG_G
Cuts at: 0 7 2262
Size: 7 2255
Enzymes that do cut:
AcuI AflII AflIII AleI ApaLI ApoI AvaI AvrII
BaeI BamHI BanI BanII BbsI BciVI BclI BfrBI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaBI
BsaHI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI
EaeI EarI Eco57MI EcoRV Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NdeI NsiI NspI PasI
PciI PsiI PsrI PvuII ScaI SfcI SmaI SmlI
SphI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI AlwNI ApaI AscI AseI AsiSI BbeI
BbvCI BcgI BglI BlpI BmgBI BmtI BpuEI BsaI
BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmnI ZraI