Restriction map for
pF1KE0367
(Linear) (Six-base) MAPSORT of: KIEE0367.seq Check: 827 from: 1 to: 2262
KIEE0367.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 487 1048 1566 2262
Size: 15 472 561 518 696
Fragments arranged by size:
696 561 518 472 15
AfeI AGC'GCT
Cuts at: 0 679 2262
Size: 679 1583
AflII C'TTAA_G
Cuts at: 0 430 2262
Size: 430 1832
AhdI GACnn_n'nnGTC
Cuts at: 0 387 2262
Size: 387 1875
AleI CACnn'nnGTG
Cuts at: 0 312 2262
Size: 312 1950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 302 773 2262
Size: 302 471 1489
Fragments arranged by size:
1489 471 302
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 994 1026 2262
Size: 994 32 1236
Fragments arranged by size:
1236 994 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2115 2262
Size: 2115 147
ApoI r'AATT_y
Cuts at: 0 668 696 819 915 1775 2262
Size: 668 28 123 96 860 487
Fragments arranged by size:
860 668 487 123 96 28
AvaI C'yCGr_G
Cuts at: 0 179 2262
Size: 179 2083
AvrII C'CTAG_G
Cuts at: 0 1782 2262
Size: 1782 480
BbsI GAAGACnn'nnnn_
Cuts at: 0 445 901 1566 2262
Size: 445 456 665 696
Fragments arranged by size:
696 665 456 445
BciVI GTATCCnnnnn_n'
Cuts at: 0 341 2262
Size: 341 1921
BclI T'GATC_A
Cuts at: 0 80 119 2262
Size: 80 39 2143
Fragments arranged by size:
2143 80 39
BglII A'GATC_T
Cuts at: 0 1318 2262
Size: 1318 944
Bme1580I G_kGCm'C
Cuts at: 0 762 2262
Size: 762 1500
BmrI ACTGGGnnnn_n'
Cuts at: 0 789 2262
Size: 789 1473
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2211 2262
Size: 2211 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 2262
Size: 92 2170
BsaBI GATnn'nnATC
Cuts at: 0 653 2262
Size: 653 1609
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2184 2262
Size: 2184 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 2101 2262
Size: 1498 603 161
Fragments arranged by size:
1498 603 161
Bsp1286I G_dGCh'C
Cuts at: 0 762 2262
Size: 762 1500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1294 2262
Size: 1294 968
BstYI r'GATC_y
Cuts at: 0 930 1318 2262
Size: 930 388 944
Fragments arranged by size:
944 930 388
BtsI GCAGTG_nn'
Cuts at: 0 1486 2262
Size: 1486 776
DraI TTT'AAA
Cuts at: 0 21 975 2262
Size: 21 954 1287
Fragments arranged by size:
1287 954 21
DrdI GACnn_nn'nnGTC
Cuts at: 0 423 2262
Size: 423 1839
EaeI y'GGCC_r
Cuts at: 0 1502 2262
Size: 1502 760
EarI CTCTTCn'nnn_
Cuts at: 0 34 224 746 1887 1990 2202 2262
Size: 34 190 522 1141 103 212 60
Fragments arranged by size:
1141 522 212 190 103 60 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 238 2262
Size: 238 2024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 487 1048 1566 2262
Size: 15 472 561 518 696
Fragments arranged by size:
696 561 518 472 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1230 2262
Size: 1230 1032
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1473 2262
Size: 1473 789
HaeII r_GCGC'y
Cuts at: 0 681 2262
Size: 681 1581
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 2262
Size: 373 32 1857
Fragments arranged by size:
1857 373 32
HincII GTy'rAC
Cuts at: 0 2046 2262
Size: 2046 216
HindIII A'AGCT_T
Cuts at: 0 985 2262
Size: 985 1277
HpaI GTT'AAC
Cuts at: 0 2046 2262
Size: 2046 216
MscI TGG'CCA
Cuts at: 0 1504 2262
Size: 1504 758
MslI CAynn'nnrTG
Cuts at: 0 194 284 312 1111 1312 1636 1813 2262
Size: 194 90 28 799 201 324 177 449
Fragments arranged by size:
799 449 324 201 194 177 90 28
MspA1I CmG'CkG
Cuts at: 0 2112 2262
Size: 2112 150
PflMI CCAn_nnn'nTGG
Cuts at: 0 1949 2180 2262
Size: 1949 231 82
Fragments arranged by size:
1949 231 82
PfoI T'CCnGG_A
Cuts at: 0 529 2017 2262
Size: 529 1488 245
Fragments arranged by size:
1488 529 245
PpuMI rG'GwC_Cy
Cuts at: 0 1230 2262
Size: 1230 1032
PshAI GACnn'nnGTC
Cuts at: 0 1615 2262
Size: 1615 647
PvuII CAG'CTG
Cuts at: 0 2112 2262
Size: 2112 150
SapI GCTCTTCn'nnn_
Cuts at: 0 746 2262
Size: 746 1516
SmlI C'TyrA_G
Cuts at: 0 107 430 2262
Size: 107 323 1832
Fragments arranged by size:
1832 323 107
SpeI A'CTAG_T
Cuts at: 0 247 2262
Size: 247 2015
SspI AAT'ATT
Cuts at: 0 566 611 1259 2262
Size: 566 45 648 1003
Fragments arranged by size:
1003 648 566 45
StyI C'CwwG_G
Cuts at: 0 1782 2262
Size: 1782 480
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 1381 2262
Size: 771 610 881
Fragments arranged by size:
881 771 610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1339 1371 1883 1915 2262
Size: 1339 32 512 32 347
Fragments arranged by size:
1339 512 347 32 32
XbaI T'CTAG_A
Cuts at: 0 645 1071 2262
Size: 645 426 1191
Fragments arranged by size:
1191 645 426
XmnI GAAnn'nnTTC
Cuts at: 0 740 2262
Size: 740 1522
Enzymes that do cut:
AcuI AfeI AflII AhdI AleI AlfI AloI AlwNI
ApoI AvaI AvrII BbsI BciVI BclI BglII Bme1580I
BmrI BplI BpuEI BsaBI BseRI BsgI Bsp1286I BspMI
BstYI BtsI DraI DrdI EaeI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII HpaI MscI
MslI MspA1I PflMI PfoI PpuMI PshAI PvuII SapI
SmlI SpeI SspI StyI TaqII TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI ApaI
ApaLI AscI AseI AsiSI BaeI BamHI BanI BanII
BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmtI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI SwaI TatI Tth111I
XcmI XhoI XmaI ZraI