Restriction map for
pF1KE0366
(Linear) (Six-base) MAPSORT of: KIEE0366.seq Check: 2926 from: 1 to: 1902
KIEE0366.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 139 1902
Size: 139 1763
AccI GT'mk_AC
Cuts at: 0 962 1517 1902
Size: 962 555 385
Fragments arranged by size:
962 555 385
Acc65I G'GTAC_C
Cuts at: 0 527 1902
Size: 527 1375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 751 993 1073 1902
Size: 352 399 242 80 829
Fragments arranged by size:
829 399 352 242 80
AfeI AGC'GCT
Cuts at: 0 994 1902
Size: 994 908
AflIII A'CryG_T
Cuts at: 0 161 626 1520 1902
Size: 161 465 894 382
Fragments arranged by size:
894 465 382 161
AleI CACnn'nnGTG
Cuts at: 0 940 1902
Size: 940 962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 1902
Size: 268 1634
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1540 1572 1902
Size: 1540 32 330
Fragments arranged by size:
1540 330 32
AlwNI CAG_nnn'CTG
Cuts at: 0 285 1671 1685 1854 1902
Size: 285 1386 14 169 48
Fragments arranged by size:
1386 285 169 48 14
ApaLI G'TGCA_C
Cuts at: 0 630 883 1902
Size: 630 253 1019
Fragments arranged by size:
1019 630 253
ApoI r'AATT_y
Cuts at: 0 1705 1902
Size: 1705 197
AvaI C'yCGr_G
Cuts at: 0 567 1902
Size: 567 1335
AvrII C'CTAG_G
Cuts at: 0 339 1902
Size: 339 1563
BamHI G'GATC_C
Cuts at: 0 41 1902
Size: 41 1861
BanI G'GyrC_C
Cuts at: 0 527 736 1902
Size: 527 209 1166
Fragments arranged by size:
1166 527 209
BanII G_rGCy'C
Cuts at: 0 316 508 574 1902
Size: 316 192 66 1328
Fragments arranged by size:
1328 316 192 66
BbeI G_GCGC'C
Cuts at: 0 740 1902
Size: 740 1162
BbsI GAAGACnn'nnnn_
Cuts at: 0 747 816 1197 1902
Size: 747 69 381 705
Fragments arranged by size:
747 705 381 69
BclI T'GATC_A
Cuts at: 0 1628 1902
Size: 1628 274
BglII A'GATC_T
Cuts at: 0 1724 1902
Size: 1724 178
BlpI GC'TnA_GC
Cuts at: 0 56 1902
Size: 56 1846
Bme1580I G_kGCm'C
Cuts at: 0 634 887 1186 1325 1778 1902
Size: 634 253 299 139 453 124
Fragments arranged by size:
634 453 299 253 139 124
BmgBI CAC'GTC
Cuts at: 0 362 554 1902
Size: 362 192 1348
Fragments arranged by size:
1348 362 192
BmrI ACTGGGnnnn_n'
Cuts at: 0 1749 1902
Size: 1749 153
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 105 556 921 1524 1902
Size: 105 451 365 603 378
Fragments arranged by size:
603 451 378 365 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 259 283 468 852 1790 1902
Size: 82 177 24 185 384 938 112
Fragments arranged by size:
938 384 185 177 112 82 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 58 643 1902
Size: 58 585 1259
Fragments arranged by size:
1259 585 58
BsaAI yAC'GTr
Cuts at: 0 1521 1598 1902
Size: 1521 77 304
Fragments arranged by size:
1521 304 77
BsaHI Gr'CG_yC
Cuts at: 0 35 737 1313 1902
Size: 35 702 576 589
Fragments arranged by size:
702 589 576 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 661 691 1429 1459 1902
Size: 556 30 75 30 738 30 443
Fragments arranged by size:
738 556 443 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 212 215 1902
Size: 94 118 3 1687
Fragments arranged by size:
1687 118 94 3
BseYI C'CCAG_C
Cuts at: 0 788 1902
Size: 788 1114
BsiEI CG_ry'CG
Cuts at: 0 97 1902
Size: 97 1805
BsiHKAI G_wGCw'C
Cuts at: 0 316 508 634 887 1256 1373 1864 1902
Size: 316 192 126 253 369 117 491 38
Fragments arranged by size:
491 369 316 253 192 126 117 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 529 1902
Size: 529 1373
Bsp1286I G_dGCh'C
Cuts at: 0 316 508 574 634 887 1186 1256 1325
Size: 316 192 66 60 253 299 70 69
Cuts at: 1325 1373 1778 1864 1902
Size: 48 405 86 38
Fragments arranged by size:
405 316 299 253 192 86 70 69
66 60 48 38
BspHI T'CATG_A
Cuts at: 0 209 1559 1902
Size: 209 1350 343
Fragments arranged by size:
1350 343 209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 139 1055 1153 1902
Size: 139 916 98 749
Fragments arranged by size:
916 749 139 98
BsrBI CCG'CTC
Cuts at: 0 462 1902
Size: 462 1440
BsrFI r'CCGG_y
Cuts at: 0 1465 1902
Size: 1465 437
BsrGI T'GTAC_A
Cuts at: 0 623 668 1902
Size: 623 45 1234
Fragments arranged by size:
1234 623 45
BssSI C'ACGA_G
Cuts at: 0 10 1902
Size: 10 1892
BstAPI GCAn_nnn'nTGC
Cuts at: 0 123 285 1902
Size: 123 162 1617
Fragments arranged by size:
1617 162 123
BstYI r'GATC_y
Cuts at: 0 41 1724 1902
Size: 41 1683 178
Fragments arranged by size:
1683 178 41
BtgI C'CryG_G
Cuts at: 0 192 639 1115 1175 1902
Size: 192 447 476 60 727
Fragments arranged by size:
727 476 447 192 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1473 1902
Size: 1473 429
BtsI GCAGTG_nn'
Cuts at: 0 1824 1902
Size: 1824 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1579 1614 1902
Size: 1579 35 288
Fragments arranged by size:
1579 288 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1339 1902
Size: 1339 563
EaeI y'GGCC_r
Cuts at: 0 94 950 1431 1566 1902
Size: 94 856 481 135 336
Fragments arranged by size:
856 481 336 135 94
EagI C'GGCC_G
Cuts at: 0 94 1902
Size: 94 1808
EarI CTCTTCn'nnn_
Cuts at: 0 766 1261 1276 1645 1902
Size: 766 495 15 369 257
Fragments arranged by size:
766 495 369 257 15
EcoICRI GAG'CTC
Cuts at: 0 314 506 1902
Size: 314 192 1396
Fragments arranged by size:
1396 314 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 259 283 352 468 751 852 993
Size: 82 177 24 69 116 283 101 141
Cuts at: 993 1073 1790 1902
Size: 80 717 112
Fragments arranged by size:
717 283 177 141 116 112 101 82
80 69 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1168 1902
Size: 1168 734
EcoO109I rG'GnC_Cy
Cuts at: 0 336 852 1332 1689 1890 1902
Size: 336 516 480 357 201 12
Fragments arranged by size:
516 480 357 336 201 12
FspI TGC'GCA
Cuts at: 0 609 1902
Size: 609 1293
HaeII r_GCGC'y
Cuts at: 0 171 740 996 1902
Size: 171 569 256 906
Fragments arranged by size:
906 569 256 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 1902
Size: 556 32 1314
Fragments arranged by size:
1314 556 32
KasI G'GCGC_C
Cuts at: 0 736 1902
Size: 736 1166
KpnI G_GTAC'C
Cuts at: 0 531 1902
Size: 531 1371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1133 1902
Size: 1133 769
MscI TGG'CCA
Cuts at: 0 952 1902
Size: 952 950
MslI CAynn'nnrTG
Cuts at: 0 940 1225 1902
Size: 940 285 677
Fragments arranged by size:
940 677 285
MspA1I CmG'CkG
Cuts at: 0 282 641 1095 1117 1131 1902
Size: 282 359 454 22 14 771
Fragments arranged by size:
771 454 359 282 22 14
NaeI GCC'GGC
Cuts at: 0 1467 1902
Size: 1467 435
NarI GG'CG_CC
Cuts at: 0 737 1902
Size: 737 1165
NgoMIV G'CCGG_C
Cuts at: 0 1465 1902
Size: 1465 437
NspI r_CATG'y
Cuts at: 0 126 630 1089 1902
Size: 126 504 459 813
Fragments arranged by size:
813 504 459 126
PciI A'CATG_T
Cuts at: 0 626 1902
Size: 626 1276
PflMI CCAn_nnn'nTGG
Cuts at: 0 581 1902
Size: 581 1321
PfoI T'CCnGG_A
Cuts at: 0 236 400 431 1902
Size: 236 164 31 1471
Fragments arranged by size:
1471 236 164 31
PmlI CAC'GTG
Cuts at: 0 1598 1902
Size: 1598 304
PpuMI rG'GwC_Cy
Cuts at: 0 1332 1689 1890 1902
Size: 1332 357 201 12
Fragments arranged by size:
1332 357 201 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 250 282 1902
Size: 250 32 1620
Fragments arranged by size:
1620 250 32
PstI C_TGCA'G
Cuts at: 0 1331 1902
Size: 1331 571
PvuII CAG'CTG
Cuts at: 0 1131 1902
Size: 1131 771
SacI G_AGCT'C
Cuts at: 0 316 508 1902
Size: 316 192 1394
Fragments arranged by size:
1394 316 192
SacII CC_GC'GG
Cuts at: 0 642 1118 1902
Size: 642 476 784
Fragments arranged by size:
784 642 476
SanDI GG'GwC_CC
Cuts at: 0 1689 1902
Size: 1689 213
SapI GCTCTTCn'nnn_
Cuts at: 0 1261 1276 1902
Size: 1261 15 626
Fragments arranged by size:
1261 626 15
SbfI CC_TGCA'GG
Cuts at: 0 1331 1902
Size: 1331 571
ScaI AGT'ACT
Cuts at: 0 1438 1902
Size: 1438 464
SfcI C'TryA_G
Cuts at: 0 1327 1677 1902
Size: 1327 350 225
Fragments arranged by size:
1327 350 225
SfoI GGC'GCC
Cuts at: 0 738 1902
Size: 738 1164
StuI AGG'CCT
Cuts at: 0 344 722 1190 1379 1902
Size: 344 378 468 189 523
Fragments arranged by size:
523 468 378 344 189
StyI C'CwwG_G
Cuts at: 0 339 1361 1902
Size: 339 1022 541
Fragments arranged by size:
1022 541 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 1731 1902
Size: 292 1439 171
Fragments arranged by size:
1439 292 171
TatI w'GTAC_w
Cuts at: 0 119 623 668 1436 1902
Size: 119 504 45 768 466
Fragments arranged by size:
768 504 466 119 45
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstYI BtgI BtgZI BtsI
CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmlI PpuMI PsrI PstI PvuII SacI SacII
SanDI SapI SbfI ScaI SfcI SfoI StuI StyI
TaqII TatI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI ApaI AscI AseI
AsiSI BaeI BbvCI BcgI BciVI BfrBI BglI BmtI
Bpu10I BpuEI BsaBI BsaWI BsgI BsiWI BsmI BspEI
BsrDI BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI
DraI DraIII EciI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SalI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI