Restriction map for
pF1KE0363
(Linear) (Six-base) MAPSORT of: KIEE0363.seq Check: 934 from: 1 to: 2235
KIEE0363.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 0 2014 2235
Size: 2014 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 616 746 914 1376 1910 2235
Size: 452 164 130 168 462 534 325
Fragments arranged by size:
534 462 452 325 168 164 130
AflIII A'CryG_T
Cuts at: 0 2100 2235
Size: 2100 135
AgeI A'CCGG_T
Cuts at: 0 280 2235
Size: 280 1955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 320 2235
Size: 320 1915
AlwNI CAG_nnn'CTG
Cuts at: 0 208 2235
Size: 208 2027
ApoI r'AATT_y
Cuts at: 0 526 1087 1351 1921 2235
Size: 526 561 264 570 314
Fragments arranged by size:
570 561 526 314 264
BanII G_rGCy'C
Cuts at: 0 29 2235
Size: 29 2206
BbsI GAAGACnn'nnnn_
Cuts at: 0 283 898 2235
Size: 283 615 1337
Fragments arranged by size:
1337 615 283
BclI T'GATC_A
Cuts at: 0 2073 2235
Size: 2073 162
BfrBI ATG'CAT
Cuts at: 0 1328 2235
Size: 1328 907
BlpI GC'TnA_GC
Cuts at: 0 2190 2235
Size: 2190 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 1011 1953 2235
Size: 1011 942 282
Fragments arranged by size:
1011 942 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1836 2235
Size: 1836 399
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 2235
Size: 211 2024
BsaI GGTCTCn'nnnn_
Cuts at: 0 584 832 1379 2051 2235
Size: 584 248 547 672 184
Fragments arranged by size:
672 584 547 248 184
BsaBI GATnn'nnATC
Cuts at: 0 948 2235
Size: 948 1287
BsaWI w'CCGG_w
Cuts at: 0 280 2235
Size: 280 1955
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 461 491 2105 2135 2235
Size: 461 30 1614 30 100
Fragments arranged by size:
1614 461 100 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 5 485 1520 1733 2235
Size: 5 480 1035 213 502
Fragments arranged by size:
1035 502 480 213 5
BseYI C'CCAG_C
Cuts at: 0 292 2195 2235
Size: 292 1903 40
Fragments arranged by size:
1903 292 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 550 1241 1691 2235
Size: 194 356 691 450 544
Fragments arranged by size:
691 544 450 356 194
BsmI GAATG_Cn'
Cuts at: 0 878 2235
Size: 878 1357
Bsp1286I G_dGCh'C
Cuts at: 0 29 2235
Size: 29 2206
BspHI T'CATG_A
Cuts at: 0 138 2235
Size: 138 2097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1053 2235
Size: 1053 1182
BsrDI GCAATG_nn'
Cuts at: 0 314 1920 2123 2235
Size: 314 1606 203 112
Fragments arranged by size:
1606 314 203 112
BsrFI r'CCGG_y
Cuts at: 0 280 2235
Size: 280 1955
BstAPI GCAn_nnn'nTGC
Cuts at: 0 208 1325 2122 2235
Size: 208 1117 797 113
Fragments arranged by size:
1117 797 208 113
BstYI r'GATC_y
Cuts at: 0 204 2235
Size: 204 2031
BstZ17I GTA'TAC
Cuts at: 0 2015 2235
Size: 2015 220
Bsu36I CC'TnA_GG
Cuts at: 0 17 1772 2235
Size: 17 1755 463
Fragments arranged by size:
1755 463 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1338 2235
Size: 1338 897
BtsI GCAGTG_nn'
Cuts at: 0 1364 1751 2235
Size: 1364 387 484
Fragments arranged by size:
1364 484 387
DraI TTT'AAA
Cuts at: 0 335 2235
Size: 335 1900
EarI CTCTTCn'nnn_
Cuts at: 0 110 1156 1391 1886 2235
Size: 110 1046 235 495 349
Fragments arranged by size:
1046 495 349 235 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 616 746 914 1376 1836 1910 2235
Size: 452 164 130 168 462 460 74 325
Fragments arranged by size:
462 460 452 325 168 164 130 74
EcoRV GAT'ATC
Cuts at: 0 1812 2235
Size: 1812 423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 883 1473 2235
Size: 883 590 762
Fragments arranged by size:
883 762 590
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 2235
Size: 754 32 1449
Fragments arranged by size:
1449 754 32
HincII GTy'rAC
Cuts at: 0 1593 2235
Size: 1593 642
HindIII A'AGCT_T
Cuts at: 0 2187 2235
Size: 2187 48
MfeI C'AATT_G
Cuts at: 0 1232 2235
Size: 1232 1003
MslI CAynn'nnrTG
Cuts at: 0 847 2235
Size: 847 1388
MspA1I CmG'CkG
Cuts at: 0 56 74 2010 2235
Size: 56 18 1936 225
Fragments arranged by size:
1936 225 56 18
NdeI CA'TA_TG
Cuts at: 0 774 1985 1994 2235
Size: 774 1211 9 241
Fragments arranged by size:
1211 774 241 9
NsiI A_TGCA'T
Cuts at: 0 1330 2235
Size: 1330 905
NspI r_CATG'y
Cuts at: 0 1336 2104 2235
Size: 1336 768 131
Fragments arranged by size:
1336 768 131
PasI CC'CwG_GG
Cuts at: 0 29 2235
Size: 29 2206
PciI A'CATG_T
Cuts at: 0 2100 2235
Size: 2100 135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 2235
Size: 335 32 1868
Fragments arranged by size:
1868 335 32
SapI GCTCTTCn'nnn_
Cuts at: 0 1156 2235
Size: 1156 1079
SfcI C'TryA_G
Cuts at: 0 2174 2235
Size: 2174 61
SmlI C'TyrA_G
Cuts at: 0 226 2235
Size: 226 2009
SspI AAT'ATT
Cuts at: 0 996 1409 1644 1953 2030 2235
Size: 996 413 235 309 77 205
Fragments arranged by size:
996 413 309 235 205 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 50 2235
Size: 50 2185
TatI w'GTAC_w
Cuts at: 0 443 2235
Size: 443 1792
Enzymes that do cut:
AccI AcuI AflIII AgeI AlfI AlwNI ApoI BanII
BbsI BclI BfrBI BlpI BmrI BpmI BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BstAPI BstYI BstZ17I Bsu36I
BtgZI BtsI DraI EarI Eco57MI EcoRV FalI Hin4I
HincII HindIII MfeI MslI MspA1I NdeI NsiI NspI
PasI PciI PpiI SapI SfcI SmlI SspI TaqII
TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI
BglII Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaHI
BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstXI BtgI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI