Restriction map for
pF1KE0362
(Linear) (Six-base) MAPSORT of: KIEE0362.seq Check: 8144 from: 1 to: 1590
KIEE0362.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1418 1590
Size: 1418 172
AccI GT'mk_AC
Cuts at: 0 45 1590
Size: 45 1545
Acc65I G'GTAC_C
Cuts at: 0 1439 1590
Size: 1439 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1590
Size: 1271 319
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 1590
Size: 250 32 1308
Fragments arranged by size:
1308 250 32
ApaLI G'TGCA_C
Cuts at: 0 539 1590
Size: 539 1051
ApoI r'AATT_y
Cuts at: 0 698 856 929 1590
Size: 698 158 73 661
Fragments arranged by size:
698 661 158 73
AscI GG'CGCG_CC
Cuts at: 0 1400 1590
Size: 1400 190
AseI AT'TA_AT
Cuts at: 0 872 1590
Size: 872 718
BanI G'GyrC_C
Cuts at: 0 297 1439 1590
Size: 297 1142 151
Fragments arranged by size:
1142 297 151
BbsI GAAGACnn'nnnn_
Cuts at: 0 229 492 1590
Size: 229 263 1098
Fragments arranged by size:
1098 263 229
BciVI GTATCCnnnnn_n'
Cuts at: 0 791 1590
Size: 791 799
BclI T'GATC_A
Cuts at: 0 1176 1590
Size: 1176 414
Bme1580I G_kGCm'C
Cuts at: 0 95 543 550 1590
Size: 95 448 7 1040
Fragments arranged by size:
1040 448 95 7
BmgBI CAC'GTC
Cuts at: 0 1461 1590
Size: 1461 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 205 1590
Size: 205 1385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 902 1238 1305 1590
Size: 268 634 336 67 285
Fragments arranged by size:
634 336 285 268 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 927 1590
Size: 927 663
BsaHI Gr'CG_yC
Cuts at: 0 596 1590
Size: 596 994
BsaWI w'CCGG_w
Cuts at: 0 1532 1590
Size: 1532 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1205 1235 1590
Size: 1205 30 355
Fragments arranged by size:
1205 355 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 545 1590
Size: 545 1045
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1590
Size: 29 1561
BsiHKAI G_wGCw'C
Cuts at: 0 543 1590
Size: 543 1047
Bsp1286I G_dGCh'C
Cuts at: 0 95 543 550 1590
Size: 95 448 7 1040
Fragments arranged by size:
1040 448 95 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1418 1590
Size: 1418 172
BsrDI GCAATG_nn'
Cuts at: 0 301 1146 1590
Size: 301 845 444
Fragments arranged by size:
845 444 301
BssHII G'CGCG_C
Cuts at: 0 1400 1590
Size: 1400 190
BssSI C'ACGA_G
Cuts at: 0 5 1590
Size: 5 1585
BstEII G'GTnAC_C
Cuts at: 0 1075 1590
Size: 1075 515
Bsu36I CC'TnA_GG
Cuts at: 0 148 1590
Size: 148 1442
BtgI C'CryG_G
Cuts at: 0 95 1116 1151 1590
Size: 95 1021 35 439
Fragments arranged by size:
1021 439 95 35
BtsI GCAGTG_nn'
Cuts at: 0 437 1590
Size: 437 1153
DraI TTT'AAA
Cuts at: 0 1512 1590
Size: 1512 78
EaeI y'GGCC_r
Cuts at: 0 1361 1478 1590
Size: 1361 117 112
Fragments arranged by size:
1361 117 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 902 1238 1271 1305 1590
Size: 268 634 336 33 34 285
Fragments arranged by size:
634 336 285 268 34 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1330 1590
Size: 1330 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 905 937 1590
Size: 905 32 653
Fragments arranged by size:
905 653 32
KpnI G_GTAC'C
Cuts at: 0 1443 1590
Size: 1443 147
MfeI C'AATT_G
Cuts at: 0 953 1590
Size: 953 637
MslI CAynn'nnrTG
Cuts at: 0 1156 1590
Size: 1156 434
MspA1I CmG'CkG
Cuts at: 0 1353 1590
Size: 1353 237
NcoI C'CATG_G
Cuts at: 0 95 1116 1151 1590
Size: 95 1021 35 439
Fragments arranged by size:
1021 439 95 35
NdeI CA'TA_TG
Cuts at: 0 723 1135 1590
Size: 723 412 455
Fragments arranged by size:
723 455 412
PflMI CCAn_nnn'nTGG
Cuts at: 0 1455 1590
Size: 1455 135
SexAI A'CCwGG_T
Cuts at: 0 1435 1590
Size: 1435 155
SfcI C'TryA_G
Cuts at: 0 732 1590
Size: 732 858
SspI AAT'ATT
Cuts at: 0 478 515 1590
Size: 478 37 1075
Fragments arranged by size:
1075 478 37
StyI C'CwwG_G
Cuts at: 0 68 95 1116 1151 1454 1590
Size: 68 27 1021 35 303 136
Fragments arranged by size:
1021 303 136 68 35 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 404 1117 1362 1590
Size: 404 713 245 228
Fragments arranged by size:
713 404 245 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 1205 1237 1590
Size: 830 32 343 32 353
Fragments arranged by size:
830 353 343 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 185 1590
Size: 126 59 1405
Fragments arranged by size:
1405 126 59
Enzymes that do cut:
AarI AccI Acc65I AcuI AloI ApaLI ApoI AscI
AseI BanI BbsI BciVI BclI Bme1580I BmgBI BmrI
BpmI BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BssHII BssSI BstEII Bsu36I BtgI
BtsI DraI EaeI Eco57MI EcoNI Hin4I KpnI MfeI
MslI MspA1I NcoI NdeI PflMI SexAI SfcI SspI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AlwNI ApaI AsiSI AvaI AvrII BaeI BamHI
BanII BbeI BbvCI BcgI BfrBI BglI BglII BlpI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BstAPI BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI
DraIII DrdI EagI EarI EciI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MluI MmeI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI